FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30295_flu.3510000003ea06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30295_flu.3510000003ea06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383358
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC138283.6070722405688675No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT87692.287418027013914No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG81402.1233416284517346No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA55081.4367771117336798No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG52351.3655643028187752No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA51141.334001116449898No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA45961.198879376457515No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT37650.9821107163539042No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT36320.9474172966261302No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG31240.8149040844328277No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT28320.7387350726996698No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA23190.604917596606827No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG22300.5817016991950084No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT20470.5339656404718305No Hit
GGGGGGAGCGAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA20290.5292702904334852No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14690.38319273368496287No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12870.33571752774169317No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG11810.3080671330714371No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9580.2498969631519363No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA8880.23163726855837102No Hit
GGGGGGAGCAAAAGCAGGTACTGATACAAAATGGAAGATTTTGTGCGACA8610.224594243500853No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7440.19407446825160815No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT7160.18677059041418206TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG5840.15233802346631609No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC4540.11842716207826627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA51350.0293.493621
GGGTTAT51650.0291.78892
GGGGGGA39250.0287.13441
GGTTATT52500.0286.78413
GTTATTA52400.0286.76914
GGGGAGC40500.0276.81723
TTAGTAG54300.0275.64968
TTATTAG54500.0275.448975
GGGAGCG27150.0274.021734
GGAGCGA27250.0273.55685
TAGTAGA54800.0273.403359
TATTAGT55200.0271.689066
ATTAGTA55600.0269.999457
CGAAAGC28150.0263.764049
AAACGTT1300.0249.31177295
GAGCGAA31100.0239.218326
GCGAAAG31100.0238.744638
AGCGAAA31300.0237.689767
GGGGGAG47550.0236.704382
GGTATGC251.8919854E-7235.71295295
Produced by FastQC (version 0.11.2)