FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_30295_flu.3520000003ea03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_30295_flu.3520000003ea03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383358
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC149983.912269993061316No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT103852.708956119345364No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG95122.4812316424856142No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG62961.6423291023012432No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA62831.6389380161624383No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA59771.559117065510567No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA55981.4602538619254064No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT45081.1759243318256043No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT41611.085408417197502No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT31050.8099478816145743No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA27470.7165625864074834No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG25690.6701307915838459No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG25620.6683048221244894No Hit
GGGGGGAGCGAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA24970.6513493914304644No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT23690.6179602356022308No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT13650.356064044574523No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT12600.32867450268417514No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9960.2598093687884432No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8920.23268067967800332No Hit
GGGGGGAGCAAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA7340.1914659404525274No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT7260.18937911821326278Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA7240.18885741265344666No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG6630.17294539307905404No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6160.1606853124233745No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC4580.11947057319789858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA54850.0291.7731
GGGTTAT55300.0289.93222
GGTTATT56600.0283.272983
GTTATTA56750.0282.00444
TTATTAG57600.0277.074685
TTAGTAG57650.0275.810948
TAGTAGA58700.0270.37489
GGGGAGC46150.0270.070433
TATTAGT59150.0270.06346
GGGAGCG34500.0267.210144
GGGGGGA46550.0265.848541
CGAAAGC34350.0265.37129
GGAGCGA34800.0264.906625
ATTAGTA60150.0264.592687
GCGAAAG36750.0248.040828
AGCGAAA37200.0245.040317
GAGCGAA38050.0241.504616
GGGGGAG57000.0220.473682
GGGAGCA15650.0198.865814
AAACGTT1450.0183.10344295
Produced by FastQC (version 0.11.2)