FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_7765_flu.3510000003ea4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_7765_flu.3510000003ea4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357397
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA133563.737020736044231No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC124383.480163515642269No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC75712.118372566081976No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG70771.9801509246020532No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA59021.6513848745232893No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG58601.6396332369885591No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG48701.362630352241345No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG34870.9756657162762978No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG30450.8519937212679457No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG29350.8212156229626998No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA28890.8083447818532332No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC26060.7291611289406459No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA17560.4913303693091996No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA17220.48181713892394173No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA15420.4314529780608119No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13070.3656997680450591No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT12800.35814514391558966No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT11520.3223306295240307TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA10990.3075011821587758No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA9210.25769662308301416No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA6620.18522819161884405No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4870.13626303522413452No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT4480.12535080037045637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA55950.0293.590271
GGGGGGA35700.0291.34221
GGGTTAT56400.0290.986542
ATTAGTA57100.0285.097267
GGTTATT58400.0280.516723
TATTAGT58350.0279.494726
TTATTAG58950.0277.149785
GTTATTA61500.0265.658234
TTAGTAG61400.0265.131168
CGAAAGC25050.0263.473639
GCGAAAG25100.0262.948768
GAGCGAA25100.0262.948766
GGGGGAG39700.0262.729982
GGAGCGA25200.0261.90535
TAGTAGA64300.0253.173459
GGGGAGC46000.0225.466313
GGGAGCG31100.0213.640214
AGCGAAA32850.0201.362157
GATACCT150.0028592057196.42899295
GACCCGG150.0028592057196.42899295
Produced by FastQC (version 0.11.2)