FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_8033_flu.3520000003eb02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_8033_flu.3520000003eb02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505243
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT89441.770237291758619No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG57511.1382641619972964No Hit
GGGGGGAGCAGAAGCAGAGGATTGAAGCAGTACCGTAAGGAGTTGTGGAC55901.1063983073491368No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCTACCT37680.7457797535047492No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA37680.7457797535047492No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT36580.7240080515712242No Hit
CCGGGTTATTAGTAGAAACAACAGCTTCAACAACAGCTGCTACAGCAACA35520.7030280478898272No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT34010.6731414388719883No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT31800.6294001104419061No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT30750.6086180313235413No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA30640.6064408611301889No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT29320.5803148188099588No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT28600.5660642502716515No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG28440.5628974572631387No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA28070.555574248430953No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC28050.5551783993048889No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG26090.5163851849506079No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA22760.4504763054609366No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC21810.43167347197289224No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT21160.4188083753758093No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT20210.40000554188776494No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC19670.38931761548403443No Hit
CCGGGTTATTAGTAGAAACACGAGCATAGTAGTTTGTATTGTAACGGGTA18860.37328572587843867No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA18360.3633894977268364No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA18340.3629936486007723No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA17260.3416177957933113No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT17000.33647175715447813Illumina Single End PCR Primer 1 (97% over 34bp)
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCCACCT15780.31232496046456854No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC14910.2951055234807805No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG13980.27669853911880027No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT13690.27095872679087096No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA12640.25017664767250614No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA12270.2428534388403204No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG12080.23909287214271152No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA11430.22622777554562853No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA11380.2252381527304683No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10780.21336267894854552No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT8900.1761528610985209No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7900.1563604047953163No Hit
CCGGGTTATTAGTAGAAACACGTGCATAGTAGTTTGTATTGTAACGGGTA7460.1476517240219063No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA7180.14210983625700901No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA6770.13399492917269512No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG6740.133401155483599No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGATTGGTTTGGTGATGATAATG6390.12647379577747736No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA6200.1227132290798685No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT5490.10866058510459323No Hit
GGGGGGAGCAGAAGCAGAGGATCTTCTCAAAAAACTGAGGCAAATAGGCC5180.10252492365059981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT76650.0290.95891
CGGGTTA78700.0283.754762
TTATTAG85600.0261.226626
GGGGGGA63250.0260.952581
GTTATTA88550.0251.857715
GGGTTAT89500.0249.678793
TATTAGT90350.0247.329827
ATTAGTA90050.0245.369238
GGGGAGC68150.0244.57083
GGGAGCA68250.0243.780234
GGAGCAG68400.0243.029975
GAGCAGA70100.0237.136236
TTAGTAG95850.0230.675529
CAGAAGC72150.0230.398489
GGTTATT98500.0227.314734
GGGGGAG74500.0224.318792
GCAGAAG79100.0210.341348
AGCAGAA93500.0177.473277
CCGCCAA4400.0107.27272295
CCGGTTA856.510163E-9104.1176451
Produced by FastQC (version 0.11.2)