FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_7047_flu.3520000003e9f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_7047_flu.3520000003e9f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409479
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC147393.5994519865487606No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT118992.9058877256220708No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG90252.2040202305856957No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG82202.0074289524004896No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA80291.9607843137254901No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA71201.738794907675363No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT54091.3209468617438258No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT46551.1368104347231482No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA44101.0769783065798246No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA38100.9304506458206648No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA37970.9272758798375497No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT35290.8618268580317915No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT23520.5743884301759065No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG22800.5568051108848073No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG21020.5133352381929233No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14010.34214208787263817No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA11180.2730298745479011No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10790.2635055765985557No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA9960.24323591686020526No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9380.22907157632015315No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6720.164110980050259No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT6710.16386676728232705Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5990.1462834479912279No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG5100.12454851164528584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA65150.0291.151181
GGGTTAT65350.0290.485842
GTTATTA65650.0286.686984
GGTTATT65950.0286.277473
GGGGGGA44600.0282.763461
TTAGTAG67050.0279.161048
TTATTAG67400.0279.024485
TAGTAGA67350.0275.727549
GGGGAGC46350.0273.678533
GGAGCGA34750.0269.532385
TATTAGT69500.0269.532386
ATTAGTA69650.0268.74017
GGGAGCG35300.0265.75074
CGAAAGC35800.0258.3319
GAGCGAA38250.0242.94126
AGCGAAA38800.0239.877587
GCGAAAG39150.0236.979578
GGGGGAG55950.0227.247542
GGGAGCA14200.0211.901414
CAAAAGC18350.0152.72489
Produced by FastQC (version 0.11.2)