FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30455_flu.3510000003ebcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30455_flu.3510000003ebcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456872
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC159523.4915687544870337No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG150043.2840708119560835No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT101802.2281952056593535No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG85741.8766744295995377No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC73011.5980405890490115No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA72211.5805302141518849No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT66541.4564254320685004No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG47501.0396785095168888No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA46521.018228300267909No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA38870.8507853403141361No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT36610.8013185312297536No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC36570.8004430124848974No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT31340.6859689365949325No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG30790.6739305538531579No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT30510.6678019226391637No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA27680.6058589714405785No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA23170.5071442329580276No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA21100.4618361379117127No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA20270.4436691239559439No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT16490.3609326025670209No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT16070.3517396557460295TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT14070.30796371850321314No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13410.29351765921308376No Hit
CGGGTTATTAGTAGAAACAAGGTATCGAATTTAACCGGTTTAGCCAAATA12070.2641877812603968No Hit
GGGGGGAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG10520.2302614298972141No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10170.22260064087972123No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9690.2120944159414453No Hit
GGGGGGAGCGAAAGCAGGCGGTATTCCGTATGGCATTTAAAATGCACAAT9490.2077168222171637No Hit
GGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGT9090.1989616347686004No Hit
CGGGTTATTAGTAGAAACAAGGGAAGTCTCATCTTCAGGCGAGTTTCGCG8340.18254565830254427No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7850.17182055367805427No Hit
GGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCT7610.16656744120891628No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG7530.1648164037192036No Hit
GGGGGGAGCGAAAGCAGGACATAGTGATCCGGTGGTTCTGTATGGAAGGG6970.15255914129121503No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6390.1398641194907983No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGCCATGGC6140.13439212733544625No Hit
GGGGGGAGCGAAAGCAGGGGACCTTCGGGCCTTGCGTTATTCGAGCGGCC5730.1254180602006689No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4760.10418673063790294No Hit
GGGGGGAGCAAAAGCAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCC4600.10068465565847766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA64900.0291.468081
GGGTTAT65650.0288.362672
GTTATTA66750.0284.052034
GGTTATT67000.0282.992133
GGGGGGA80950.0280.996581
TTATTAG68400.0277.19995
TTAGTAG69300.0273.387338
TATTAGT71300.0266.751776
ATTAGTA71950.0264.137187
TAGTAGA72450.0261.297559
GGGGAGC88000.0257.98273
GGGAGCG63200.0255.251144
GGAGCGA63300.0255.080645
CGAAAGC64750.0248.685829
GGGGGAG95150.0239.061222
AGCGAAA67650.0238.460777
GCGAAAG67650.0238.2438
GAGCGAA69000.0233.795266
GGGAGCA29200.0225.525314
AAACGTT1800.0196.43071295
Produced by FastQC (version 0.11.2)