FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_7765_flu.3520000003ea47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_7765_flu.3520000003ea47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357397
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA149304.1774273427029325No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC143084.0033911868314505No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC92362.584241054065927No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG88202.46784388229336No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG70391.9695184906420593No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA68151.906843090456831No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG57201.6004611118727914No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG38481.0766738388962414No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC34620.9686706939341965No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA33080.925581356306852No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG25430.7115336726385504No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG24910.6969840261669796No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA23520.658091701944896No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA21910.6130437580617633No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA18150.5078386220365587No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT14940.4180225351639773No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA13260.37101598502505617No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC12810.3584249448092737No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT10040.28092009725879064Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA8930.24986219805986062No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA6120.17123814693464132No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT5770.16144511565569938No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4530.12674980483887666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA62750.0292.414341
GGGTTAT63000.0291.01982
TTATTAG64700.0282.233375
ATTAGTA64600.0282.213627
GGTTATT64900.0281.818183
TATTAGT65200.0279.842776
GGGGGGA44700.0279.8211
GTTATTA66700.0273.991734
TTAGTAG68650.0266.209058
TAGTAGA69150.0263.857549
GGAGCGA34800.0262.36355
GGGGGAG47850.0260.475462
CGAAAGC35000.0259.69
GAGCGAA35400.0256.666666
GCGAAAG35850.0253.44498
GGGGAGC53750.0230.786033
AGCGAAA40550.0225.16037
GGGAGCG40950.0224.761924
GAGCAAA19400.0158.904656
GCAAAAG19250.0152.480518
Produced by FastQC (version 0.11.2)