FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_6957_flu.3510000003ea20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_6957_flu.3510000003ea20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342076
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC107113.131175528245185No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT91652.6792291771419214No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG67621.9767537038552836No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA65051.9016242004700712No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG53571.566026263169588No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA48691.423367906547083No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT35611.0409967375670903No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT33680.9845765268536817No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA30820.9009693752265578No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG24650.720600100562448No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA24470.7153381119985033No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT20910.6112676715115939No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA16100.47065564377506747No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15940.4659783206071165No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14950.4370373835054199No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT13450.39318747880587934No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT9490.27742373039909257TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9460.2765467323051018No Hit
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG8510.24877512599539284No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA8050.23532782188753373No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7370.21544919842374208No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6920.20229422701387995No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA6420.18767759211403312No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5430.15873665501233644No Hit
GGGGGGAGCAAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG4000.11693307919877455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA47200.0293.082281
GGGTTAT47400.0291.845642
GGGGGGA31950.0287.265321
GGTTATT48300.0286.712523
GTTATTA48300.0286.407534
TTATTAG50650.0273.11915
TTAGTAG50750.0272.290688
TATTAGT50950.0271.22186
TAGTAGA50950.0271.22189
GGGGAGC33800.0269.798743
ATTAGTA51500.0268.325267
GGGAGCG23200.0267.337624
GGAGCGA23450.0265.115785
CGAAAGC24500.0253.152369
AGCGAAA26150.0237.17917
AAACGTT1250.0235.71431295
GAGCGAA26400.0234.933066
GCGAAAG27050.0229.28778
GGGGGAG40150.0227.862062
GGGAGCA13400.0217.683934
Produced by FastQC (version 0.11.2)