FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_6777_flu.3510000003ea8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_6777_flu.3510000003ea8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344448
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC99222.880550910442215No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT81962.3794593088071347No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA52991.5384034745447788No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG52941.5369518766257897No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA52671.5291132478632479No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG48461.406888703084355No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA35501.0306345224823485No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT31940.9272807506503158No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT29090.8445396692679302No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG24310.7057669082125604No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT23070.6697672798216276No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA20370.5913809921962097No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA18250.5298332404310665No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15780.4581243032329989No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14450.4195117985878855No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT13740.3988991081382386No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT11540.33502879970271277No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT10150.29467437755481235No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA9330.27086817168338906No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA9030.26215858416945376No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8720.2531586770717206No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7950.2308040691192865No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT6870.1994495540691193TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG6020.1747723894463025No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG4300.1248374210330732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGT108.49309E-4294.644321
GCGTGTG108.49309E-4294.644322
CGGGTTA43350.0292.26541
GGGTTAT43550.0290.92322
GTTATTA44750.0284.109564
GGGGGGA29950.0283.822631
GGTTATT45350.0280.02583
TTATTAG46100.0275.789645
GGGGAGC31000.0273.258823
TATTAGT47100.0269.621436
TTAGTAG47100.0269.308658
GGAGCGA20650.0266.820745
GGGAGCG20650.0266.820744
TAGTAGA47700.0265.92119
ATTAGTA50750.0249.939657
CGAAAGC22450.0244.771339
GCGAAAG22800.0241.013878
GAGCGAA23850.0231.02096
AGCGAAA24150.0227.541057
GGGAGCA12800.0222.134194
Produced by FastQC (version 0.11.2)