FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30304_flu.3510000003eb3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30304_flu.3510000003eb3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437185
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA156453.5785765751340968No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC152303.483651085924723No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA103762.373365966352917No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC96362.204101238606082No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG95222.1780253210883265No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG76751.7555497100769697No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG61211.4000937818086165No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG58331.334217779658497No Hit
GGGGGGAGCGAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA53161.2159612063542893No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT50061.1450530095954803No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA45091.0313711586628087No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC42900.9812779486944886No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA34580.7909694980385878No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA34190.7820487894140924No Hit
GGGGGGAGCGAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCG29690.6791175360545307No Hit
CGGGTTATTAGTAGAAACAAGGCCCGGGAACGCATTCACCGCGGCGTGCT29460.6738566053272642No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG27810.6361151457620916No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT27430.627423173256173No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA27040.6185024646316777No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG25770.5894529775724235No Hit
GGGGGGAGCAAAAGCAGGGGAAAACTAAAGCAACAAAAATGAAGGCAATA21820.4991022107345861No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC16250.3716961926873063No Hit
GGGGGGAGCGAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA14620.33441220535928723No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT14370.3286938023948672TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA10320.2360556743712616No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCCT9360.21409700698788842No Hit
GGGGGGAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC9010.2060912428377003No Hit
GGGGGGAGCAAAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAA8580.19625558973889773No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA8400.19213833960451523No Hit
CGGGTTATTAGTAGAAACAAGGGGTCTTACCGAAAGGAAAACTCATCTTG7460.1706371444582957No Hit
GGGGGGAGCGAAAGCAGGAGTGGACAGATTCTACAGGACATGCAAGTTAG6680.15279572720930498No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA6440.1473060603634617No Hit
GGGGGGAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCC6210.1420451296361952No Hit
GGGGGGAGCGAAAGCAGGCTTGACTGCGAGGCCTACAAGCCGAGCAGATG5740.13129453206308542No Hit
GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT5390.12328876791289729No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT4570.10453240618959939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCG108.4944745E-4294.6373295
CGGGTTA80600.0292.443971
GGGGGGA67200.0289.81441
GGGTTAT81950.0287.626432
ATTAGTA83550.0281.589327
GGTTATT84800.0277.438573
TTATTAG85600.0274.845675
TATTAGT85900.0273.885776
GTTATTA86900.0270.734074
TAGTAGA87450.0268.694439
TTAGTAG88300.0266.60848
GGAGCGA54350.0265.09235
GAGCGAA55000.0261.959356
GGGGGAG74900.0260.610442
CGAAAGC55900.0256.951149
GCGAAAG56250.0255.614238
GGGAGCG60250.0238.88854
GGGGAGC82950.0235.674333
AGCGAAA64350.0224.125817
GACCCGG202.2923768E-5220.97798295
Produced by FastQC (version 0.11.2)