FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30291_flu.3510000003ea9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30291_flu.3510000003ea9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270941
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC99393.6683263145850944No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT79332.9279437220649513No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG62162.294226418297711No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA50601.8675652632861028No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG46971.733587755267752No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA46471.7151335530613674No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT35871.323904466286018No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA29951.1054067121624265No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA27921.0304826512045058No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT26890.9924669946593538No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT19520.7204520541372477No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA18520.6835436497244787No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT16400.6052978323694088No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG15560.574294772662683No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG10740.3963962633931372No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT9610.35468976640670846No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA8840.3262702950088765No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT8010.2956363193462784No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6720.24802447765380656No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6450.23805920846235898No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4840.17863667735780114No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4750.17531492096065196No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT3540.13065575162120166TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG2860.10555803662051885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATGC108.492393E-4294.6408295
GTAACGG108.492393E-4294.6408295
CGGGTTA44300.0292.312961
GGGGGGA24400.0292.22571
GGGTTAT44550.0290.67262
GGTTATT45150.0286.80983
GTTATTA45100.0286.801154
AAACGTT1000.0279.9088295
TTATTAG46600.0277.253235
TTAGTAG46600.0276.93718
TAGTAGA47100.0273.99729
ATTAGTA47250.0273.439187
TATTAGT47250.0273.127386
GGGGAGC26350.0270.59993
GGAGCGA18850.0264.94235
GGGAGCG18850.0264.94234
CGAAAGC19800.0250.742319
GAGCGAA20600.0242.435046
AGCGAAA20700.0241.263877
GCGAAAG21350.0233.91868
Produced by FastQC (version 0.11.2)