FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30337_flu.3510000003eb12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30337_flu.3510000003eb12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483994
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT93491.931635516142762No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG60921.2586932895862344No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT45580.9417472117422943No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT44510.919639499663219No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA42610.8803828146629916No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA38500.7954644065835527No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAA37590.7766625206097597No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT34790.7188105637673194No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT31490.650627900345872No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT31300.6467022318458493No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG28260.5838915358454857No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT26980.5574449270032273No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCAGAAACAATCAAGTTCAG24230.5006260408186879No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC24120.49835328537130624No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAACATAAATCCGTATTTTCT22150.45765030145001795No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC21430.4427740839762476No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA20840.4305838502130192No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA20030.41384810555502755No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC19860.4103356653181651No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT19120.3950462195812345No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT18610.38450889887064715TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC18490.38202952929168543No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA18380.37975677384430384No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA16450.3398802464493362No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA15270.3154997789228792No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG15170.31343363760707776No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA14960.30909474084389477No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT14140.292152382054323No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTGACAAGA13500.2789290776331938No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT13000.2685983710541866No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG12320.25454861010673685No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCAATAACGTTTCTTTGTAA12060.24917664268565312No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT11520.23801947958032535No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA10520.217358066422311No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT9420.19463051194849523No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAATAACGTTTCTTTGTAA7240.14958863126402394No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTT7140.1475224899482225No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT6160.12727430505336843No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5970.1233486365533457No Hit
GGGGGGAGCAGAAGCAGAGGATCTTCTCAAAAAACTGAGGCAAATAGGCC5000.10330706579007178No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCAGAAACAATCAAGTTCAG4850.10020785381636964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT63800.0291.873261
CGGGTTA65450.0284.292
GGGGGGA48550.0263.389431
TTATTAG73200.0253.385946
GTTATTA73300.0253.040255
TATTAGT76300.0243.28427
ATTAGTA76650.0242.173318
GGGGAGC52900.0241.173773
GGGTTAT78250.0237.786323
GAGCAGA53900.0236.4266
GGAGCAG54200.0235.117345
CAGAAGC54650.0232.911799
GGGAGCA55050.0231.754644
TTAGTAG83950.0221.114759
CCGGTTA202.2923152E-5220.983121
GGGGGAG58600.0218.217712
GGTTATT88350.0210.436314
GGGTATT150.0028594972196.429463
GCAGAAG65800.0193.891988
CGGTTAT255.5813114E-5176.78652
Produced by FastQC (version 0.11.2)