FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_6271_flu.3520000003ec01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_6271_flu.3520000003ec01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405906
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA5290113.032820406695148No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT223635.509403655033431No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG175864.332530191719266No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG137263.381571102669091No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG103102.539996945105517No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC96012.365325962168581No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT88352.176612319108365No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC54731.3483417342931614No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT50791.2512749257217188No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTG50291.2389568028065612No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT49281.2140741945179425No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGTATGCT40440.9962897813779545No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA36780.906121121639No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC30790.7585500091154109No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTGGCCAAATA30030.7398264622843712No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT27470.6767576729587639No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC24050.5925017122190852No Hit
GGGGGGAGCAAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG23040.5676191039304667No Hit
GGGGGGAGCAAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT20590.507260301646194No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT19800.4877976674402448Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGGACCAAAACCAAAACAAGAAACTAGCTG18040.44443787477888974No Hit
GGGGGGAGCGAAAGCAGGGGAGCGAACGGGATTAGATACCCCGGTAGTCC14530.3579646519144827No Hit
GGGGGGAGCGAAAGCAGGGTTTGTTGGGGTTTTCGCAGTCTTTGGCCTTG12850.3165757589195528No Hit
GGGGGGAGCGAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA12670.3121412346700961No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT12640.31140214729518656No Hit
GGGGGGAGCGAAAGCAGGGATACCGTCTTTGTTACGTTTAAAATCTACAA12170.29982311175493837No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGTCGCGGTTTGGTTGAATTTG11500.28331682704862704No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG11400.2808532024655955No Hit
GGGGGGAGCGAAAGCAGGAAAGGAACATCCAAGACATTCTCTCGCTTTGC11380.2803604775489892No Hit
GGGGGGAGCGAAAGCAGGGGGAGCGAACAGGATTAGATACCCTGGTAGTC11210.2761723157578356No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGCCGTGGTTTGGTTGAATTTG10050.2475942705946697No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC9860.24291338388690978No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA9360.2305952609717521No Hit
CGGGTTATTAGTAGAAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAA8910.21950895034811013No Hit
GGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC8320.20497356530822405No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAACATTCT8050.19832177893403893No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT7990.19684360418421998No Hit
CGGGTTATTAGTAGAAACAAGGAATGTGGAACAAATTTACTTCTAACAGA7790.1919163550181569No Hit
CGGGTTATTAGTAGAAACAAGGGCACCACGGTGACGTCCACCAATTTCAC7450.18354003143584968No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTAATATCCGTGGGAAATCTCATC7340.18083004439451497No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA6850.16875828393766043No Hit
GGGGGGAGCGAAAGCAGGTCTACATATGGGCCTTTTTTTAATGAACGAGC6680.16457012214650685No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCTGTAAACG6630.16333830985499107No Hit
GGGGGGAGCGAAAGCAGGGACGAGGCGAAAGTGACGGTACCTGCAGAAGA6520.16062832281365638No Hit
CGGGTTATTAGTAGAAACAAGGCCGTCTGAAAAAAACCTAAAAACCGAGT6370.15693288593910903No Hit
CGGGTTATTAGTAGAAACAAGGCCGCGTGGAGGTTAGACTACGGTCTAGC6330.15594743610589643No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC6280.15471562381438067No Hit
GGGGGGAGCAAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC6210.1529910866062586No Hit
GGGGGGAGCGAAAGCAGCATAACGCCTGCCGTATCTTTATCCAAGCGATG6160.15175927431474281No Hit
GGGGGGAGCAAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA6130.15102018693983335No Hit
GGGGGGAGCAAAAGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCAA5590.13771661419146305No Hit
GGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATA5440.13402117731691573No Hit
CGGGTTATTAGTAGAAACAAGGTCGATACAGCCCCAGGTTGTGTAGCCCC5430.1337748148586126No Hit
GGGGGGAGCGAAAGCAGGACTTAGTGATCCGGTGGTTCCGCATGGAAGGG5150.12687666602612427No Hit
GGGGGGAGCGAAAGCAGGTGACTTGATTGCCGTTTCAGGTATGGAAGATA5050.12441304144309273No Hit
GGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC5040.12416667898478959No Hit
CGGGTTATTAGTAGAAACAAGGACAATAGCACATGAAAAAAAATGCTTTG4900.12071760456854543No Hit
GGGGGGAGCGAAAGCAGGAGGTGAAAGTAAGTTGTTCAGCATTCCATTTC4840.11923942981872651No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGTCGTGGTTTGGTTGAATTTG4620.11381945573605712No Hit
GGGGGGAGCGAAAGCAGGTTTCATGATGTTATTGGTTATCCAAATCAGAT4390.10815311919508458No Hit
GGGGGGAGCAAAAGCAGGGATACCGTCTTTGTTACGTTTAAAATCTACAA4380.10790675673678142No Hit
GGGGGGAGCGAAAGCAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGA4340.1069213069035688No Hit
CGGGTTATTAGTAGAAACAAGGGATAAAGAGACATTTCCCCCTGCCCTAT4290.10568949461205303No Hit
GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT4120.10150133282089942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA110050.0286.288061
GGGTTAT110650.0284.602362
TATTAGT110700.0283.54116
GGTTATT111150.0282.79133
TTATTAG111050.0282.38185
GTTATTA111450.0281.235964
ATTAGTA112150.0279.612127
TAGTAGA113100.0277.39399
GGGGGGA151850.0277.224241
TTAGTAG114950.0272.801248
GGGGGAG161050.0264.50172
AGCGAAA121050.0260.638187
GGGGAGC164000.0259.564033
GCGAAAG121750.0259.503088
GGGAGCA42150.0258.606174
CGAAAGC122800.0258.36529
GGAGCGA124500.0253.771095
GGGAGCG124650.0253.46574
CAAAAGC42800.0251.232489
GCAAAAG43300.0249.6948
Produced by FastQC (version 0.11.2)