FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_6955_flu.3510000003ebdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_6955_flu.3510000003ebdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460526
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA145033.1492250166114397No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC138363.0043906315821474No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC115172.5008360005732575No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG94522.052435693098761No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG69851.5167438971958154No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA67541.4665838628003631No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG67251.4602867156251764No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG66391.4416124171056575No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT64881.4088238231934787No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC64321.3966638148551873No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA62091.3482409245080624No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA55521.2055779695391793No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT40320.8755206003569832No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA39220.851634869692482No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA29740.6457832999656914No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA29190.6338404346334409No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG15080.327451653109705No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT13590.2950973452096081TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTAGATAATCACTAAATGAGTGACATCGAAGC13400.2909716280948307No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11740.2549258890920382No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA9430.20476585469658606No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT8740.1897829872797627No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA7630.16568011360922077No Hit
GGGGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTG7040.15286867625280656No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG6590.14309724098096527No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA6440.13984009589035146No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4740.10292578486339532No Hit
GGGGAGCATGATATGAATGGTTCCCTTATGACAAACACATCCCCCTTGGA4720.10249149885131349No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA78400.0293.51911
GGGTTAT79550.0289.27592
GGGGGGA54900.0288.20621
ATTAGTA82600.0278.59447
GGTTATT83650.0275.273533
TTATTAG84300.0272.976265
TTAGTAG85150.0270.078288
GGAGCGA47950.0270.067085
CGAAAGC48100.0269.224889
GCGAAAG48150.0268.945348
GAGCGAA48350.0268.13756
GTTATTA86950.0264.656684
TAGTAGA87350.0263.27619
TATTAGT87600.0263.029276
GGGGGAG62550.0253.19352
GGGAGCG56800.0227.72864
GGGGAGC72600.0217.738143
AGCGAAA59850.0216.861857
GTTAACT354.0017767E-9210.46185295
AGCCCGG150.002859383196.43105295
Produced by FastQC (version 0.11.2)