FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_6929_flu.3510000003ec11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_6929_flu.3510000003ec11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445655
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC127092.8517575254400827No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT95352.139547407748146No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT90482.030270051946012No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG81701.8332566671528425No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG68081.5276390930203858No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA65241.4639126678708867No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA51701.1600902043060215No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA46981.0541786808181215No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT40560.9101210577689021No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT39040.876013956984663No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA37570.8430288003051688No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC36590.8210386958521726No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG33260.7463172184761755No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC29850.6698006305325869No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT25760.5780256027644702No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA24390.5472843342944654No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG24370.5468355566525676No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA23820.5344941715003758No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA22370.5019577924627795No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT21200.4757043004117535No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA20300.45550930652634886No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT19000.42633875980298663No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG18930.42476803805634405No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC18930.42476803805634405No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC18100.406143765917582No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT16900.3792171074037092TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14930.33501250967676793No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14850.33321739910917636No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT14540.3262613456597592No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG14480.3249150127340656No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG13210.2964176324735502No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG11380.2553544782398941No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9400.21092549169200392No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA8710.19544266304652702No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8350.18736466549236516No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8080.1813061673267438No Hit
GGGGGGAGCGAAAGCAGGGTCCGGTCGGTCTGCAGGCGGTCAGCCTCCTC8080.1813061673267438No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7610.1707598927421436No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT6810.15280878706622836No Hit
CGGGTTATTAGTAGAAACAAGGGCTCCACGGTGACGACCACCAATTTCTC6380.14316006776542392No Hit
GGGGGGAGCGAAAGCAGGCCCTGTCCCGTGGGGACTCCCTCCTCAAGCAG6080.13642840313695573No Hit
GGGGGGAGCGAAAGCAGGAAGTATTTCGAAGAAAATGGAAAGAAATCAAA5140.11533585396775532No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAACAACGCGAA4970.11152124401162333No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT4510.10119935824797208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA71650.0291.354371
GGGTTAT72650.0287.344022
GTTATTA73500.0284.22144
GGGGGGA76600.0282.91231
GGTTATT74500.0280.604133
TTATTAG75200.0277.60035
TTAGTAG75450.0276.094738
TAGTAGA76000.0274.290539
TATTAGT76650.0272.348886
ATTAGTA78000.0267.257427
GGGGAGC81700.0264.530643
GGGAGCG63850.0261.64964
GGAGCGA64400.0259.4155
CGAAAGC64500.0259.24129
GCGAAAG65800.0254.119458
AGCGAAA66450.0251.412027
GGGGGAG88200.0245.703892
GAGCGAA70700.0236.298846
GGGAGCA21750.0222.16854
TCGGACA150.0028594201196.42949295
Produced by FastQC (version 0.11.2)