FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_7637_flu.3520000003ea54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_7637_flu.3520000003ea54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389112
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC147163.781944530109583No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG111512.8657558749151915No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT105332.7069327083204837No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA64351.6537654968235365No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA56811.4599909537613849No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA50131.2883180164065873No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG48671.2507966857871256No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT47821.2289520754949732No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT31230.8025966816751989No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT30140.7745841814182035No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA29650.7619914060733156No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG28350.7285820020970826No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG26890.6910606714776208No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA22480.5777256933736302No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT19150.4921462201114332No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18660.4795534447665454No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT12850.3302391085343037No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT9480.24363165361129957Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9400.2415756902896852No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9360.24054770862887803No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7860.2019983963486091No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA7020.18041078147165857No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC6500.16704701988116533No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA6470.16627603363555996No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG6410.1647340611443492No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5210.13389461132013405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA55500.0292.873871
GGGTTAT56150.0290.00892
GTTATTA57350.0283.426334
GGTTATT57800.0281.47493
TTATTAG58150.0279.780735
TTAGTAG58550.0276.35788
GGGGGGA43800.0276.141571
TAGTAGA59100.0273.28689
TATTAGT59600.0272.479036
GGGGAGC44350.0271.38673
ATTAGTA61000.0265.983587
GGGAGCG34800.0261.939644
GGAGCGA34700.0261.41935
CGAAAGC36650.0246.705329
AGCGAAA37800.0238.41937
GAGCGAA38050.0238.015786
GCGAAAG38600.0234.242238
GGGGGAG52450.0230.600572
GGGAGCA14600.0188.921234
AAACGTT1250.0188.8295
Produced by FastQC (version 0.11.2)