FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_30515_flu.3520000003eb2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_30515_flu.3520000003eb2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443842
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT126002.8388480585433555No Hit
CCGGGTTATTAGTAGAAACACGAGCAATTAGTATGGGTTAGCTTCACGCG119572.6939766853970557No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT61941.3955416567156782No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA39060.8800428981484402No Hit
CCGGGTTATTAGTAGAAACACGTGCAATTAGTATGGGTTAGCTTCACGCG37420.8430928123070823No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT35270.7946521509906679No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG31450.7085854876284804No Hit
CCGGGTTATTAGTAGAAACACGAGCGTGCCAAACAATTCCTGCAACAGGT30850.6950671635401787No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT29010.6536109696693868No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT27670.6234200458721797No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA24670.5558284254306713No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT24210.5454643769629732No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA22690.5112179559392757No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT17110.38549754191806995No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15380.3465197074634667No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT15080.3397605454193159No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG15040.3388593238134291No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC14970.33728218600312726No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA14790.33322668877663675No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT14040.3163287836662596No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG13890.3129492026441842No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT13670.30799248381180694No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT13530.3048382081912032No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT13410.30213454337354284No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA12530.2823076680440337No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC12150.27374606278810926No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT11980.26991587096309044No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11070.2494130794291662No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA10810.24355513899090214No Hit
GGGGGGAGCAGAAGCGGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT9920.2235029582599213No Hit
CCGGGTTATTAGTAGAAACACGTGCGTGCCAAACAATTCCTGCAACAGGT9490.21381482599663842No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCTGATACCTCCAGCACACTTTA9250.20840749636131775No Hit
CCGGGTTATTAGTAGAAACACGAGCGGTGTCCCTGGACCAGATGCTCGCC8650.19488917227301608No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA8580.19331203446271422No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT8480.19105898044799724No Hit
CCGGGTTATTAGTAGAAACACGAGCCAACCTACCCGAACAGCGAGCCACA8410.18948184263769538No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC8410.18948184263769538No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA8290.18677817782003506No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT8150.1836239021994313Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA8020.1806949319802993No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA7780.17528760234497862No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG7700.17348515913320506No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT7540.16988027270965794No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG7450.1678525240964127No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA7380.16627538628611083No Hit
GGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAATA7360.16582477548316743No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT6810.15343297840222422No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGCA6700.15095461898603557No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCAATTATTAAAGCAACA5630.12684694102856423No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTA5360.12076369518882846No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC5320.11986247358294168No Hit
GGGGGGAGCAGAAGCGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCC5190.11693350336380966No Hit
CCGGGTTATTAGTAGTAACAAGAGCAATTAGTATGGGTTAGCTTCACGCG5080.11445514394762099No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA4790.10792128730494185No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAGCCATGCAGCACCTGTC4520.10183804146520609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT82750.0290.365541
CGGGTTA83750.0287.426882
TTATTAG88350.0272.294866
GTTATTA89250.0270.04485
GGGGGGA68500.0267.222631
TATTAGT90600.0265.695347
GGGTTAT91600.0262.794743
TTAGTAG96050.0247.70179
GGGGAGC74450.0246.460713
GGGAGCA75150.0244.1654
GGTTATT98650.0244.014194
GGGGGAG76450.0240.977752
GAGCAGA76500.0238.699346
CAGAAGC76950.0236.920099
ATTAGTA108600.0218.941078
GCAGAAG84250.0216.566798
GGAGCAG84850.0214.861535
AGCAGAA87800.0206.80247
GCTAGGG1500.0117.99999295
GATCGGG2200.0100.56819295
Produced by FastQC (version 0.11.2)