FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_6205_flu.3510000003eaea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_6205_flu.3510000003eaea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770721
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT148471.9263780278466525No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG113241.4692735763006328No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT112691.462137401212631No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA79771.0350048850362192No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA73260.9505385217218683No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA69560.9025315256753091No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTCTATAT60710.7877039810774586No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT46710.6060558879283166No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA43470.5640173292280865No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC42690.55389693546692No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT38810.5035544639370149No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG38240.49615879157308546No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG38090.4942125620036304No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT35750.4638513807201309No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT35100.4554177192524922No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC35060.45489872470063747No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC34600.44893028735430845No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT34030.44153461499037916No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT33610.43608517219590487No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT33130.4298572375736486TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT32760.4250565379689927No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA29340.3806825037854165No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG25220.3272260649443832No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAACAAGA23540.30542829376648617No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC23170.30062759416183027No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA22220.28830147355528135No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT20610.26741194284312997No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC20230.2624814946005104No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA19840.25742129771992717No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT19660.25508582223658105No Hit
GGGGGGAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA19060.2473009039587607No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA19020.24678190940690597No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA18720.2428894502679958No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTCTATAT16820.21823720905489793No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA16160.20967379894929555No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA15960.20707882619002208No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT13340.17308468304353977No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG12420.16114780835088183No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA10280.13338159982665582No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG8600.11158382864875876No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGT108.495392E-4294.64124295
CCGGGTT106300.0292.285221
CGGGTTA108150.0287.285432
TTATTAG118050.0262.194526
GGGGGGA71800.0261.812131
GTTATTA119700.0258.457245
TATTAGT124200.0249.092837
ATTAGTA124550.0248.15638
GGGTTAT128750.0241.434193
GGGGAGC79500.0235.527683
GAGCAGA80000.0233.687326
GGAGCAG80650.0231.986595
CAGAAGC81100.0230.15449
GGGAGCA81350.0229.990394
TTAGTAG135850.0227.514659
GGGGGAG86150.0217.860092
GGTTATT147050.0211.187944
CCGGGTA304.6976493E-7196.427491
GAGACGC150.00285991196.42749295
GCAGAAG99600.0187.552758
Produced by FastQC (version 0.11.2)