FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_8561_flu.3520000003eb53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_8561_flu.3520000003eb53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389415
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC169384.34960132506452No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT114612.943132647689483No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA95462.45136936173491No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG93422.398983090019645No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG82212.1111153910352707No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA68031.7469794435242607No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT57061.465274835329918No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT53241.367178973588588No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT49971.283206861574413No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA44371.1394014098070182No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA43681.1216825237856785No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT33040.8484521654276286No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA32280.8289357112591964No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG20460.5254034898501599No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG19730.5066574220304816No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT14510.372610197347303Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT13390.34384910699382404No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT13140.3374292207542083No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA9680.24857799519792512No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8220.21108585955856865No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7490.19233979173889038No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT6750.17333692846962753No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5900.1515093152549337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA71150.0292.92691
GGGTTAT72050.0288.653722
GTTATTA72800.0284.46434
GGTTATT72950.0284.081573
GGGGGGA48100.0283.040531
TTATTAG73850.0280.61955
GGGGAGC49400.0277.085023
GGGAGCG38400.0276.946624
TTAGTAG75000.0275.923348
GGAGCGA38500.0275.461065
ATTAGTA75600.0273.733467
TATTAGT75900.0273.234536
CGAAAGC39200.0268.284459
TAGTAGA78050.0263.62919
GAGCGAA40250.0263.1186
AGCGAAA41100.0255.8827
GCGAAAG42050.0249.399528
GGGGGAG55900.0246.712882
GGGAGCA14650.0211.433464
AAACGTT2550.0161.96077295
Produced by FastQC (version 0.11.2)