FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_7331_flu.3520000003eb46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_7331_flu.3520000003eb46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577051
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA266624.6203888391147405No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC218823.7920391785128174No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG121322.1024138247745867No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC118992.062036111192945No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA101931.7663949980157734No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG101211.7539177646343218No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG89601.5527223763584155No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA59711.0347438961201003No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG56250.9747838579259026No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC54130.9380453374138507No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG51410.890909122417256No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA44370.7689095071319519No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC35880.621782130175669No Hit
GGGGGGAGCGAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT35550.6160633982091704No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT31090.538773869207401No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG29510.511393273731438No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA25930.4493536966403316No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG25010.43341056509736575No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC24790.4295980771197No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA23810.4126151761282798No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT15260.26444802972354264Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC14200.24607876946751675No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT13770.23862708842026092No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA12470.21609875037041787No Hit
GGGGGGAGCAAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT10530.18247953820372895No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA9860.170868779362656No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTCAACTGTCATACTCCT9500.1646301626719302No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG6700.11610758841072973No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT6290.10900249717962537No Hit
GGGGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGAC6130.10622977865041391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA107500.0290.88371
GGGTTAT108300.0289.14362
ATTAGTA111350.0279.36917
GGGGGGA81800.0278.95171
GGTTATT112200.0278.56733
TTATTAG112350.0277.01385
TATTAGT113000.0275.550876
TAGTAGA114500.0271.296979
GTTATTA115300.0270.565924
TTAGTAG115500.0269.331158
GGAGCGA64300.0263.80255
GAGCGAA64750.0261.057926
GGGGGAG88900.0259.1622
CGAAAGC64900.0257.954539
GCGAAAG65400.0257.11018
GGGGAGC98250.0234.498733
AGCGAAA72550.0232.381127
GGGAGCG73250.0232.375434
GAGCAAA31550.0174.381946
GCAAAAG35150.0147.709818
Produced by FastQC (version 0.11.2)