FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_8033_flu.3510000003eb05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_8033_flu.3510000003eb05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505243
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT93621.8529697591060144No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG58761.1630047323763022No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCTACCT56321.114711138996483No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA43330.8576071316178552No Hit
GGGGGGAGCAGAAGCAGAGGATTGAAGCAGTACCGTAAGGAGTTGTGGAC41890.8291059945412407No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT39140.7746767397074279No Hit
CCGGGTTATTAGTAGAAACAACAGCTTCAACAACAGCTGCTACAGCAACA34230.6774957792586933No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT32360.6404838859717007No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT31040.6143578436514706No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA30470.6030761435586441No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT28980.5735853836668692No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA26890.5322191499931717No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG26020.5149997130093836No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCAATCGCGTGCTGACCCACCT24200.47897744253755126No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT24050.4760085740920706No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC23130.45779951429312227No Hit
CCGGGTTATTAGTAGAAACACGAGCATAGTAGTTTGTATTGTAACGGGTA22930.45384102303248136No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG22890.4530493247803532No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT20070.39723459800531624No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA18870.37348365044147075No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC18340.3629936486007723No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT17560.34755553268427275TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT17150.3394406255999588No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT16500.32657552900287584No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC16450.3255859061877156No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA15400.3048038270693508No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC14660.2901574094049794No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA14010.2772923128078964No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA13300.2632396688326212No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA13150.26027080038714046No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG13140.26007287582410843No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG12590.2491870248573459No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA11950.23651985282329493No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA11290.2234568316631799No Hit
CCGGGTTATTAGTAGAAACACGTGCATAGTAGTTTGTATTGTAACGGGTA9350.185059466434963No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA9100.1801113523591618No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT8190.16210021712324565No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT8170.16170436799718155No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7420.1468600257697781No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT7210.14270360994610515No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGATTGGTTTGGTGATGATAATG6560.12983851334902213No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT6060.11994228519741985No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA5720.11321285005433031No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA5630.11143152898704187No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA5350.10588964122214459No Hit
GGGGGGAGCAGAAGCAGAGGATCTTCTCAAAAAACTGAGGCAAATAGGCC5320.10529586753304845No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG5310.10509794297001641No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAGAAACAATCAAGTTCAG5110.10113945170937548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT80450.0288.418431
CGGGTTA82250.0282.106542
GGGGGGA47800.0258.584531
GTTATTA90550.0256.248055
TTATTAG90700.0255.49946
GGGTTAT93000.0249.972663
TATTAGT96000.0241.854117
ATTAGTA96700.0239.9518
GGGAGCA52900.0233.933334
GGAGCAG53550.0231.093815
GAGCAGA54000.0229.168016
GGGGAGC54550.0227.12753
TTAGTAG104450.0222.006049
GGTTATT106500.0218.009344
CAGAAGC58150.0212.812949
GGGGGAG58750.0211.141052
CCGGTTA304.695794E-7196.429721
CCGCCAA7500.0188.57254295
GCAGAAG66250.0186.793558
CGGGCTA255.5814235E-5176.786762
Produced by FastQC (version 0.11.2)