FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_6955_flu.3520000003ebd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_6955_flu.3520000003ebd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460526
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA165273.588722460838259No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC160523.4855795329688224No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC139083.020024928017093No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG119402.592687492128566No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG82301.7870869397167588No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA79411.7243326109709332No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC76761.6667897143700898No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG76681.6650525703217627No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT74011.6070753877088375No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA68791.4937267385554778No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG66841.451383852377499No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA59061.2824465936776643No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA45270.9830063883472376No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT38310.8318748561427586No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA33880.7356805044666317No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA31270.6790061798899519No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC17360.3769602584870344No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG16580.36002310401584275No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG15380.33396594329093254No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA10640.23104015842753722No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA9560.207588713775118No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT8830.19173727433413099No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT8260.17936012298979861Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA7390.1604686814642387No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG6690.14526867104137442No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA5190.11269722013523666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA97900.0291.68541
GGGTTAT99600.0287.003022
GGGGGGA61200.0282.467321
ATTAGTA101150.0280.56357
GGTTATT102400.0278.723143
TTATTAG102050.0278.378275
TTAGTAG102400.0276.418468
TATTAGT104700.0271.614146
GAGCGAA50400.0271.001986
GGAGCGA50600.0270.805335
TAGTAGA104500.0270.581339
GTTATTA105650.0269.17184
GCGAAAG50650.0269.081948
CGAAAGC50800.0268.287389
GGGGGAG67550.0256.132482
GGGAGCG60300.0227.976784
AGCGAAA60000.0226.904167
GGGGAGC76950.0224.077323
GCAAAAG21650.0146.478068
GAGCAAA24050.0133.087316
Produced by FastQC (version 0.11.2)