FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_7331_flu.3510000003eb49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_7331_flu.3510000003eb49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577051
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA225893.9145586785223485No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC186123.225364829105226No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG98601.70868779362656No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC98431.7057417801892727No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG92901.6099096960234018No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA88141.527421319779361No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG78471.3598451436701435No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG68101.180138323995626No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA47550.8240172879000297No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG43420.7524464908647589No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA41540.7198670481465244No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC41510.7193471634222972No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG29870.5176318904221637No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC28480.4935438981996392No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT26920.4665098925398275No Hit
GGGGGGAGCGAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT26220.45437924897452736No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG25240.4373963479831072No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA20440.35421479210676354No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT18920.32787396607925473TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC18860.3268341966308004No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA18790.32562113227427036No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13570.2351611902587466No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT12610.2185248790834779No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA10830.18767838544600043No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA10250.1776272807776089No Hit
GGGGGGAGCAAAAGCAGGGGAAAAACTAAAGCAACAAAAATGAAGGCAAT9210.15960461033773446No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTCAACTGTCATACTCCT7240.1254655134468184No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT7110.12321267964183408No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG7010.12147973056107692No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT6030.10449682956965675No Hit
GGGGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGAC5880.10189740594852101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA88700.0290.328031
GGGTTAT90000.0286.29812
GGGGGGA73450.0282.611821
ATTAGTA93150.0276.300177
GGTTATT94650.0272.699683
TTATTAG95250.0270.981875
TATTAGT95350.0270.388676
GTTATTA97100.0265.97074
TAGTAGA97150.0264.620679
GGAGCGA59000.0261.186555
GAGCGAA59300.0259.865176
GGGGGAG80350.0258.34272
TTAGTAG100500.0255.946568
CGAAAGC63550.0242.022679
GCGAAAG64250.0240.073758
GGGAGCG64900.0237.44234
GGGGAGC88150.0235.316013
AGCGAAA70050.0221.24777
TCGAACC20900.0154.37216295
CCCGTAA301.15428564E-4147.3232295
Produced by FastQC (version 0.11.2)