FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n02_30191_flu.3520000003ea60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n02_30191_flu.3520000003ea60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333322
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC109563.28691175499967No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT98782.963500759025807No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG68292.0487696581683776No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA64201.9260654862265316No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG57761.732858917203185No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA44601.3380454935467805No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT39381.1814401689657448No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT38541.1562393121366126No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT29610.8883302032269096No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA28550.8565291219901476No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA25710.771326225091653No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT23580.7074240524177822No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA18490.5547188604412551No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG18310.5493186768350123No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG15170.4551154739261135No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT10250.3075104553554821No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9320.2796095067232286No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT9000.2700091803121306Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7060.21180720144484913No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6220.18660634461571693No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5540.16620565099213372No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5380.16140548778658476No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4450.13350453915433125No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG3480.1044035497206905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA50150.0293.23531
GGGTTAT50400.0291.780732
GGTTATT51050.0288.354553
GTTATTA51100.0288.072424
TTATTAG53000.0276.632085
TTAGTAG53400.0276.217228
GGGGGGA33900.0274.550141
TATTAGT53850.0273.63516
ATTAGTA54450.0271.161627
GGGGAGC34700.0269.07063
GGAGCGA26600.0266.16545
GGGAGCG26600.0266.16544
CGAAAGC27050.0262.28289
TAGTAGA56850.0259.714179
GCGAAAG29550.0240.592218
AGCGAAA29700.0238.383837
GAGCGAA29900.0237.282616
GGGGGAG40750.0229.484652
GGGAGCA11650.0196.244634
AAACGTT1950.0189.10255295
Produced by FastQC (version 0.11.2)