FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_7965_flu.3510000003e9eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_7965_flu.3510000003e9eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277472
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC101333.651900011532695No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT68442.4665551839464883No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG57842.0845346557490485No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA44691.6106129627493946No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA37951.367705570291777No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA31001.117229846615154No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT29591.0664139084304003No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT28081.0119940029985006No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG24960.8995502248875562No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT24900.8973878445392689No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG21680.7813400991811787No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT16310.5878070580094568No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG15630.5633000807288663No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG13290.47896724714565797No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA11680.42094337446661284No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA11370.4097710760004613No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11030.39751758736016607No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT10810.3895888594164456No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8900.32075308499596356No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT8520.30705800945681005TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT7220.2602064352439165No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA6690.24110540883404452No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6680.24074501210932997No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4500.16217852612155462No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA4410.15893495559912352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG206.21385E-8294.64426295
CGGGTTA39350.0292.77231
GGGTTAT39400.0292.40082
GGTTATT40100.0288.398683
GTTATTA40250.0287.32394
GGGGGGA28000.0286.225861
TTATTAG41700.0276.979745
GGGGAGC29100.0274.900053
TTAGTAG42100.0274.348088
TAGTAGA42400.0272.75449
GGAGCGA19400.0271.86255
GGGAGCG19500.0270.468324
TATTAGT43900.0263.099186
ATTAGTA44400.0260.136387
CGAAAGC20650.0256.832769
GCGAAAG21900.0242.846078
AGCGAAA21800.0242.608467
GAGCGAA21850.0242.053286
GGGGGAG35450.0227.320742
AGGTTAT202.2912625E-5220.98322
Produced by FastQC (version 0.11.2)