FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_plasmids_flu.3510000003ec2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_plasmids_flu.3510000003ec2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536546
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGG474028.834657233489766No Hit
GGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAG161463.009248042106362No Hit
GGCCGCGCTCCCCTCGCGCACACTGCGGAGCGCACAAAGCCCCGCGCCGC49100.9151125905327782No Hit
GGCCGCCCGTGCCACACGCAGGGGCCGGCCCGTCTAGAATTGACCTGCAT21210.3953062738329985No Hit
GCCGGGAGGGCGTCCCCGGCCCGGCGCTGCTCCCGCGTGTGTCCTGGGGT8040.14984735698337143No Hit
ACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCT8010.14928822505432898No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT7890.14705169733815926TruSeq Adapter, Index 1 (97% over 36bp)
GGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCCCCGGGGCC6800.12673657058295096No Hit
CCTTGTTTCTACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAA5480.10213476570508401No Hit
CTACTAATAACCCGGCGGCCCAAAATGCCGACTCGGAGCGAAAGATATAC5480.10213476570508401No Hit
GGCCCAAAATGCCGACTCGGAGCGAAAGATATACCTCCCCCGGGGCCGGG5370.10008461529859508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT48300.0287.629738
GGGCGTC48600.0286.157389
GGCGGTG20000.0251.184958
GAGGGCG58150.0240.428387
GGGCGGT21800.0230.44497
GCCCGTG2150.0226.122995
CGGGGGC22650.0221.146444
CGCGCTC7200.0216.891544
GGGGGCG24000.0209.32085
GGAGGGC69050.0201.408396
CGGCCAT800.0184.1532295
CGCCCGT2700.0180.060914
GGCCGCG8700.0179.496441
CGTGCCA2850.0175.753228
GGGAGGG85100.0164.11495
CTCCCCT10050.0155.384988
CCGCGCT10200.0153.09993
GCCGGGA104800.0132.702762
CCCGTGC3750.0129.643846
CGGGAGG107700.0129.129524
Produced by FastQC (version 0.11.2)