FastQCFastQC Report
Fri 25 Sep 2015
000000000-AJPTB l01n02.3320000003498d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJPTB l01n02.3320000003498d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20014396
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAAAGTGTTC8881634.437620800547766No Hit
TAGCTCGTAACT8690394.342069578317527No Hit
GACTTTCCCTCG8350314.1721518850731245No Hit
AAGAGATGTCGA8296594.145311204994645No Hit
ATGTGCACGACT8169754.081936821875614No Hit
TTAGGGCTCGTA7996113.995179269961482No Hit
TACAGATGGCTC7514443.75451749830472No Hit
ATCCCGAATTTG7408243.7014556921927593No Hit
CTGCTAACGCAA7376783.6857370065027193No Hit
GTTGGTCAATCT7330093.662408798147094No Hit
CGGAGCTATGGT7183193.589011629429137No Hit
TAGGATTGCTCG6966653.480819506119495No Hit
GGATGGTGTTGC6907913.4514706314394896No Hit
ACGTGTACCCAA6800633.3978692137399498No Hit
CAGTGCATATGC6747133.371138454540422No Hit
TCTAGCGTAGTG6623583.309407888202072No Hit
ACGCGCAGATAC6586043.2906513891301037No Hit
TCGAGGACTGCA6446113.2207367137134693No Hit
GCGATATATCGC6367823.1816198700175615No Hit
GGAGACAAGGGA6175373.0854640829530906No Hit
TCACGGGAGTTG6129693.062640511359923No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAC550.0091610096.00000053
TTGGACA1101.8155879E-76.00000052
GGGCTGG550.0091610096.00000054
ATAGGCC550.0091610096.00000051
CACGGGG2050.06.00000052
CGTGCAG4400.06.00000052
CGGTTTA550.0091610096.00000052
GTAGGCC550.0091610096.00000051
CGCGAGT1101.8155879E-76.00000054
GACCCGG550.0091610096.00000054
GCCGCTT550.0091610096.00000055
CGGGGAC550.0091610096.00000052
TGCGCCG550.0091610096.00000052
GCGGACG550.0091610096.00000053
TGGGAGT2050.06.00000054
GCTGTGG1101.8155879E-76.00000055
GTGGTTC550.0091610096.00000056
TTATTCG550.0091610096.00000056
AGGGACC550.0091610096.00000053
TCGCGGT1101.8155879E-76.00000051
Produced by FastQC (version 0.11.2)