FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_6271_flu.3510000003ec04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_6271_flu.3510000003ec04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405906
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATA4791611.804703552053923No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT247826.105354441668761No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG164114.043054303213059No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG157483.8797159933580683No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG156243.849167048528477No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT97972.4136130039959993No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC61081.5047818953156642No Hit
GGGGGGAGCGAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT59661.4697984262366164No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAAT58391.4385103940321158No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTG47791.1773661882307727No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA38550.9497272767586584No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC38370.9452927525092016No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGTATGCT36440.8977447980566929No Hit
GGGGGGAGCAAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG28280.6967130320813193No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC27650.6811921972082206No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTGACCGGCTTGGCCAAATA27110.6678886244598503No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT24300.598660773676664No Hit
GGGGGGAGCAAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT23510.5791981394707149No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCGTTGC22820.5621991298477973No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT19660.48434859302400063TruSeq Adapter, Index 1 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGACCAAAACCAAAACAAGAAACTAGCTG17180.4232507033648185No Hit
GGGGGGAGCAAAAGCAGGCAGTATTCCGTATGGCATTTAAAATGCACAAT14910.3673264253300025No Hit
GGGGGGAGCGAAAGCAGGGGAGCGAACGGGATTAGATACCCCGGTAGTCC14690.3619064512473331No Hit
GGGGGGAGCGAAAGCAGGGTTTGTTGGGGTTTTCGCAGTCTTTGGCCTTG13340.32864751937640735No Hit
GGGGGGAGCGAAAGCAGGGATACCGTCTTTGTTACGTTTAAAATCTACAA11610.2860268140899617No Hit
GGGGGGAGCGAAAGCAGGGGGAGCGAACAGGATTAGATACCCTGGTAGTC11560.28479500179844597No Hit
GGGGGGAGCGAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA11460.2823313772154144No Hit
GGGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATA11260.27740412804935133No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA10870.26779599217552835No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGCCGTGGTTTGGTTGAATTTG10840.2670569048006189No Hit
GGGGGGAGCGAAAGCAGGTGTTGAAGCCGGTCGCGGTTTGGTTGAATTTG10790.26582509250910313No Hit
GGGGAGCGAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC10570.2604051184264337No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT10380.25572423171867376No Hit
GGGGGGAGCGAAAGCAGGAAAGGAACATCCAAGACATTCTCTCGCTTTGC9820.24192793405369717No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAATATCGAAAGAATAAAAGA9250.22788527393041738No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC9160.225668011805689No Hit
GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT8770.216059875931866No Hit
CGGGTTATTAGTAGAAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAA8240.20300266564179884No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG7880.1941336171428853No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAACATTCT7690.18945273043512537No Hit
GGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGGC7400.1823082191443339No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCTGTAAACG7030.17319280818711721No Hit
CGGGTTATTAGTAGAAACAAGGAATGTGGAACAAATTTACTTCTAACAGA6920.17048282114578253No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTAATATCCGTGGGAAATCTCATC6730.1658019344380226No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT6580.16210649756347528No Hit
GGGGGGAGCGAAAGCAGGTCTACATATGGGCCTTTTTTTAATGAACGAGC6510.1603819603553532No Hit
CGGGTTATTAGTAGAAACAAGGGCACCACGGTGACGTCCACCAATTTCAC6480.15964287298044375No Hit
GGGGAGCAAAAGCAGGCAGTATTCTGTATGGCATTTAAAATGCACAATAC6330.15594743610589643No Hit
GGGGGGAGCAAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC6300.155208348730987No Hit
GGGGGGAGCGAAAGCAGAAGAGACTAAACCTGTGGTGTGTGATAGCGGTT6300.155208348730987No Hit
GGGGGGAGCGAAAGCAGCATAACGCCTGCCGTATCTTTATCCAAGCGATG6200.15274472414795542No Hit
GGGGGGAGCGAAAGCAGGGACGAGGCGAAAGTGACGGTACCTGCAGAAGA6190.15249836168965228No Hit
CGGGTTATTAGTAGAAACAAGGCCGTCTGAAAAAAACCTAAAAACCGAGT6160.15175927431474281No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC6090.15003473710662074No Hit
CGGGTTATTAGTAGAAACAAGGCCGCGTGGAGGTTAGACTACGGTCTAGC5690.1401802387744946No Hit
GGGGGGAGCGAAAGCAGGAGGTGAAAGTAAGTTGTTCAGCATTCCATTTC5590.13771661419146305No Hit
GGGGGGAGCGAAAGCAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGA5360.13205027765049052No Hit
GGGGGGAGCGAAAGCAGGACTTAGTGATCCGGTGGTTCCGCATGGAAGGG5340.1315575527338842No Hit
GGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAATAC5300.1305721029006716No Hit
GGGGGGAGCGAAAGCAGGTGACTTGATTGCCGTTTCAGGTATGGAAGATA5040.12416667898478959No Hit
GGGGGGAGCAAAAGCAGGTCAAGTACAATACCGTACAGATAAAGCCGGTA4950.1219494168600612No Hit
GGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTG4950.1219494168600612No Hit
CGGGTTATTAGTAGAAACAAGGTCGATACAGCCCCAGGTTGTGTAGCCCC4850.11948579227702966No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG4830.11899306736042334No Hit
GGGGGGAGCGAAAGCAGGTTTCATGATGTTATTGGTTATCCAAATCAGAT4630.11406581819436026No Hit
GGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAATA4550.11209491852793504No Hit
GGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGCTTGGCCAAATAA4340.1069213069035688No Hit
CGGGTTATTAGTAGAAACAAGGACAATAGCACATGAAAAAAAATGCTTTG4140.10199405773750572No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGAAAAAAAAAAATCGAA4140.10199405773750572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA150000.0284.91731
CGGGTTA99250.0282.02361
GGGTTAT100050.0279.768522
TATTAGT100900.0276.97376
GTTATTA101150.0276.726074
GGTTATT101250.0276.452763
TTATTAG101550.0275.636055
TAGTAGA102700.0271.688879
ATTAGTA102900.0271.447177
TTAGTAG105250.0265.246378
GGGGGAG161600.0264.92112
CCAGTCC20700.0262.6143295
GCGAAAG139050.0254.06258
AGCGAAA139050.0253.956547
GGGGAGC169100.0252.996963
CGAAAGC140100.0252.263539
GGGAGCG140300.0251.588934
GGAGCGA141500.0249.351175
GAGCGAA142950.0247.028026
GGGAGCA32250.0233.885134
Produced by FastQC (version 0.11.2)