FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_6617_flu.3510000003eb7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_6617_flu.3510000003eb7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513861
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC174143.3888541843027586No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT143542.7933624073436203No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG105752.0579495233146705No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA92331.7967894041384733No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA91281.7763558627722282No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT71371.38889699743705No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT60561.1785288239426615No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG60461.1765827723839715No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA53171.034715613755471No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT47920.932547906924246No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG44100.8582087373822883No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA36040.7013569817518746No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT31760.6180659750399428No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA27900.5429483848745089No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG23730.46179803487713605No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG23290.4532354080189No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT17510.3407536279266183No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT13870.2699173511903024No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG12900.2510406510710095No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC11230.21854159004088655No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT10140.19732962805116558TruSeq Adapter, Index 8 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9820.1911022630633576No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT9760.1899346321281436No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA8550.16638740826799464No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6690.13019084927636074No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTAGCCAAATA6510.12668795647071873No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC5840.11364941102749576No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC5840.11364941102749576No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT5350.1041137583899148No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG5310.10333533776643879No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT5270.10255691714296279No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGT108.4944675E-4294.6424295
CGGGTTA77550.0289.513241
GGGGGGA61000.0287.63861
GGGTTAT78500.0286.009582
GTTATTA79400.0282.396554
GGTTATT80150.0280.121673
TTAGTAG82400.0271.757578
TTATTAG82950.0270.310885
GGGGAGC65500.0268.10213
GGAGCGA50200.0266.469425
GGGAGCG50250.0266.204284
TATTAGT84350.0265.475076
ATTAGTA85450.0262.237
TAGTAGA86250.0259.456159
CGAAAGC51800.0257.10119
GCGAAAG54550.0244.148
AGCGAAA55200.0242.065817
GAGCGAA55650.0240.373146
GGGGGAG77750.0226.23992
GGGAGCA20250.0205.885934
Produced by FastQC (version 0.11.2)