FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_6667_flu.3520000003eacd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_6667_flu.3520000003eacd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407330
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC184894.539071514496845No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT152963.7551862126531312No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG92002.258610954263128No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG89062.1864336042029806No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA70201.7234183585790392No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA68461.680701151400584No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT65291.6028772739547787No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT56731.3927282547320354No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA55711.3676871332825964No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA39140.9608916603245525No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT32160.7895318292293717No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG31100.7635087030172096No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT22560.553850686175828No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA21930.5383841111629392No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG17140.42078904082684804No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT11940.29312842167284514Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11850.2909189109567181No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA8470.20793950850661624No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG7840.19247293349372746No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7310.1794613703876464No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT6460.15859376917978052No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCCTTAATTGTCGTACTCT5410.13281614415829918No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT5330.13085213463285297No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4270.10482900842069084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA76950.0292.699831
GGGTTAT77250.0291.754062
GTTATTA77650.0289.301364
GGTTATT77950.0288.75563
TATTAGT78950.0285.098186
TTAGTAG78900.0284.904948
TTATTAG79700.0282.04525
TAGTAGA79750.0281.49849
GGGGGGA42300.0278.262421
GGGGAGC42950.0275.42493
ATTAGTA83250.0270.372387
GGAGCGA34000.0269.838235
GGGAGCG34100.0269.046944
CGAAAGC34300.0266.188059
GCGAAAG34300.0266.188058
GAGCGAA35450.0257.13686
AGCGAAA35850.0255.090677
GGGGGAG55000.0215.081822
GGGAGCA12850.0200.875494
AGACCCC1400.0158.0357295
Produced by FastQC (version 0.11.2)