FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_6471_flu.3520000003ebe6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_6471_flu.3520000003ebe6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337805
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA189165.599680288924083No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC121793.605334438507423No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG80992.3975370406003464No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG79412.350764494308847No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG75992.24952265360193No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC72142.1355515756131496No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA71842.1266707123932447No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG53591.5864181998490254No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA51271.5177395242817602No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA43981.3019345480380693No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC34611.0245555868030374No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT33520.9922884504373826No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT32240.954396767365788No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA32140.9514364796258196No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT23290.6894510146386229No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG22070.6533355042110094No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA20840.616923965009399No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT19250.5698553899439025No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT16550.48992762096475784Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG14750.43664244164532795No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA12210.36145113305013243No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA10760.3185269608205918No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG9460.28004322020100353No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC9140.2705702994331049No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA8050.23830316306745017No Hit
GGGGGGAGCGAAAGCAGGTGAAGCATCGACAACCATCTCAATTTCATTAT7710.22823818475155785No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT7580.224389810689599No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC7020.2078121993457764No Hit
GGGGGGAGCGAAAGCAGGCCTTTACTACAAACATGGGGTTGACAAGGTTG6580.19478693328991578No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC6080.17998549459007415No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA5900.17465697665813118No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG5820.1722887464661565No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT5560.16459199834223887No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5460.16163171060227052No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA4970.14712630067642576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA73350.0293.793461
GGGTTAT74700.0288.483922
GGGGGGA55250.0284.855221
ATTAGTA75950.0281.793957
TTATTAG76500.0280.53925
TATTAGT77250.0277.624576
GGTTATT78300.0274.843573
TAGTAGA77600.0274.281569
GTTATTA79650.0269.444464
CGAAAGC46400.0269.251079
GGAGCGA46250.0269.167575
TTAGTAG79600.0269.05788
GAGCGAA46650.0266.85966
GCGAAAG47050.0264.904368
GGGGGAG60200.0262.65782
AGCGAAA50700.0245.251467
GGGAGCG51400.0243.34634
GGGGAGC65400.0241.999223
GAGCAAA19650.0162.888056
GCAAAAG20350.0154.385768
Produced by FastQC (version 0.11.2)