FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30191_flu.3510000003ea63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30191_flu.3510000003ea63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333322
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC96802.904098739357138No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT78042.3412796035065195No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA55081.6524561835102394No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG53801.6140548778658474No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG49521.485650512117412No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA38901.1670396793490978No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT34221.02663490558679No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT31490.9447321208921102No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA24740.7422252356580124No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT23810.7143242870257588No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG19700.5910200946832193No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19380.5814197682721212No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA19130.5739195132634509No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA14900.4470151985167496No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG12280.36841252602588487No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT11410.34231163859571223No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT11270.3381114957908569No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT8380.2514085478906283TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7350.22050749725490665No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7330.21990747685421305No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6200.1860063242150233No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5560.16680567139282734No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT4400.1320044881525972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA41650.0293.233341
GGGTTAT41950.0291.487522
GGGGGGA28400.0289.460721
GTTATTA42350.0288.73444
GGTTATT42550.0287.377233
GGGGAGC30050.0273.56693
TTATTAG44850.0272.96845
TTAGTAG45600.0267.18658
GGGAGCG22100.0266.649964
GGAGCGA22200.0265.448855
TATTAGT46350.0263.180946
ATTAGTA46550.0261.73377
CGAAAGC22900.0256.691359
TAGTAGA48150.0252.730459
GCGAAAG24650.0239.065498
GAGCGAA24900.0237.256886
AAACGTT1000.0235.71857295
AGCGAAA25200.0233.263157
GGGAGCA10450.0222.748414
GGGGGAG38650.0212.69562
Produced by FastQC (version 0.11.2)