FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_7965_flu.3520000003e9e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_7965_flu.3520000003e9e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277472
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC111834.030316572482989No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT81492.9368729096989967No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG67612.4366422557951797No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA51951.8722609848921694No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA44981.621064467766117No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA36451.3136460615845924No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT34831.2552617921808327No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT33451.2055270441702226No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG27160.9788375043247607No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT26270.9467621958251644No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG20990.7564727251758736No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG19530.703854803367547No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18060.6508764848345059No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA14600.5261792180832661No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA13240.4771652635220851No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG12350.44508995502248877No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT10350.3730106100795756No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9030.32543824241725294No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT8380.3020124553108061Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG7910.28507380924922154No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT7720.2782262714796448No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7000.25227770730019605No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5870.21155287740745013No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA4130.1488438473071157No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4010.1445190866105409No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA2950.10631703379079692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA42050.0293.59691
GGGTTAT42150.0292.900362
GTTATTA42750.0287.754364
GGGGGGA31000.0284.056461
GGTTATT43800.0282.20323
TTATTAG43650.0280.807565
TTAGTAG44950.0272.35828
GGGGAGC32200.0270.722053
GGGAGCG25200.0264.56354
TATTAGT46400.0264.482766
TAGTAGA46200.0263.712139
GGAGCGA25200.0262.807565
ATTAGTA47300.0259.762187
CGAAAGC25850.0256.769849
GCGAAAG26950.0246.836758
GGGGGAG36850.0238.9622
GAGCGAA27900.0236.845896
AGCGAAA28150.0236.314397
GGGAGCA11000.0186.386374
CAAAAGC14800.0131.554059
Produced by FastQC (version 0.11.2)