FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30301_flu.3510000003ea70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30301_flu.3510000003ea70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405251
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC137463.3919718890268005No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT107602.6551445894026173No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG67211.6584783257783446No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA60261.4869796743252945No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG54901.3547159661543093No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA48111.1871654850944229No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT46471.1466967385644946No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA43471.0726685436926744No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT34090.84120705439345No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG28920.7136317985643466No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA20970.5174570821540231No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT20390.5031449644788045No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA19450.47994946341896755No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG18870.46563734574374893No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16440.4056745078975746No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT15780.38938830502577415No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG11740.28969700259838965No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT10830.26724178348727085No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT10080.24873473476931582TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT9260.22850036150435163No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9200.2270197976069152No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA8740.21566880772656946No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7780.191979785367587No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG5760.14213413415389475No Hit
GGGGGGAGCAAAAGCAGAAGAAGTGTCATTCAGGGGGAGGGGAGTTTTCG4390.1083279251624302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA57100.0293.61561
GGGTTAT57650.0290.81442
GGGGGGA35200.0285.021361
GTTATTA59100.0283.679354
GGTTATT59500.0281.772283
TTATTAG60200.0278.006445
AAACGTT850.0277.3154295
TATTAGT60450.0276.856666
TTAGTAG60650.0275.94378
TAGTAGA61450.0272.351269
GGAGCGA23800.0270.506325
GGGAGCG23850.0269.939244
GGGGAGC37100.0269.233283
GCGAAAG24850.0258.483648
ATTAGTA65450.0255.706447
CGAAAGC25150.0254.814549
AGCGAAA25900.0248.004587
GAGCGAA26150.0245.633596
GGGGGAG47250.0212.956972
GGGAGCA15850.0211.92324
Produced by FastQC (version 0.11.2)