FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30429_flu.3510000003eb99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30429_flu.3510000003eb99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449694
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC216734.819499481869894No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG119232.651358479321494No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT117352.609552273323638No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA108822.419867732280173No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA76081.691817102296228No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG59291.3184521029855856No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG54021.2012613021298928No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT53061.179913452258647No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG46341.0304785031599266No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA40250.8950530805392111No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT35480.7889809514914586No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA34420.7654093672586247No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT32500.7227136675161332No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG30910.6873562911668823No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCAGCATTGC27690.6157520447237455No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC27300.6070794807135519No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA20460.4549760503809257No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18340.4078328819152579No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG17520.3895982601502355No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT16530.36758328997051326No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT16310.36269107437501946No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT13360.29709091070817045TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12310.2737416999114954No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC12130.2697389780606368No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA11820.2628454015397137No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA9840.21881546118026926No Hit
GGGGGGAGCGAAAGCATGGGTAGCAAACAGGATTAGATACCCTGGTAGTC8560.19035166135194154No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG7710.1714499192784427No Hit
CGGGTTATTAGTAGAAACAAGGACACCTACCTTAAGGATTTGGTGTGAGG7580.16855906460837813No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGTATTCACCGCGGCGTGCT7550.1678919442999017No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6490.14432036006706783No Hit
GGGGGGAGCGAAAGCAGGGGTAGCGAACAGGATTAGATACCCTGGTAGTC6050.13453592887608018No Hit
GGGGGGAGCGAAAGCAGGGGTAGCAAACAGGATTAGATACCCTGGTAGTC5980.13297931482296851No Hit
GGGGGGAGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACG5820.12942133984442755No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT5790.12875421953595112No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5760.1280870992274747No Hit
GGGGGGAGCGAAAGCAGGTGGATCATAAAGTCAGCAGTTCAAGACCAGCC5550.12341725706813966No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC5210.11585656023874014No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG5140.11429994618562846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA78800.0292.96011
GGGTTAT79400.0290.74632
GGGGGGA67300.0288.513461
GTTATTA80800.0286.073274
TTATTAG81400.0284.14565
GGTTATT81500.0283.435463
TTAGTAG82450.0280.169688
TATTAGT83650.0276.150486
TAGTAGA84000.0274.99999
ATTAGTA84450.0273.708957
GGGAGCG56050.0267.570284
GGGGAGC72750.0266.697243
GGAGCGA57200.0262.190835
CGAAAGC58350.0257.02349
GAGCGAA59600.0251.63286
GCGAAAG60600.0247.237358
AGCGAAA61450.0243.817447
GGGGGAG82400.0236.000232
GATACAA2200.0234.3749295
AAACGTT1550.0228.1105295
Produced by FastQC (version 0.11.2)