FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_7098_flu.3520000003eaf4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_7098_flu.3520000003eaf4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576929
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT87921.5239310209748513No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT75021.3003333165779498No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA74681.294440043748884No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG72241.2521471446226486No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA60821.054202510187562No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA53600.9290571283468156No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT42920.7439390288926367No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC38390.6654198350230272No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT33460.5799673790015756No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC30020.5203413244957352No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG29550.5121947414673209No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC28250.48966163947383473No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA27460.47596844672394695No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG26990.46782186369553275No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA25360.4395688204267769No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT24540.42535563301550106No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA24530.425182301461705No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA24220.41980902329402753No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT22970.39814257906952155No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT22930.39744925285433735No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT21580.3740494930918709No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT21180.367116230940029No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT21060.36503625229447645No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA19090.3308899361966551No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC17720.3071435133265965No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA17410.30177023515891904No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA16600.2877303793014392No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT16520.2863437268710708No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15490.2684905768300779No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT15270.26467728264656487No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT15160.26277063555480834Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG14570.2525440738808415No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC12840.22255771507412522No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA12770.22134439419755292No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA11980.2076512014476651No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT11330.19638465045092204No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA10950.18979805140667222No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG9520.16501163921383738No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA9390.16275832901448878No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA8310.14403852120451563No Hit
GGGGGGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT7650.1325986386539765No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG7250.12566537650213458No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG5980.10365226917003652No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA5970.10347893761624048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT79050.0291.08161
CGGGTTA80550.0286.576662
TTATTAG88450.0261.147556
GTTATTA88850.0259.971865
GGGGGGA63700.0257.951321
GGGTTAT89800.0257.385863
TATTAGT92400.0250.62237
ATTAGTA92300.0247.697728
GGGGAGC68050.0240.81193
GGAGCAG68850.0234.15765
GAGCAGA69200.0231.907526
GGGAGCA70700.0231.368474
CAGAAGC70100.0230.402989
TTAGTAG99300.0230.236659
GGGGGAG72000.0228.829862
GGTTATT102200.0226.157034
GCAGAAG85100.0190.138088
AGCAGAA113650.0141.59487
CCGGGTA403.6111058E-4110.6251
CGGGCTA804.272806E-9110.6252
Produced by FastQC (version 0.11.2)