FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_6957_flu.3520000003ea2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_6957_flu.3520000003ea2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342076
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC122113.56967457524059No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT97872.861060115296016No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA72312.1138577392158466No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG71392.0869631310001284No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG54271.5864895520293736No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA48881.428922227809025No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT40971.1976870636934482No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT40591.1865784211695647No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA36091.0550287070709434No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT25590.7480793741741601No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA25580.7477870414761633No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA19600.5729720880739952No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19050.5568937896841638No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG18860.5513394684222219No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG18060.527952852582467No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11540.33735193348846454No Hit
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG9760.2853167132450099No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT8550.24994445678738056Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT7660.22392684666565324No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7070.206679217483834No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA6830.19966323273190753No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6160.1800769419661128No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5890.17218395912019552No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5380.15727499152235178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA48000.0291.31251
GGGTTAT48450.0290.737882
GTTATTA48750.0287.738464
GGGGGGA35050.0284.05851
GGTTATT49550.0283.39053
TTATTAG50500.0276.891085
TTAGTAG50850.0276.14558
GGGGAGC36200.0275.849463
TAGTAGA51350.0272.882179
TATTAGT51950.0270.582286
GGAGCGA28100.0268.754465
GGGAGCG28250.0268.371674
ATTAGTA52800.0265.667637
CGAAAGC28750.0262.678289
GCGAAAG31100.0243.303868
GAGCGAA31300.0241.277976
AGCGAAA31700.0238.698757
GGGGGAG43900.0228.137822
GGGAGCA12250.0195.061224
AAACGTT1450.0172.93103295
Produced by FastQC (version 0.11.2)