FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_30301_flu.3520000003ea7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_30301_flu.3520000003ea7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405251
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC154993.8245433077278035No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT122203.0154151377788088No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG85552.1110373570947387No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA67601.6681019911116817No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG62401.5397864533338599No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA54821.3527418809577274No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT51401.2683497388038525No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA50281.2407125460517061No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT43101.0635383996584833No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG27150.6699551635899726No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA25860.63812303979509No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG23790.5870435853335341No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT23750.5860565427352431No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA23570.5816148510429339No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT20980.5177038428035958No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14000.3454649094018275No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT13440.3316463130257544No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9200.2270197976069152No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT9040.2230716272137515No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA8630.2129544405812694No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8270.20407105719665095No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7910.19518767381203256No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT7420.1830964019829686Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG6220.1534851240342405No Hit
GGGGGGAGCAAAACCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG4220.10413299411969372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA65500.0293.423651
GGGTTAT66450.0289.006742
GTTATTA66650.0286.36914
GGTTATT67250.0284.47213
GGGGGGA39750.0282.75471
TTAGTAG68150.0278.334568
TATTAGT68700.0276.750376
TAGTAGA68600.0276.293739
TTATTAG69000.0276.188425
GGGGAGC41250.0272.115143
GGAGCGA29250.0270.29065
GGGAGCG29550.0268.04574
ATTAGTA71100.0267.20117
GAGCGAA30600.0257.401986
AGCGAAA30900.0253.948237
CGAAAGC31050.0251.771329
GCGAAAG32000.0245.218758
GGGGGAG51800.0219.256762
GGGAGCA16200.0189.38274
AGACCCC1600.0175.15625295
Produced by FastQC (version 0.11.2)