FastQCFastQC Report
Fri 20 Nov 2015
000000000-AJTBG_l01n01_30082_flu.3510000003ea13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJTBG_l01n01_30082_flu.3510000003ea13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351357
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC109743.1233190174096435No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT97382.7715400575483056No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG56581.610327957035152No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA54231.5434444169320662No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTACTAATTG51071.4535074013040867No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA47961.3649934397208539No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT41761.1885347381722862No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA39461.123074252113947No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT35741.0171990311848063No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT27040.7695876273989133No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA26650.7584878058498906No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG26410.7516571464351073No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT21530.6127670716678478No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG15990.4550926835099343No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA15270.4346007052655846No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT14960.4257777701881562No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12970.36914021920724505No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGA9630.2740802090181781No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9080.25842661452596644No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT8760.24931906863958878No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8080.22996553363103622No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT7590.21601960399252043TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA5780.16450504757269674No Hit
GGGGGGAGCTGATGGATGATAATTATCGGGGAAGACTTTGTAATCCCCTG5550.15795899896686277No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC3630.10331372364859673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA49450.0293.75061
GGGTTAT49950.0291.10512
GGGGGGA30300.0288.809841
GTTATTA50500.0286.767734
GGTTATT51800.0280.139683
TTAGTAG52600.0275.318858
TTATTAG52850.0274.016485
TAGTAGA53250.0272.23489
TATTAGT54000.0267.90816
GGGGAGC32750.0266.304533
GGGAGCG21650.0264.703584
GGAGCGA21800.0262.206455
GCGAAAG22550.0253.485618
ATTAGTA57050.0253.327037
CGAAAGC22850.0250.157589
GAGCGAA23850.0239.66886
AGCGAAA24300.0235.230487
AGACCCC700.0231.50629295
GGGGGAG39100.0224.562712
GGGAGCA13550.0212.013474
Produced by FastQC (version 0.11.2)