FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_6628_flu.3520000003eb6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_6628_flu.3520000003eb6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500608
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC299585.984323063155203No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG151893.034110521605727No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT133482.666357709025825No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA132332.6433856430580414No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA93431.866330542060854No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG70231.40289408079775No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA62891.2562723727946816No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT58951.1775680772181027No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT56491.128427831756584No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG46620.9312675786243927No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT44640.8917156737407314No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA38040.7598759907951931No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG31180.6228426233699821No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA30850.6162506392227052No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT29900.5972737151623626No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG21000.41948990028125804No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC20630.4120988877524929No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT16380.3272021222193812No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG13400.267674507798517No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT12900.2576866530299156No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12630.2522932114548709No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC10830.2163369342879059No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT10330.20634907951930453Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA8020.16020519048836615No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7980.15940616210687802No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7250.14482389414472002No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT6840.1366338532344669No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6030.12045352850933266No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTG5170.10327441830733827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA97250.0292.42161
GGGTTAT98100.0289.43682
GTTATTA98600.0287.07154
GGTTATT99600.0285.225923
GGGGGGA69400.0280.760071
TTAGTAG101800.0279.641458
TTATTAG101400.0278.853555
TAGTAGA102500.0276.72449
GGGGAGC70950.0274.210723
TATTAGT104300.0272.51446
GGGAGCG55150.0270.394384
GGAGCGA55150.0269.859475
CGAAAGC54950.0269.499549
ATTAGTA106100.0267.891147
GCGAAAG56050.0263.947368
AGCGAAA58100.0255.395877
GAGCGAA58700.0252.534076
GGGGGAG80450.0242.563722
GGGAGCA21600.0210.324084
AAACGTT2850.0165.61403295
Produced by FastQC (version 0.11.2)