FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30515_flu.3510000003eb2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30515_flu.3510000003eb2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443842
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCAATTAGTATGGGTTAGCTTCACGCG148233.339701966014933No Hit
GGGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT119122.6838379423308294No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT60851.3709833679552634No Hit
CCGGGTTATTAGTAGAAACACGTGCAATTAGTATGGGTTAGCTTCACGCG39220.8836477845719873No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA36960.8327287638393842No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT33900.7637853109890456No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG32650.7356221358050837No Hit
CCGGGTTATTAGTAGAAACACGAGCGTGCCAAACAATTCCTGCAACAGGT32210.7257086981403292No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT29910.6738884558018394No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA25840.5821891574028596No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA25220.5682202225116145No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT23760.535325633896747No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT19720.44430225170218235No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG14730.33187485636780656No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG13840.3118226756368257No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC13660.30776717841033524No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT13530.3048382081912032No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA12800.2883909138837694No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT12750.287264386876411No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT12520.282082362642562No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT12510.2818570572410903No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT12340.2780268654160715No Hit
CCGGGTTATTAGTAGAAACACGAGCCAACCTACCCGAACAGCGAGCCACA12180.27442197899252435No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA12140.27352075738663756No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT11800.2658603737366No Hit
GGGGGGAGCAGAAGCGGTGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT11450.2579746846850906No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCTGATACCTCCAGCACACTTTA10960.2469347200129776No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT10680.24062616877177012No Hit
GGGGGAGCAGAAGCAGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAATA10350.23319109052320422No Hit
CCGGGTTATTAGTAGAAACACGAGCGGTGTCCCTGGACCAGATGCTCGCC10320.23251517431878913No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT10160.22891028789524198TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCGGAGACGCCAGTCTCTGTGGAGCCGTCCTTGAAAT10090.22733315008494015No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCAGGCATAT9570.215617269208412No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA9140.20592913694512913No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT9040.20367608293041217No Hit
CCGGGTTATTAGTAGAAACACGTGCGTGCCAAACAATTCCTGCAACAGGT8960.20187363971863864No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC8290.18677817782003506No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA8140.18339859679795964No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGCA8040.18114554278324269No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG7950.17911779416999743No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7870.17731535095822387No Hit
GGGGGGAGCAGAAGCGGTGCGAATGGATGGGGGGTTGGGGTGATGTTGAG7490.16875374570229948No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA7420.16717660789199762No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA7400.16672599708905422No Hit
CCGGGTTATTAGTAGTAACAAGAGCAATTAGTATGGGTTAGCTTCACGCG7020.1581643918331298No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG7020.1581643918331298No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT6790.15298236759928083No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA6740.15185584059192236No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT6600.14870156497131862No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA6340.14284362453305455No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC5630.12684694102856423No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCAATTATTAAAGCAACA5470.1232420546050171No Hit
GGGGGGAGCAGAAGCGGAGCGCAGTTGCTCGACGGTCAGGTCGGGCATAT5060.11400453314467762No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTA5030.11332861694026253No Hit
CCGGGTTATTAGTAGAAACAACAGCAATTAGTATGGGTTAGCTTCACGCG4960.11175147912996065No Hit
GGGGGGAGCAGAAGCGGAGCATCACCAGCAGACCGGCCGCTGCGCAATAC4920.11085025752407389No Hit
GGGGGGAGCAGAAGCGGAGCGAATGGATGGGGGGTTGGGGTGATGTTGAG4900.11039964672113049No Hit
CCGGGTTATTAGTAGTAACAAGAGCGTGCCAAACAATTCCTGCAACAGGT4760.10724537110052676No Hit
GGGGGGAGCAGAAGCGGTGCTAACGTCCGTTGTCAAGAGGGAAACAACCC4710.1061188440931683No Hit
CCGGGTTATTAGTAGAAACACGAGCTGACGACAGCCATGCAGCACCTGTC4620.10409109547992303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT83000.0291.981871
CGGGTTA83800.0288.315462
GTTATTA89150.0270.352265
TTATTAG89350.0269.747136
GGGGGGA58650.0269.023071
TATTAGT92250.0261.267247
GGGTTAT93600.0257.971163
TTAGTAG99200.0242.665739
GGGAGCA65500.0239.53914
GGGGAGC66000.0238.170843
GGGGGAG66700.0235.67132
GGTTATT102350.0235.629094
GAGCAGA67150.0233.872576
CAGAAGC68250.0230.103189
GCTAGGG1200.0220.98326295
ATTAGTA113000.0213.29128
GCAGAAG74850.0209.813548
AGCAGAA78450.0199.809787
GGAGCAG82950.0189.325415
GATAGGG17050.0173.67596295
Produced by FastQC (version 0.11.2)