##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJPTB l01n03.3330000003498a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20014396
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.348022143660994	31.0	23.0	33.0	12.0	34.0
2	28.32541926321434	32.0	23.0	34.0	11.0	34.0
3	27.620824730359086	32.0	23.0	34.0	11.0	34.0
4	26.07340731141724	32.0	19.0	34.0	11.0	34.0
5	25.69888739085606	31.0	19.0	34.0	11.0	34.0
6	23.90771412737112	31.0	11.0	37.0	2.0	38.0
7	26.682216190785873	34.0	21.0	37.0	2.0	38.0
8	27.103827764774916	29.0	21.0	37.0	11.0	38.0
9	29.542853254227605	32.0	25.0	37.0	11.0	38.0
10-14	28.69672592667798	33.2	22.2	36.8	10.8	37.8
15-19	30.871167433681233	35.6	26.6	37.6	11.0	38.0
20-24	30.12504320390183	34.0	25.6	37.0	11.0	38.0
25-29	30.92328339061543	35.0	27.0	37.2	11.0	38.0
30-34	27.64248382014626	31.4	19.4	36.8	11.0	38.0
35-39	29.137868602180152	32.2	24.0	36.6	14.2	37.8
40-44	26.80715903692522	29.6	15.8	36.0	11.0	37.2
45-49	26.405838737276905	29.4	18.2	35.6	10.6	37.2
50-54	27.27076463361672	31.4	18.8	35.8	11.0	37.0
55-59	30.01703330942388	34.2	24.0	37.0	11.0	37.8
60-64	30.299262390931005	34.8	25.6	37.6	11.0	38.0
65-69	27.361796578822567	30.0	19.2	36.2	11.0	37.6
70-74	24.130881651387334	25.2	11.0	33.8	11.0	37.0
75-79	28.223720436030145	32.0	22.2	36.4	11.0	37.2
80-84	26.023696783055556	28.8	15.0	36.0	10.8	37.4
85-89	28.72429817017711	33.2	23.0	37.0	11.0	38.0
90-94	24.899478145630773	27.0	14.2	35.0	10.2	37.2
95-99	25.93802695819549	28.0	16.2	35.2	11.0	37.0
100-104	27.260757176984008	30.6	18.8	36.4	10.8	37.8
105-109	24.613959142209435	26.8	15.0	34.8	10.0	36.6
110-114	24.105167320562657	26.0	12.6	34.0	11.0	37.2
115-119	22.00843907555342	21.8	11.0	31.4	10.8	36.6
120-124	23.472344116704797	24.0	11.0	32.8	11.0	36.8
125-129	25.34494062174047	27.8	13.8	35.2	11.0	37.0
130-134	24.43063872624485	25.8	12.4	34.0	10.0	37.0
135-139	22.34230999526541	22.2	11.0	31.8	10.4	36.6
140-144	21.586115194283156	20.8	10.6	30.8	10.2	36.2
145-149	19.432002444640347	17.8	9.8	27.4	9.2	35.0
150-154	19.968077277975315	18.8	9.6	27.8	9.6	35.4
155-159	19.847736529246248	18.0	9.8	28.4	9.8	35.0
160-164	19.37892557936797	17.8	9.6	26.8	8.8	35.2
165-169	18.57167024175998	16.8	9.6	26.2	8.6	34.6
170-174	17.572312929153597	15.6	9.2	24.6	8.4	33.2
175-179	19.276247217253022	17.8	10.2	27.2	9.0	34.4
180-184	15.952986280475315	15.0	8.8	23.0	6.6	28.0
185-189	2.0	2.0	2.0	2.0	2.0	2.0
190-194	2.0	2.0	2.0	2.0	2.0	2.0
195-199	2.0	2.0	2.0	2.0	2.0	2.0
200-204	2.0	2.0	2.0	2.0	2.0	2.0
205-209	2.0	2.0	2.0	2.0	2.0	2.0
210-214	2.0	2.0	2.0	2.0	2.0	2.0
215-219	2.0	2.0	2.0	2.0	2.0	2.0
220-224	2.0	2.0	2.0	2.0	2.0	2.0
225-229	2.0	2.0	2.0	2.0	2.0	2.0
230-234	2.0	2.0	2.0	2.0	2.0	2.0
235-239	2.0	2.0	2.0	2.0	2.0	2.0
240-244	2.0	2.0	2.0	2.0	2.0	2.0
245-249	2.0	2.0	2.0	2.0	2.0	2.0
250-254	2.0	2.0	2.0	2.0	2.0	2.0
255-259	2.0	2.0	2.0	2.0	2.0	2.0
260-264	2.0	2.0	2.0	2.0	2.0	2.0
265-269	2.0	2.0	2.0	2.0	2.0	2.0
270-274	2.0	2.0	2.0	2.0	2.0	2.0
275-279	2.0	2.0	2.0	2.0	2.0	2.0
280-284	2.0	2.0	2.0	2.0	2.0	2.0
285-289	2.0	2.0	2.0	2.0	2.0	2.0
290-294	2.0	2.0	2.0	2.0	2.0	2.0
295-299	2.0	2.0	2.0	2.0	2.0	2.0
300-301	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.16018028625642
1101	2	1.1178132634623132
1101	3	2.879326941458828
1101	4	4.753455868455756
1101	5	3.8527535193422615
1101	6	9.34593219555364
1101	7	6.208436389194983
1101	8	5.564502557394082
1101	9	3.6804307323997456
1101	10-14	4.995169240682355
1101	15-19	2.74231360633647
1101	20-24	3.780254081293986
1101	25-29	2.4415133040910035
1101	30-34	3.8634860603956227
1101	35-39	1.7644288176948457
1101	40-44	3.755420579053059
1101	45-49	2.911146694804284
1101	50-54	3.635787966715565
1101	55-59	3.122156449618789
1101	60-64	2.5532630884204828
1101	65-69	1.441210737819464
1101	70-74	3.3363124067816763
1101	75-79	2.6762499935642694
1101	80-84	3.2028718276982
1101	85-89	2.9450484860435147
1101	90-94	3.732594187164775
1101	95-99	3.4509892325265383
1101	100-104	2.4951096938674446
1101	105-109	2.616311889222196
1101	110-114	2.5158763466360305
1101	115-119	2.2139521634924755
1101	120-124	1.9293324531135383
1101	125-129	1.581251676200285
1101	130-134	1.1769041757615248
1101	135-139	1.667807727660577
1101	140-144	0.6080953279188606
1101	145-149	1.5427743410507837
1101	150-154	2.3175531489452794
1101	155-159	2.3558028684796177
1101	160-164	1.523492390718168
1101	165-169	0.18589380152684853
1101	170-174	0.5780250975995394
1101	175-179	0.08154202672536925
1101	180-184	0.3341981395502458
1101	185-189	0.0
1101	190-194	0.0
1101	195-199	0.0
1101	200-204	0.0
1101	205-209	0.0
1101	210-214	0.0
1101	215-219	0.0
1101	220-224	0.0
1101	225-229	0.0
1101	230-234	0.0
1101	235-239	0.0
1101	240-244	0.0
1101	245-249	0.0
1101	250-254	0.0
1101	255-259	0.0
1101	260-264	0.0
1101	265-269	0.0
1101	270-274	0.0
1101	275-279	0.0
1101	280-284	0.0
1101	285-289	0.0
1101	290-294	0.0
1101	295-299	0.0
1101	300-301	0.0
1102	1	1.2707136408690225
1102	2	1.2533569075146112
1102	3	1.7665249301205357
1102	4	3.139177825791439
1102	5	1.981858414503325
1102	6	8.23158707679681
1102	7	5.952629309169854
1102	8	3.473865550155441
1102	9	3.356566566075198
1102	10-14	3.6924575603684
1102	15-19	1.950951765255251
1102	20-24	2.3650579269827894
1102	25-29	1.502812466728912
1102	30-34	1.3354463051475918
1102	35-39	0.7099647810413039
1102	40-44	1.6101332952113694
1102	45-49	1.3308351395919829
1102	50-54	1.6543853856371165
1102	55-59	1.984177001978452
1102	60-64	1.8339970026433967
1102	65-69	1.0872625750476992
1102	70-74	1.639342736195946
1102	75-79	1.35322564725017
1102	80-84	1.4714744697246296
1102	85-89	1.5945817706205183
1102	90-94	1.5791631628852976
1102	95-99	1.9193729334123404
1102	100-104	1.613604993503202
1102	105-109	1.4639785053606182
1102	110-114	1.3821780157806849
1102	115-119	1.0917639609578345
1102	120-124	1.0105674353255658
1102	125-129	0.5052411673104835
1102	130-134	0.31629079277042393
1102	135-139	0.7332768995254746
1102	140-144	-0.08688048556983219
1102	145-149	-1.4252779128086175
1102	150-154	0.9856892597134959
1102	155-159	1.2320466882210077
1102	160-164	0.7920636785137845
1102	165-169	-0.272545333535696
1102	170-174	0.08201356302406637
1102	175-179	-0.17800899129849057
1102	180-184	0.19902410213180133
1102	185-189	0.0
1102	190-194	0.0
1102	195-199	0.0
1102	200-204	0.0
1102	205-209	0.0
1102	210-214	0.0
1102	215-219	0.0
1102	220-224	0.0
1102	225-229	0.0
1102	230-234	0.0
1102	235-239	0.0
1102	240-244	0.0
1102	245-249	0.0
1102	250-254	0.0
1102	255-259	0.0
1102	260-264	0.0
1102	265-269	0.0
1102	270-274	0.0
1102	275-279	0.0
1102	280-284	0.0
1102	285-289	0.0
1102	290-294	0.0
1102	295-299	0.0
1102	300-301	0.0
1103	1	0.4804671590459222
1103	2	1.0303621514974708
1103	3	0.9402973149988831
1103	4	2.343765030431353
1103	5	0.9023697183980524
1103	6	7.547990003574672
1103	7	5.749385796493652
1103	8	2.709625353671118
1103	9	2.7142950825906205
1103	10-14	3.4372710980460077
1103	15-19	1.6890325644791737
1103	20-24	0.9291336699243828
1103	25-29	1.3242970546494135
1103	30-34	0.7113142158751202
1103	35-39	0.30075380827086917
1103	40-44	0.5192959598738938
1103	45-49	0.5852405625844632
1103	50-54	0.7667414434336983
1103	55-59	1.3189698887057943
1103	60-64	1.2078179034199898
1103	65-69	1.0127151376888612
1103	70-74	0.7810558732820247
1103	75-79	1.3098246283549209
1103	80-84	0.888600852592937
1103	85-89	1.1638960308765967
1103	90-94	0.2701907795925962
1103	95-99	1.041385906998297
1103	100-104	0.6193838478664695
1103	105-109	-0.7598920592095268
1103	110-114	0.374670145534985
1103	115-119	0.40395236077734964
1103	120-124	0.42865217877597317
1103	125-129	0.3638004860155952
1103	130-134	0.07169163699593994
1103	135-139	0.37474536853472173
1103	140-144	0.19991734688074558
1103	145-149	0.28149812805441954
1103	150-154	0.6107939761308749
1103	155-159	0.7470381266078334
1103	160-164	0.4031707661584285
1103	165-169	-0.48498927706136996
1103	170-174	-0.37465127576560775
1103	175-179	-0.34362896217322003
1103	180-184	0.04741138906342712
1103	185-189	0.0
1103	190-194	0.0
1103	195-199	0.0
1103	200-204	0.0
1103	205-209	0.0
1103	210-214	0.0
1103	215-219	0.0
1103	220-224	0.0
1103	225-229	0.0
1103	230-234	0.0
1103	235-239	0.0
1103	240-244	0.0
1103	245-249	0.0
1103	250-254	0.0
1103	255-259	0.0
1103	260-264	0.0
1103	265-269	0.0
1103	270-274	0.0
1103	275-279	0.0
1103	280-284	0.0
1103	285-289	0.0
1103	290-294	0.0
1103	295-299	0.0
1103	300-301	0.0
2116	1	0.41042822270667045
2116	2	0.6911872239904362
2116	3	0.3996656884895522
2116	4	1.4883570170403004
2116	5	0.963580547419884
2116	6	7.0041541868691475
2116	7	5.260104283295803
2116	8	1.4487318652676606
2116	9	2.0794037811233608
2116	10-14	3.1151251126339297
2116	15-19	1.2438356676805533
2116	20-24	0.2695981701417871
2116	25-29	0.6383597393547618
2116	30-34	-0.43285864403460295
2116	35-39	-0.19989892933689646
2116	40-44	0.1937938353673765
2116	45-49	-0.08421028790382579
2116	50-54	-0.8307174443741765
2116	55-59	0.7227914645140743
2116	60-64	0.9638402067156022
2116	65-69	0.883195741690102
2116	70-74	0.21649471817924493
2116	75-79	0.6188062192073183
2116	80-84	0.7637091409448011
2116	85-89	1.3848723105656653
2116	90-94	0.6191646476938075
2116	95-99	0.5865468039473782
2116	100-104	1.1957535845345824
2116	105-109	0.421104417922912
2116	110-114	0.4735660297593256
2116	115-119	0.08115150755087441
2116	120-124	-0.027059337799041572
2116	125-129	0.2505024032450862
2116	130-134	-0.20938544522196878
2116	135-139	-0.16791440381976486
2116	140-144	0.39135097880918934
2116	145-149	-0.05550268275310799
2116	150-154	0.41120900423425155
2116	155-159	0.4414415646637835
2116	160-164	0.28837878930701777
2116	165-169	-0.24732266086674315
2116	170-174	-0.23763793418476808
2116	175-179	0.18492699259656575
2116	180-184	0.4968570942833743
2116	185-189	0.0
2116	190-194	0.0
2116	195-199	0.0
2116	200-204	0.0
2116	205-209	0.0
2116	210-214	0.0
2116	215-219	0.0
2116	220-224	0.0
2116	225-229	0.0
2116	230-234	0.0
2116	235-239	0.0
2116	240-244	0.0
2116	245-249	0.0
2116	250-254	0.0
2116	255-259	0.0
2116	260-264	0.0
2116	265-269	0.0
2116	270-274	0.0
2116	275-279	0.0
2116	280-284	0.0
2116	285-289	0.0
2116	290-294	0.0
2116	295-299	0.0
2116	300-301	0.0
1117	1	0.018650984955442595
1117	2	0.40218273334938814
1117	3	0.037818821402126446
1117	4	0.9251921027471681
1117	5	-0.3190747662140545
1117	6	7.051400954475465
1117	7	4.834003159758698
1117	8	1.1678645863214996
1117	9	1.3013161426709345
1117	10-14	2.4511356319523294
1117	15-19	0.908215716040889
1117	20-24	-0.20411154894988215
1117	25-29	0.21976810681143633
1117	30-34	-1.1610398501232169
1117	35-39	-0.6651389797005152
1117	40-44	-0.3765055489357856
1117	45-49	-0.972419735496473
1117	50-54	-0.8328834436624533
1117	55-59	-0.17608317690279662
1117	60-64	-0.6418409741920499
1117	65-69	0.0269224169796054
1117	70-74	-0.07405763190271486
1117	75-79	-0.3324756615057787
1117	80-84	-0.607549495074565
1117	85-89	1.0644857319503451
1117	90-94	-0.377581160726411
1117	95-99	0.07004062882336015
1117	100-104	0.6020579103453549
1117	105-109	-0.34370595018874184
1117	110-114	0.1914536451312756
1117	115-119	-0.21355395645440822
1117	120-124	-0.5060813324678293
1117	125-129	-0.16144590865646435
1117	130-134	-0.5456041306134409
1117	135-139	-0.7147646209941918
1117	140-144	-0.1456599186632559
1117	145-149	-0.24604378394392512
1117	150-154	0.0805882344825335
1117	155-159	-0.3885213456127481
1117	160-164	-0.31020986205043144
1117	165-169	-0.6401181592709833
1117	170-174	-0.42772208465712325
1117	175-179	-0.46552432380499553
1117	180-184	0.1265305670102883
1117	185-189	0.0
1117	190-194	0.0
1117	195-199	0.0
1117	200-204	0.0
1117	205-209	0.0
1117	210-214	0.0
1117	215-219	0.0
1117	220-224	0.0
1117	225-229	0.0
1117	230-234	0.0
1117	235-239	0.0
1117	240-244	0.0
1117	245-249	0.0
1117	250-254	0.0
1117	255-259	0.0
1117	260-264	0.0
1117	265-269	0.0
1117	270-274	0.0
1117	275-279	0.0
1117	280-284	0.0
1117	285-289	0.0
1117	290-294	0.0
1117	295-299	0.0
1117	300-301	0.0
2117	1	-0.002476352655595093
2117	2	0.12605582019474681
2117	3	-0.12762294346572034
2117	4	0.7104503498665302
2117	5	-0.19665221415163714
2117	6	6.465874036258718
2117	7	4.4798015411210805
2117	8	0.7823377679794099
2117	9	1.1951760398994615
2117	10-14	2.4684739126947868
2117	15-19	0.9774377670986496
2117	20-24	-0.6013408630609618
2117	25-29	-0.5285820322194184
2117	30-34	-1.6249706129997215
2117	35-39	-1.2382209161775002
2117	40-44	-0.5689567066019663
2117	45-49	-0.056754311027845006
2117	50-54	-1.1922661455493966
2117	55-59	-0.15278758694448769
2117	60-64	0.18721144620900176
2117	65-69	0.24006639167298616
2117	70-74	-0.32442872151953495
2117	75-79	0.07147803299806199
2117	80-84	-0.19798788868285655
2117	85-89	0.8699633994486362
2117	90-94	-0.1584643790433482
2117	95-99	-0.4514366897581752
2117	100-104	0.3985109724324971
2117	105-109	-0.22842587531102865
2117	110-114	0.1352136165695086
2117	115-119	-0.2602055076242671
2117	120-124	-0.28837603806146817
2117	125-129	-0.19600046824161765
2117	130-134	-0.2533629297235436
2117	135-139	-0.15468654849705743
2117	140-144	0.004871687743126074
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2110	300-301	0.0
2109	1	-0.811761544367922
2109	2	-0.47716913563066754
2109	3	0.16606162637422273
2109	4	0.5572250061010102
2109	5	0.311568944735523
2109	6	5.254449065873192
2109	7	-24.679613512315196
2109	8	-4.146979634270412
2109	9	-4.521595166389815
2109	10-14	0.013909702698622795
2109	15-19	-0.2470484749770776
2109	20-24	1.052217823073729
2109	25-29	0.07652058674263884
2109	30-34	0.788378869180498
2109	35-39	0.44708200388029695
2109	40-44	0.35532170587229217
2109	45-49	0.23804716114571534
2109	50-54	1.0962113491927
2109	55-59	-0.3749948205866964
2109	60-64	-0.8248595764527415
2109	65-69	-2.7932395979691726
2109	70-74	-1.388580887820087
2109	75-79	-0.9831499684134393
2109	80-84	-0.15446896182707803
2109	85-89	-2.668070891985689
2109	90-94	-0.7353150302723179
2109	95-99	-0.018060997504957044
2109	100-104	-0.3463379354772691
2109	105-109	0.5193856680987174
2109	110-114	-0.3582979479363573
2109	115-119	0.012102035840491965
2109	120-124	-0.47384794077879633
2109	125-129	-0.007835681689854823
2109	130-134	-0.4134403983754069
2109	135-139	-0.13780198880686
2109	140-144	0.09689760732348418
2109	145-149	0.5430580953075435
2109	150-154	-0.20259486327756093
2109	155-159	-0.4068939465906247
2109	160-164	-0.06020257175313759
2109	165-169	0.10479940707297786
2109	170-174	-0.10498617914685937
2109	175-179	-6.3684911497813825
2109	180-184	-4.1018385553182775
2109	185-189	0.0
2109	190-194	0.0
2109	195-199	0.0
2109	200-204	0.0
2109	205-209	0.0
2109	210-214	0.0
2109	215-219	0.0
2109	220-224	0.0
2109	225-229	0.0
2109	230-234	0.0
2109	235-239	0.0
2109	240-244	0.0
2109	245-249	0.0
2109	250-254	0.0
2109	255-259	0.0
2109	260-264	0.0
2109	265-269	0.0
2109	270-274	0.0
2109	275-279	0.0
2109	280-284	0.0
2109	285-289	0.0
2109	290-294	0.0
2109	295-299	0.0
2109	300-301	0.0
2108	1	-0.40975983758100654
2108	2	0.36479408218639264
2108	3	-0.2881685820766613
2108	4	1.0333824223581267
2108	5	1.204634025701374
2108	6	-21.892548357534306
2108	7	-2.3010900727625945
2108	8	-1.6062206967772177
2108	9	-1.5501692446407276
2108	10-14	-7.109637543491626
2108	15-19	-1.3498493535602272
2108	20-24	0.932489296793328
2108	25-29	-0.02521176716745188
2108	30-34	1.0502744593706943
2108	35-39	0.33182586873112996
2108	40-44	0.3290747267043237
2108	45-49	0.1775238786173361
2108	50-54	0.9039426952792873
2108	55-59	-0.41798636669462397
2108	60-64	-0.4618702176831917
2108	65-69	-2.736592827444376
2108	70-74	-1.848748009504824
2108	75-79	-0.7968786214858561
2108	80-84	-0.34951931507076495
2108	85-89	-2.6772143655902063
2108	90-94	-0.7058153833015126
2108	95-99	0.27980720114875623
2108	100-104	-0.628082080752506
2108	105-109	-0.18479281435892503
2108	110-114	-0.16278971964262823
2108	115-119	0.0767070944734094
2108	120-124	-0.3637344756481653
2108	125-129	0.1921978688729773
2108	130-134	-0.37774034544864676
2108	135-139	-0.3015399178398681
2108	140-144	-0.11771142788079914
2108	145-149	0.45199799946081853
2108	150-154	0.11792574073439255
2108	155-159	0.11211979710033759
2108	160-164	0.5328577616946717
2108	165-169	0.08021455829013036
2108	170-174	-0.24055913165186382
2108	175-179	-0.11601551804395172
2108	180-184	-3.1735612182984845
2108	185-189	0.0
2108	190-194	0.0
2108	195-199	0.0
2108	200-204	0.0
2108	205-209	0.0
2108	210-214	0.0
2108	215-219	0.0
2108	220-224	0.0
2108	225-229	0.0
2108	230-234	0.0
2108	235-239	0.0
2108	240-244	0.0
2108	245-249	0.0
2108	250-254	0.0
2108	255-259	0.0
2108	260-264	0.0
2108	265-269	0.0
2108	270-274	0.0
2108	275-279	0.0
2108	280-284	0.0
2108	285-289	0.0
2108	290-294	0.0
2108	295-299	0.0
2108	300-301	0.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	12.0
3	0.0
4	0.0
5	0.0
6	0.0
7	425.0
8	15056.0
9	111580.0
10	313223.0
11	771635.0
12	1438084.0
13	2096490.0
14	2947073.0
15	3286130.0
16	2701339.0
17	1845513.0
18	1214627.0
19	909226.0
20	1021201.0
21	1079905.0
22	261422.0
23	1455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.372840852858625	13.195125066052496	64.0352208691509	17.396813211937975
2	82.56346535571618	6.432129012813983	6.341559150658846	4.662846480811
3	6.695939280469486	76.53383686452703	4.344805216088589	12.425418638914893
4	6.9535295318534365	75.07483246133366	4.561347749869664	13.410290256943238
5	25.053001881047148	60.02358104051566	4.979698600766329	9.943718477670858
6	5.763777625520347	81.53698861087659	5.035881836601488	7.6633519270015755
7	6.62237315530634	9.065202954271202	5.55641911513613	78.75600477528633
8	8.846938942578655	72.29617171696279	4.788529537314967	14.06835980314359
9	6.170856919703349	6.067226450736631	79.97483210075313	7.787084528806883
10-14	22.173119687360057	8.402460298846956	43.39235755489856	26.03206245889443
15-19	21.208380145083456	49.38662962983652	7.047966173207891	22.357024051872134
20-24	22.008804267970476	6.408645901867377	45.65926585599637	25.923283974165777
25-29	20.627151952315895	15.344312370543106	27.54014612690553	36.488389550235475
30-34	30.62555210292757	16.02915683040757	42.925145235546594	10.42014583111826
35-39	22.934886229823512	14.49700275561816	33.856479420001136	28.711631594557197
40-44	13.050594012786004	20.265546019302917	43.45637817281811	23.22748179509297
45-49	17.547426890580294	41.93512725647714	23.718498655766773	16.798947197175792
50-54	18.812391128300526	34.92174727935669	13.171545024818151	33.094316567524636
55-59	6.582029204596054	33.823035472887895	51.593662837687134	8.001272484828911
60-64	5.3258546453390725	24.794116071721216	19.507038537883552	50.37299074505616
65-69	14.51882705957875	18.733634769873504	21.251323048463544	45.4962151220842
70-74	16.954062638150642	29.03735633332507	26.11185535362967	27.89672567489462
75-79	11.917735764438216	43.85334567379141	20.97244661639349	23.256471945376887
80-84	27.088884944836522	30.257612028005575	29.188164164214736	13.46533886294316
85-89	5.320356599533616	13.058616243197893	5.507991652403591	76.1130355048649
90-94	22.306935025598143	28.616189374429663	24.94376987266626	24.13310572730593
95-99	5.456372776698998	18.625644436521256	35.68927827106745	40.2287045157123
100-104	5.094326160625278	39.35736918008568	38.141331754202376	17.40697290508666
105-109	5.302393518581437	24.246609838204364	42.8796069866552	27.571389656559003
110-114	6.245687791519084	29.158740777262704	26.993311343805733	37.60226008741248
115-119	15.663840565865032	25.691881398897916	22.505580986154758	36.1386970490823
120-124	7.762846193902731	48.43119773552454	16.257684960706808	27.54827110986592
125-129	5.092147033382413	44.90992147906754	6.291562485753994	43.70636900179605
130-134	5.7411816884832625	47.35298289059286	6.444184180481341	40.461651240442535
135-139	10.475932233334397	38.94021014787216	18.182699911358746	32.4011577074347
140-144	10.156927153683785	30.421413961220484	15.35065734230515	44.071001542790576
145-149	7.104439410752909	25.200674950663227	32.15069839730345	35.54418724128041
150-154	4.831875639821186	26.300975283722238	43.12737426153724	25.73977481491933
155-159	5.0398262137575225	28.95203062142228	42.49246064602169	23.51568251879851
160-164	6.495184118245047	26.247723474617697	29.58876782939085	37.668324577746404
165-169	7.955133497669181	42.30117472440839	16.15355108137902	33.59014069654341
170-174	6.7339175826148026	39.2473727089172	23.303837473010404	30.714872235457598
175-179	8.32169322570953	47.80214400344058	11.63411016454913	32.24205260630075
180-184	6.7280726555629835	46.09059208591937	10.176649518489	37.00468574002865
185-189	NaN	NaN	NaN	NaN
190-194	NaN	NaN	NaN	NaN
195-199	NaN	NaN	NaN	NaN
200-204	NaN	NaN	NaN	NaN
205-209	NaN	NaN	NaN	NaN
210-214	NaN	NaN	NaN	NaN
215-219	NaN	NaN	NaN	NaN
220-224	NaN	NaN	NaN	NaN
225-229	NaN	NaN	NaN	NaN
230-234	NaN	NaN	NaN	NaN
235-239	NaN	NaN	NaN	NaN
240-244	NaN	NaN	NaN	NaN
245-249	NaN	NaN	NaN	NaN
250-254	NaN	NaN	NaN	NaN
255-259	NaN	NaN	NaN	NaN
260-264	NaN	NaN	NaN	NaN
265-269	NaN	NaN	NaN	NaN
270-274	NaN	NaN	NaN	NaN
275-279	NaN	NaN	NaN	NaN
280-284	NaN	NaN	NaN	NaN
285-289	NaN	NaN	NaN	NaN
290-294	NaN	NaN	NaN	NaN
295-299	NaN	NaN	NaN	NaN
300-301	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	5.25
2	6.0
3	5.0
4	9.0
5	16.0
6	17.75
7	18.5
8	23.0
9	23.75
10	34.75
11	72.5
12	184.5
13	615.25
14	1769.5
15	4554.0
16	10331.75
17	22114.75
18	45299.25
19	88692.75
20	164111.0
21	276327.5
22	419682.0
23	579217.5
24	703611.75
25	725327.0
26	685716.75
27	630895.25
28	560541.75
29	466350.75
30	360244.5
31	271321.75
32	184609.75
33	123699.0
34	83140.75
35	55102.75
36	40132.5
37	31062.5
38	25339.0
39	22577.75
40	19609.0
41	16554.5
42	13733.25
43	10338.5
44	6900.25
45	4573.5
46	3038.5
47	2070.25
48	1385.0
49	934.5
50	644.5
51	468.25
52	333.5
53	255.5
54	206.25
55	200.75
56	192.5
57	192.0
58	189.75
59	203.75
60	447.0
61	429.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	5.995684306436227E-5
2	5.995684306436227E-5
3	5.995684306436227E-5
4	5.236370860254789
5	5.995684306436227E-5
6	23.634687751756285
7	13.148355813485454
8	2.6518561939116223
9	5.995684306436227E-5
10-14	4.199952873921351
15-19	6.095612378210164E-5
20-24	5.995684306436227E-5
25-29	5.995684306436227E-5
30-34	5.995684306436227E-5
35-39	5.995684306436227E-5
40-44	5.995684306436227E-5
45-49	5.995684306436227E-5
50-54	5.995684306436227E-5
55-59	5.995684306436227E-5
60-64	5.995684306436227E-5
65-69	5.995684306436227E-5
70-74	5.995684306436227E-5
75-79	5.995684306436227E-5
80-84	6.095612378210164E-5
85-89	5.995684306436227E-5
90-94	0.5253218733155874
95-99	5.995684306436227E-5
100-104	5.995684306436227E-5
105-109	5.995684306436227E-5
110-114	0.004342873999295308
115-119	5.995684306436227E-5
120-124	6.295468521758038E-5
125-129	6.59525273707985E-5
130-134	7.29474923949741E-5
135-139	6.495324665305913E-5
140-144	6.994965024175599E-5
145-149	7.29474923949741E-5
150-154	7.494605383045284E-5
155-159	7.694461526593159E-5
160-164	6.695180808853787E-5
165-169	8.294029957236781E-5
170-174	8.393958029010718E-5
175-179	0.535000906347611
180-184	22.1206625470986
185-189	100.0
190-194	100.0
195-199	100.0
200-204	100.0
205-209	100.0
210-214	100.0
215-219	100.0
220-224	100.0
225-229	100.0
230-234	100.0
235-239	100.0
240-244	100.0
245-249	100.0
250-254	100.0
255-259	100.0
260-264	100.0
265-269	100.0
270-274	100.0
275-279	100.0
280-284	100.0
285-289	100.0
290-294	100.0
295-299	100.0
300-301	100.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	2.0014396E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.35104530112251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.0353029560722	55.544267380044474
2	3.9352603994101267	4.74994157273028
3	1.5563939246096692	2.8179000075153
4	0.6743016238944186	1.6277923140109012
5	0.4055858461855043	1.2238764888317737
6	0.25959050827918656	0.9399935114939162
7	0.20703338431810928	0.8746276809078843
8	0.14042904901425085	0.6780031918922084
9	0.08055259638560039	0.43752900542313655
>10	0.577875043005851	6.619926392505534
>50	0.07082002798927631	2.9096560018679765
>100	0.05031078802909132	5.359753515648341
>500	0.0030868698655701857	1.2350741840934316
>1k	0.002624459125233327	3.061114770245536
>5k	3.7824972365117776E-4	1.6317959002598457
>10k+	4.5427409217908046E-4	10.288748082529331
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC	405811	2.02759553673266	No Hit
TGAAAACATGCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC	79878	0.39910272585792744	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGTCGTCCTTCTGCGATC	78315	0.39129334704879426	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC	75458	0.37701862199588737	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC	69151	0.3455063045619763	No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC	65022	0.324876154144247	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGGAAAATC	64012	0.31982978651966315	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGACAAATC	62890	0.3142238216931453	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC	56179	0.2806929572094007	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC	53030	0.2649592823085943	No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTCGTCAAATC	49065	0.24514854207941125	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC	48974	0.24469386935283985	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTCAGTAAATC	47866	0.2391578541765637	No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC	37635	0.18803964906060616	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATC	37445	0.18709033237875378	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGTGCGTCCTTCTGCGATC	35973	0.179735626296192	No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTTGTCAAATC	33440	0.16707973600602288	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTCAGCGAATC	32777	0.16376712042671684	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTTTAGTTAGTCTTGCGACGGTC	27883	0.13931472126363442	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTTAATAAATC	25545	0.12763312967326118	No Hit
CGAAAACACCCTTGGCAGATGCCTTCGCGTTCGTCCGTCTTTCGCAAATC	24107	0.12044830131271511	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTACGATC	23952	0.11967385875646709	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC	23178	0.11580664237881573	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC	22364	0.11173956985761647	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGTCGTCCCGCTGCGGTC	21854	0.1091914040273811	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTCCAACGATC	20862	0.1042349716673938	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTTTAGGCAGTCTCACAAGGGTC	20836	0.1041050651740877	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAATAAATC	20781	0.10383026297670936	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTCAGCAAATC	20330	0.10157688495820708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.646655337488076E-4	0.0	0.0
2	4.746583409262013E-4	0.0	0.0
3	4.796547445148982E-4	0.0	0.0
4	4.896475516922918E-4	0.0	0.0
5	4.896475516922918E-4	0.0	0.0
6	5.096331660470793E-4	0.0	0.0
7	5.19625973224473E-4	0.0	0.0
8	5.246223768131698E-4	1.4989210766090568E-5	0.0
9	5.396115875792605E-4	1.4989210766090568E-5	0.0
10-14	5.665921669582234E-4	1.4989210766090568E-5	0.0
15-19	6.145576414097133E-4	1.9985614354787423E-5	0.0
20-24	7.364698889739165E-4	2.2983456508005538E-5	0.0
25-29	8.543850136671624E-4	2.498201794348428E-5	4.996403588696855E-6
30-34	9.673037347717114E-4	2.498201794348428E-5	9.992807177393711E-6
35-39	0.0011281879303277503	2.498201794348428E-5	4.7965474451489814E-5
40-44	0.0013909987590932046	2.9978421532181136E-5	4.996403588696856E-5
45-49	0.0017317534838423303	2.9978421532181136E-5	4.996403588696856E-5
50-54	0.0021124794373010305	2.9978421532181136E-5	4.996403588696856E-5
55-59	0.002665081674210903	2.9978421532181136E-5	4.996403588696856E-5
60-64	0.0033555846501688085	2.9978421532181136E-5	4.996403588696856E-5
65-69	0.004237949523932673	2.9978421532181136E-5	8.99352645965434E-5
70-74	0.005391119472203908	3.197698296765988E-5	8.99352645965434E-5
75-79	0.0067481426868939735	3.9971228709574846E-5	8.99352645965434E-5
80-84	0.008462908398534735	3.9971228709574846E-5	8.99352645965434E-5
85-89	0.010452476307553821	3.9971228709574846E-5	9.892879105619776E-5
90-94	0.012918701118934589	3.9971228709574846E-5	1.3590217761255447E-4
95-99	0.015982495799523506	3.9971228709574846E-5	1.8986333637048054E-4
100-104	0.01984771361573939	3.9971228709574846E-5	1.9485973995917737E-4
105-109	0.024572312849211138	3.9971228709574846E-5	2.0385326641883173E-4
110-114	0.030458076276696035	3.9971228709574846E-5	2.0785038928978922E-4
115-119	0.037399080142113704	3.9971228709574846E-5	2.09848950725268E-4
120-124	0.04566113311638283	3.9971228709574846E-5	2.09848950725268E-4
125-129	0.05484951931599634	3.9971228709574846E-5	2.118475121607467E-4
130-134	0.06499421716248643	3.9971228709574846E-5	2.148453543139648E-4
135-139	0.07595532735536961	3.9971228709574846E-5	2.148453543139648E-4
140-144	0.08800765209202417	3.9971228709574846E-5	2.2383888077361913E-4
145-149	0.10125811440924823	3.9971228709574846E-5	2.3483096866875222E-4
150-154	0.11513512573649487	3.9971228709574846E-5	2.398273722574491E-4
155-159	0.1296666659338608	3.9971228709574846E-5	2.408266529751885E-4
160-164	0.14437407953754888	3.9971228709574846E-5	2.4982017943484284E-4
165-169	0.1590295305439145	3.9971228709574846E-5	2.5481658302353965E-4
170-174	0.17192724676777654	3.9971228709574846E-5	2.578144251767577E-4
175-179	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
180-184	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
185-189	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
190-194	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
195-199	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
200-204	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
205-209	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
210-214	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
215-219	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
220-224	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
225-229	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
230-234	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
235-239	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
240-244	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
245-249	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
250-254	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
255-259	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
260-264	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
265-269	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
270-274	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
275-279	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
280-284	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
285-289	0.17366499593592533	3.9971228709574846E-5	2.598129866122365E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAAAC	537820	0.0	231.5604	1
ATTCTTG	410580	0.0	226.21999	8
ATCCTTG	422430	0.0	224.12828	8
AAGACAT	207080	0.0	223.86247	3
ACATTCT	418310	0.0	221.59567	6
TCCTTGG	474380	0.0	219.2426	9
CGAAGAC	130245	0.0	218.97849	1
GACATTC	169550	0.0	218.01439	5
AACATTC	245015	0.0	217.35765	5
AGACATT	167965	0.0	216.8414	4
AACATCC	343120	0.0	216.71422	5
GAAGACA	217960	0.0	216.26842	2
GAAAACA	559265	0.0	214.07454	2
TTCTTGG	455095	0.0	213.80453	9
CTCCTTG	54555	0.0	213.27232	8
AGACATC	58690	0.0	212.5575	4
AAAACAT	552675	0.0	211.86188	3
TGAAGAC	72965	0.0	211.13847	1
TGTACTG	6165	0.0	210.58417	7
ACATCCT	440220	0.0	210.57782	6
>>END_MODULE