FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_30427_flu.3520000003eba3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_30427_flu.3520000003eba3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396828
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC223105.6220831191347385No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT132643.34250607316016No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG109302.7543419315169295No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA91342.301752900500973No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA76451.9265273619805054No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG62361.5714616912113055No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT56361.420262683076799No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA55271.392794863265697No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG34780.8764502504863568No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT33360.8406664852278568No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA33210.8368865100244942No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT32740.8250425877206246No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA27980.7050913746005827No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG27470.6922394589091495No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC19210.48408882437731215No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG18130.4568730029131009No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT17570.44276109548721365No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17090.43066517483645306No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC12780.32205388732649914No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG12720.32054189724515403No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10690.2693862328263127No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT10170.25628231878798874Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA9410.2371304444242846No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7090.17866682794560867No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5190.13078714203634825No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4140.10432731561280958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA76050.0292.284671
GGGTTAT76550.0290.760932
GGGGGGA52400.0287.68131
GGTTATT77400.0287.377263
GTTATTA77200.0287.35754
TTATTAG77450.0285.85865
TTAGTAG78700.0281.693158
GGGGAGC54550.0276.883583
GGGAGCG40950.0275.909674
ATTAGTA80650.0275.065127
TATTAGT81050.0273.889566
GGAGCGA41150.0273.134865
TAGTAGA81800.0271.01779
CGAAAGC41750.0267.796429
GAGCGAA44300.0253.047416
GCGAAAG44900.0248.680398
GGGGGAG62250.0244.767042
AGCGAAA46750.0239.470587
GGGAGCA18500.0204.905414
AAACGTT2250.0183.55556295
Produced by FastQC (version 0.11.2)