FastQCFastQC Report
Fri 25 Sep 2015
000000000-AJPTB l01n03.3330000003498a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJPTB l01n03.3330000003498a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20014396
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC4058112.02759553673266No Hit
TGAAAACATGCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC798780.39910272585792744No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGTCGTCCTTCTGCGATC783150.39129334704879426No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC754580.37701862199588737No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC691510.3455063045619763No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC650220.324876154144247No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGGAAAATC640120.31982978651966315No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGACAAATC628900.3142238216931453No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC561790.2806929572094007No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC530300.2649592823085943No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTCGTCAAATC490650.24514854207941125No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC489740.24469386935283985No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTCAGTAAATC478660.2391578541765637No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC376350.18803964906060616No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATC374450.18709033237875378No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGTGCGTCCTTCTGCGATC359730.179735626296192No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTTGTCAAATC334400.16707973600602288No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTCAGCGAATC327770.16376712042671684No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTTTAGTTAGTCTTGCGACGGTC278830.13931472126363442No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTTAATAAATC255450.12763312967326118No Hit
CGAAAACACCCTTGGCAGATGCCTTCGCGTTCGTCCGTCTTTCGCAAATC241070.12044830131271511No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTACGATC239520.11967385875646709No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC231780.11580664237881573No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC223640.11173956985761647No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGTCGTCCCGCTGCGGTC218540.1091914040273811No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTCCAACGATC208620.1042349716673938No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTTTAGGCAGTCTCACAAGGGTC208360.1041050651740877No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAATAAATC207810.10383026297670936No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTCAGCAAATC203300.10157688495820708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAC5378200.0231.56041
ATTCTTG4105800.0226.219998
ATCCTTG4224300.0224.128288
AAGACAT2070800.0223.862473
ACATTCT4183100.0221.595676
TCCTTGG4743800.0219.24269
CGAAGAC1302450.0218.978491
GACATTC1695500.0218.014395
AACATTC2450150.0217.357655
AGACATT1679650.0216.84144
AACATCC3431200.0216.714225
GAAGACA2179600.0216.268422
GAAAACA5592650.0214.074542
TTCTTGG4550950.0213.804539
CTCCTTG545550.0213.272328
AGACATC586900.0212.55754
AAAACAT5526750.0211.861883
TGAAGAC729650.0211.138471
TGTACTG61650.0210.584177
ACATCCT4402200.0210.577826
Produced by FastQC (version 0.11.2)