##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJPTB l01n01.33100000034980.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20014396
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.13233164768	34.0	34.0	34.0	32.0	34.0
2	33.27169288546104	34.0	34.0	34.0	33.0	34.0
3	33.653692072446255	34.0	34.0	34.0	34.0	34.0
4	33.70278853281408	34.0	34.0	34.0	34.0	34.0
5	33.78168529292615	34.0	34.0	34.0	34.0	34.0
6	37.48335628014955	38.0	38.0	38.0	37.0	38.0
7	37.48037917307122	38.0	38.0	38.0	37.0	38.0
8	37.095364156879874	38.0	38.0	38.0	36.0	38.0
9	36.98719656591186	38.0	38.0	38.0	36.0	38.0
10-14	37.18526603550764	38.0	37.8	38.0	36.2	38.0
15-19	37.250041679998745	38.0	38.0	38.0	36.4	38.0
20-24	37.09955866767101	38.0	37.6	38.0	36.0	38.0
25-29	37.25259348321079	38.0	38.0	38.0	36.6	38.0
30-34	37.151752358652246	38.0	38.0	38.0	36.8	38.0
35-39	37.23865169850742	38.0	38.0	38.0	36.8	38.0
40-44	37.21637623238793	38.0	38.0	38.0	36.4	38.0
45-49	36.907095442700346	38.0	37.6	38.0	35.2	38.0
50-54	36.37720095075565	38.0	37.2	38.0	34.2	38.0
55-59	36.06084873108337	38.0	37.0	38.0	32.8	38.0
60-64	35.04604023024227	38.0	36.2	38.0	26.4	38.0
65-69	34.825977701250636	38.0	36.0	38.0	25.4	38.0
70-74	34.96806004038293	38.0	36.2	38.0	26.0	38.0
75-79	34.6148962177025	38.0	36.0	38.0	24.2	38.0
80-84	34.375324171661234	38.0	35.2	38.0	23.4	38.0
85-89	34.3474972814568	38.0	35.4	38.0	22.8	38.0
90-94	34.130146910254	38.0	35.0	38.0	22.4	38.0
95-99	34.05131581287789	38.0	34.4	38.0	22.0	38.0
100-104	33.98355325836463	38.0	34.2	38.0	21.8	38.0
105-109	34.04996288671414	38.0	34.6	38.0	22.2	38.0
110-114	33.74174366291143	38.0	34.2	38.0	20.6	38.0
115-119	33.54899774142572	38.0	34.0	38.0	19.6	38.0
120-124	33.552142148081806	38.0	34.0	38.0	19.6	38.0
125-129	33.18719437748708	38.0	33.4	38.0	18.6	38.0
130-134	33.09709517089599	37.8	32.8	38.0	18.4	38.0
135-139	33.22797990006793	38.0	33.6	38.0	17.4	38.0
140-144	33.00677844087825	37.4	32.4	38.0	16.6	38.0
145-149	32.91892301921077	37.0	32.0	38.0	16.6	38.0
150-154	32.62854778130702	37.0	31.6	38.0	13.0	38.0
155-159	32.48393751177902	37.0	31.2	38.0	12.0	38.0
160-164	32.52948988318209	37.0	31.2	38.0	12.4	38.0
165-169	32.347693470240124	37.0	30.8	38.0	11.0	38.0
170-174	32.2554714416563	37.0	30.2	38.0	11.0	38.0
175-179	31.93292623969267	37.0	29.4	38.0	11.0	38.0
180-184	31.775543363886676	37.0	28.6	38.0	11.0	38.0
185-189	31.542106711588996	37.0	27.8	38.0	11.0	38.0
190-194	31.17565209562157	36.6	26.2	38.0	11.0	38.0
195-199	31.081922841938376	36.0	26.2	38.0	11.0	38.0
200-204	30.38256571919532	35.8	24.4	38.0	10.8	38.0
205-209	29.98989313492148	35.6	23.4	38.0	10.8	38.0
210-214	30.11199903309598	35.6	24.0	38.0	11.0	38.0
215-219	29.207729356409253	34.0	22.0	38.0	10.4	38.0
220-224	29.124129781383363	34.0	22.0	38.0	10.2	38.0
225-229	28.82667961601239	34.0	21.6	37.8	10.2	38.0
230-234	28.152140319398097	33.8	19.8	37.2	10.0	38.0
235-239	28.087535162190257	33.6	20.2	37.0	10.0	38.0
240-244	28.21898172695294	33.8	20.4	37.2	10.4	38.0
245-249	27.566652423585502	33.2	19.2	37.0	9.8	38.0
250-254	25.980983168315447	28.4	17.0	36.4	9.2	37.4
255-259	25.608966236103253	27.4	16.6	36.4	9.0	37.4
260-264	22.914982405664404	23.4	13.6	32.4	8.2	36.8
265-269	22.219917853129317	22.2	13.0	31.6	8.0	36.8
270-274	21.36959075857198	21.2	11.6	30.2	8.0	36.0
275-279	20.456930501425074	19.8	10.8	29.2	8.0	35.0
280-284	21.034377015424297	20.8	10.0	29.6	8.0	35.8
285-289	21.609318922239773	21.8	9.8	31.6	8.2	36.2
290-294	20.44031132390905	20.0	9.4	28.8	8.0	35.0
295-299	20.008706652951204	19.4	9.0	28.4	7.8	34.6
300-301	15.588453456202227	14.5	8.0	21.0	7.0	28.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-6.583608603412419
1101	2	-1.3100579245668982
1101	3	-0.21766522607634187
1101	4	-0.06274710881447021
1101	5	-0.0283231821525618
1101	6	-0.09189873267980886
1101	7	0.021184519715511385
1101	8	0.16807586092504323
1101	9	0.18324143713986274
1101	10-14	0.15805443637667338
1101	15-19	0.12172496629800378
1101	20-24	0.23336131993016096
1101	25-29	0.1399967644783615
1101	30-34	0.18818684286369347
1101	35-39	0.15490669891241993
1101	40-44	0.13955622777490362
1101	45-49	0.22989227659721934
1101	50-54	0.3253210633488166
1101	55-59	0.4004755831697224
1101	60-64	0.48951613941245853
1101	65-69	0.6923942205490263
1101	70-74	0.584532386221781
1101	75-79	0.5105910289618336
1101	80-84	0.6409937708588274
1101	85-89	0.6637993229327961
1101	90-94	0.6830727322405252
1101	95-99	0.750123462843554
1101	100-104	0.6928429882408125
1101	105-109	0.6425485607851797
1101	110-114	0.6056612737467901
1101	115-119	0.6644774536065299
1101	120-124	0.6339698897902935
1101	125-129	0.7000219174649018
1101	130-134	0.7747019174451211
1101	135-139	0.7615386779901314
1101	140-144	0.7816200306238699
1101	145-149	0.8461937148830145
1101	150-154	0.8218504278267815
1101	155-159	0.8175565094648718
1101	160-164	0.8997747679272479
1101	165-169	0.8068668992241967
1101	170-174	0.8883872800391828
1101	175-179	0.976779365320219
1101	180-184	0.8627829164681451
1101	185-189	0.7520191363588111
1101	190-194	0.8011389548402015
1101	195-199	0.7964086610224008
1101	200-204	0.949332483869437
1101	205-209	0.9516163392989512
1101	210-214	0.8140758251876505
1101	215-219	0.4541779326738542
1101	220-224	0.24394348679971145
1101	225-229	0.47602393134168963
1101	230-234	0.09841688870601573
1101	235-239	0.4311523841367837
1101	240-244	0.5237191276451831
1101	245-249	0.19312497865941936
1101	250-254	-0.6858390683308428
1101	255-259	-0.334858387160228
1101	260-264	-0.3541451601776586
1101	265-269	0.142501462978327
1101	270-274	0.1922184742324191
1101	275-279	0.11669015136738636
1101	280-284	-0.9957646905017086
1101	285-289	-0.1867394552243482
1101	290-294	-1.04526870781811
1101	295-299	-0.7276783454747715
1101	300-301	0.04695578965506364
1102	1	0.045927805497292695
1102	2	-0.13534437627073004
1102	3	-0.07832671504557709
1102	4	-0.01459732410657466
1102	5	-0.048480259774493106
1102	6	-0.08267001595935852
1102	7	0.08698184476151738
1102	8	0.1357896425611287
1102	9	0.16604540668897272
1102	10-14	0.1486011389149553
1102	15-19	0.19730893775216884
1102	20-24	0.2557423632042912
1102	25-29	0.2004569949236128
1102	30-34	0.2035466486225772
1102	35-39	0.14283148088593833
1102	40-44	0.10309741164756048
1102	45-49	0.19050300158072275
1102	50-54	0.3523932590157841
1102	55-59	0.4487126216609667
1102	60-64	0.6217012137360669
1102	65-69	0.6693187195475474
1102	70-74	0.5117743044824721
1102	75-79	0.5904803989149983
1102	80-84	0.7306511532755167
1102	85-89	0.6849279728309057
1102	90-94	0.7167822876180523
1102	95-99	0.6849735979207594
1102	100-104	0.6209154355545152
1102	105-109	0.5853836754304353
1102	110-114	0.6275490249440452
1102	115-119	0.6493186725688034
1102	120-124	0.6557706855474947
1102	125-129	0.7855063599874299
1102	130-134	0.7866650646636586
1102	135-139	0.761057165395151
1102	140-144	0.8346901809555405
1102	145-149	0.7422250269542516
1102	150-154	0.8088566225262284
1102	155-159	0.8762496340863919
1102	160-164	0.8824802666036575
1102	165-169	0.9826255949744436
1102	170-174	0.92018500918212
1102	175-179	1.016696728115889
1102	180-184	0.8995472074185678
1102	185-189	0.9135691732453779
1102	190-194	0.9609442290480352
1102	195-199	0.9893102515043637
1102	200-204	1.1604138778028315
1102	205-209	1.2803115333292823
1102	210-214	1.1422488144828549
1102	215-219	1.1227156964425546
1102	220-224	1.2327535017242859
1102	225-229	1.291657385818855
1102	230-234	1.125034312138002
1102	235-239	1.2321021370981313
1102	240-244	1.2392908312440625
1102	245-249	1.0594483734358633
1102	250-254	1.1346147419347048
1102	255-259	0.8361153005935549
1102	260-264	0.9271905473343516
1102	265-269	1.0796340997053058
1102	270-274	1.1237479760564675
1102	275-279	0.8716422583543988
1102	280-284	0.8859613874978578
1102	285-289	0.35152994911988245
1102	290-294	-0.16911305204994065
1102	295-299	0.16720647294396684
1102	300-301	0.8785867751479977
1103	1	0.21659573355570672
1103	2	-0.23694560745951776
1103	3	-0.06905664326369276
1103	4	-0.0023745186698747034
1103	5	0.0071745620472469795
1103	6	0.11758359383332362
1103	7	-0.17752415068705574
1103	8	-0.18825057682198576
1103	9	-0.2924966681982184
1103	10-14	0.08731434972123964
1103	15-19	0.19756663091768445
1103	20-24	0.22890591799673388
1103	25-29	0.02714202096917262
1103	30-34	0.022943044961245107
1103	35-39	0.18236854870930586
1103	40-44	0.20029408473457266
1103	45-49	0.2210200528830839
1103	50-54	0.3521001814235021
1103	55-59	0.5477311302094208
1103	60-64	0.6429664452234434
1103	65-69	0.6804577525424946
1103	70-74	0.591357856512083
1103	75-79	0.5880729384270253
1103	80-84	0.723564483121379
1103	85-89	0.6831921502783658
1103	90-94	0.7373026965342788
1103	95-99	0.4665180199037309
1103	100-104	0.6889499224967537
1103	105-109	0.3957254553030367
1103	110-114	0.6543929923082459
1103	115-119	0.6951151766435544
1103	120-124	0.7713328550760608
1103	125-129	0.7944499340274902
1103	130-134	0.19848881563010679
1103	135-139	0.7927375799365279
1103	140-144	0.9051346125046251
1103	145-149	0.8903549197481979
1103	150-154	0.9770524362787327
1103	155-159	0.39066455473631834
1103	160-164	0.2276629305771607
1103	165-169	0.746697503598952
1103	170-174	0.8786144393163937
1103	175-179	1.0043633605854367
1103	180-184	1.025666348300394
1103	185-189	1.157735306828613
1103	190-194	1.214934873402111
1103	195-199	1.1944254650068231
1103	200-204	1.4249904221690848
1103	205-209	1.4181266538732231
1103	210-214	1.3724363542054725
1103	215-219	1.3192265130279672
1103	220-224	1.355908161114705
1103	225-229	1.3532710820428342
1103	230-234	1.2704752369690198
1103	235-239	1.428742863106578
1103	240-244	1.6484327887943877
1103	245-249	1.4733383944691205
1103	250-254	1.8141554306218062
1103	255-259	1.2304637244715586
1103	260-264	1.638175191887239
1103	265-269	0.995524800248095
1103	270-274	1.0808156656605377
1103	275-279	1.3167704105076936
1103	280-284	1.0222466177152754
1103	285-289	1.107039756770515
1103	290-294	0.43201336892009223
1103	295-299	0.16181418112007506
1103	300-301	1.0960250255988164
2116	1	0.15757672958010005
2116	2	-0.21765240511421524
2116	3	-0.12344000740080219
2116	4	0.014447824342859406
2116	5	-0.02469159932766729
2116	6	0.07692939101843876
2116	7	-0.050934370800433726
2116	8	-0.23231075090370723
2116	9	0.14240639526354215
2116	10-14	0.1805912278663584
2116	15-19	0.16650985459587986
2116	20-24	0.2765051813979724
2116	25-29	0.06285422719602707
2116	30-34	0.2099180940843013
2116	35-39	0.1849739567475197
2116	40-44	0.1721959878397996
2116	45-49	0.19569394644834404
2116	50-54	0.3890625221829964
2116	55-59	0.5154447526425159
2116	60-64	0.6306930022139738
2116	65-69	0.6319248490445375
2116	70-74	0.6059409641639064
2116	75-79	0.6136338206220913
2116	80-84	0.7492023385646291
2116	85-89	0.6764610063392595
2116	90-94	0.7157288204234007
2116	95-99	0.5373921806762851
2116	100-104	0.7008322841909873
2116	105-109	0.7462484200098558
2116	110-114	0.7445330024395886
2116	115-119	0.5943011365646456
2116	120-124	0.6547208991045395
2116	125-129	0.8534711526235057
2116	130-134	0.5173001608822361
2116	135-139	0.7462535645611013
2116	140-144	0.9116535902871377
2116	145-149	0.729243626697972
2116	150-154	0.9360882523253053
2116	155-159	0.41211564486627594
2116	160-164	0.09338224730699807
2116	165-169	0.9185335344525996
2116	170-174	1.104247685591858
2116	175-179	0.7552178504930609
2116	180-184	0.9926685313722565
2116	185-189	1.0916161649468847
2116	190-194	1.1548407234972053
2116	195-199	1.1313314732945088
2116	200-204	1.2542402891947404
2116	205-209	1.44848952012984
2116	210-214	1.0942866103880178
2116	215-219	1.4336665539224427
2116	220-224	1.6310565898267697
2116	225-229	1.2175579281325284
2116	230-234	1.3678401177620216
2116	235-239	1.444973957234243
2116	240-244	1.5653858094544155
2116	245-249	1.5662109951464487
2116	250-254	1.2501850012723068
2116	255-259	0.9601198570702252
2116	260-264	1.3018338457941532
2116	265-269	1.5539833967466272
2116	270-274	1.2632335279172437
2116	275-279	1.0798127420072632
2116	280-284	1.803509811439831
2116	285-289	1.2442437344659645
2116	290-294	0.8018974975379223
2116	295-299	0.8012110146445792
2116	300-301	0.9730723117580631
1117	1	0.28130521171534895
1117	2	0.06194223918200947
1117	3	0.020976395072786147
1117	4	0.05278187496759301
1117	5	-0.015460442241760575
1117	6	0.09461745341758387
1117	7	0.08833068421529333
1117	8	0.2434350846242026
1117	9	0.2748473808993097
1117	10-14	0.22891620668597312
1117	15-19	0.15937981572525928
1117	20-24	0.13766036118862957
1117	25-29	-0.06020783135749497
1117	30-34	0.18232262729996762
1117	35-39	0.1563028052302613
1117	40-44	0.18703288860448453
1117	45-49	0.2516564390208984
1117	50-54	0.3895851513748525
1117	55-59	0.43894055891262695
1117	60-64	0.5501256345186576
1117	65-69	0.6960448840465077
1117	70-74	0.5457238464038596
1117	75-79	0.6643452469904219
1117	80-84	0.6800521477569035
1117	85-89	0.6356041766482647
1117	90-94	0.6459014049093312
1117	95-99	0.4406563989165946
1117	100-104	0.6130145544324392
1117	105-109	0.7235722959962558
1117	110-114	0.6949375797525335
1117	115-119	0.6016199523972929
1117	120-124	0.7481749802768007
1117	125-129	0.8584007487324996
1117	130-134	0.4982792005113126
1117	135-139	0.6873600687690598
1117	140-144	0.8575981222392883
1117	145-149	0.8525234249199798
1117	150-154	0.9097153603425809
1117	155-159	0.21331346606844193
1117	160-164	0.9049521453172744
1117	165-169	0.7475127163188588
1117	170-174	0.8747975959595422
1117	175-179	1.0138984781916491
1117	180-184	0.9668883452413617
1117	185-189	1.0529754049896525
1117	190-194	1.0558305264785268
1117	195-199	1.053613088862896
1117	200-204	1.2194541959156595
1117	205-209	1.2909532098514802
1117	210-214	1.2155966900205115
1117	215-219	1.5265302303520407
1117	220-224	1.5886569883504507
1117	225-229	1.1467198578995017
1117	230-234	1.309669194197415
1117	235-239	1.4346059437132617
1117	240-244	1.5889750848288529
1117	245-249	1.4649862366755073
1117	250-254	1.1340430043446048
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2108	250-254	-1.817015486629618
2108	255-259	-0.9909142227577377
2108	260-264	-0.6018422754140076
2108	265-269	-1.2083959984070312
2108	270-274	-1.5241570009314138
2108	275-279	-1.6832783401350433
2108	280-284	-0.3293323541333315
2108	285-289	-0.3821924125496672
2108	290-294	-0.48138041552731536
2108	295-299	0.41138051096786654
2108	300-301	-0.4534686962018917
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	11.0
6	0.0
7	0.0
8	0.0
9	0.0
10	30.0
11	476.0
12	2069.0
13	3103.0
14	5863.0
15	13126.0
16	21005.0
17	35521.0
18	97930.0
19	301657.0
20	538506.0
21	630530.0
22	521021.0
23	462853.0
24	469681.0
25	514254.0
26	568789.0
27	646202.0
28	740554.0
29	849444.0
30	986920.0
31	1171720.0
32	1447803.0
33	1847028.0
34	2395424.0
35	2957711.0
36	2473057.0
37	312107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	4.581297382144332	86.05476777815329	4.295088395373011	5.068846444329371
2	82.48406796787671	9.205798666120128	4.247207859782528	4.062925506220623
3	3.912134045913751	6.028930375915416	4.334979681625166	85.72395589654566
4	3.8765196811335203	5.080563010744866	85.23091578681665	5.812001521304964
5	4.085684124567137	5.841110568612713	4.330627814099412	85.74257749272074
6	5.1489437902597714	5.281863114929874	29.997108081602864	59.5720850132075
7	5.93072106697599	59.915557781508866	6.598375489322786	27.555345662192355
8	9.296843132313361	66.11768349142288	15.473846924983398	9.111626451280369
9	8.222531421882529	22.932852932459216	63.41282045183877	5.431795193819489
10-14	37.2005570390433	22.43524211272726	24.004145815841756	16.360055032387688
15-19	5.317995107121893	39.427324212032175	42.90500098029438	12.349679700551542
20-24	20.101956611630946	42.86895892336696	29.37725725023128	7.651827214770808
25-29	36.99412263053055	5.019332084765385	48.39626336962654	9.590281915077528
30-34	27.158620940509117	13.941544270588457	39.44834102896146	19.45149375994097
35-39	4.776598192919833	85.37541846150064	4.87771445998893	4.9702688855905945
40-44	36.8386776230535	5.314345922412601	21.57100513310827	36.27597132142563
45-49	20.636756838114458	21.337534831573784	52.382104266790286	5.643604063521475
50-54	31.32818576879508	30.213777251400792	27.187632654594818	11.27040432520931
55-59	36.00254596893914	11.691588409615804	34.45927389021816	17.8465917312269
60-64	28.739938236420365	20.09291017899926	28.652247303739152	22.51490428084122
65-69	34.125415001530904	17.34581189208721	27.797775839616147	20.73099726676574
70-74	31.346350704573272	11.689191133736616	32.63883315119766	24.325625010492455
75-79	19.29264373062893	13.16158818577679	26.878574928911124	40.667193154683154
80-84	31.446530655152817	17.38430720625726	30.497394274038115	20.671767864551814
85-89	32.21262967673649	20.879888184417766	27.266962600497724	19.64051953834802
90-94	25.902608843719598	21.575690763203102	28.784299331920483	23.737401061156817
95-99	22.93570387113292	19.36391666477386	26.854717355824864	30.845662108268357
100-104	25.64486946632851	18.75257933246988	27.707872774978963	27.894678426222647
105-109	19.935717431380525	17.018960134061185	36.281063022285004	26.76425941227329
110-114	21.900349268805876	19.953706294433044	30.435797574384505	27.71014686237658
115-119	27.5767813304028	15.336581237505612	34.11986567500385	22.966771757087738
120-124	25.021090332254847	17.91406756089156	29.27723140072123	27.787610706132366
125-129	24.16814399588248	20.54911569246745	34.488862644651846	20.793877666998224
130-134	22.21793497206484	22.979857035812696	27.887482716804215	26.914725275318258
135-139	27.476157071165414	21.085614701899424	28.67358796268839	22.764640264246772
140-144	29.71868051372175	17.488102256381303	33.846994595366795	18.94622263453015
145-149	33.19560804238847	22.09020327212092	26.446361627962485	18.26782705752812
150-154	26.760387534908148	26.445514972362776	23.824673724138023	22.969423768591053
155-159	20.239649667923796	26.35035945651482	31.809995432284644	21.59999544327674
160-164	17.496813691338282	27.176217038803056	38.66627930522603	16.66068996463263
165-169	14.471138225656402	39.616922886075706	31.666654242268216	14.245284645999675
170-174	16.773320808206442	44.39386070658172	25.240392391043372	13.592426094168466
175-179	22.181216043918496	27.969477589276	33.6259648961239	16.223341470681603
180-184	25.853121985855093	35.87040915396102	20.999625329440587	17.276843530743303
185-189	24.939982409249684	31.621039993929966	23.367235387360598	20.071742209459757
190-194	27.34744681015201	23.4061302473713	30.641779657741342	18.60464328473535
195-199	23.19774926652495	26.855768748135528	27.98582411402137	21.960657871318155
200-204	19.04351138227035	25.95597883429962	27.99379250362442	27.00671727980561
205-209	17.9814116677991	32.24188624750511	35.33284838208603	14.443853702609768
210-214	25.46305050186209	31.820817377391226	30.225078936733436	12.491053184013248
215-219	20.22216104177325	39.39311501706008	28.01682681966571	12.367897121500958
220-224	21.936450401693623	42.42305046325935	26.242739533864224	9.397759601182809
225-229	25.483993460302695	35.046059378700775	25.09248299547795	14.377464165518575
230-234	26.58217589656047	29.437667312798656	30.19830528478834	13.781851505852535
235-239	17.422941117665818	21.000814054564522	42.809023462686575	18.76722136508309
240-244	23.420873878550243	16.86960122627812	45.29625719663704	14.413267698534598
245-249	25.140657838882273	18.611565562047776	45.282415886494434	10.965360712575515
250-254	26.15970998296865	21.78678681439456	39.67995476373212	12.373548438904677
255-259	25.654375399387774	21.40245820815502	39.61200662931246	13.331159763144736
260-264	24.389916235638303	33.27872193990414	30.13189494906633	12.19946687539123
265-269	23.00076054304026	30.45372959128983	31.63855849788508	14.90695136778483
270-274	27.336000495163887	37.41945167874168	24.568024168378585	10.676523657715844
275-279	26.476381304676185	34.580549435290095	26.213858090370053	12.729211169663671
280-284	34.32496251670686	28.100309100704045	21.638497241938516	15.936231140650579
285-289	37.58040505170707	24.134701529395137	22.568508856855594	15.716384562042208
290-294	34.37897932564858	23.092047661163047	24.83400587965147	17.694967133536903
295-299	26.885773020901098	24.697957475962863	31.394655277870708	17.02161422526533
300-301	27.384083551711697	20.54828718556332	36.9245173102634	15.143111952461588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.25
4	0.5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.75
10	1.25
11	4.75
12	6.0
13	8.5
14	19.25
15	33.25
16	64.75
17	102.25
18	150.0
19	240.5
20	387.0
21	531.75
22	748.5
23	1011.5
24	1205.75
25	1613.5
26	1812.5
27	2292.5
28	3549.25
29	6293.75
30	8549.0
31	8275.75
32	12900.0
33	23205.5
34	42076.25
35	85666.0
36	95275.75
37	150163.0
38	381254.0
39	705585.25
40	458791.25
41	513254.75
42	541857.5
43	484479.5
44	426577.0
45	376487.0
46	315707.5
47	418001.75
48	411400.0
49	438027.5
50	200124.75
51	102293.75
52	76054.0
53	62799.5
54	47232.0
55	64267.5
56	38619.75
57	19867.0
58	14744.5
59	12563.25
60	10150.0
61	8188.5
62	8322.25
63	15397.5
64	24319.0
65	15052.75
66	7123.0
67	3686.5
68	1973.5
69	1599.0
70	1125.25
71	592.0
72	337.75
73	285.25
74	292.25
75	317.25
76	203.5
77	79.5
78	42.75
79	32.0
80	27.25
81	22.0
82	22.25
83	19.0
84	21.5
85	18.25
86	15.75
87	11.0
88	10.5
89	8.25
90	11.75
91	9.75
92	6.25
93	5.5
94	8.25
95	10.25
96	8.25
97	21.0
98	20.0
99	11.5
100	66.6666666666668
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	9.992807177393713E-6
35-39	4.996403588696856E-5
40-44	5.995684306436227E-5
45-49	5.89575623466229E-5
50-54	5.995684306436227E-5
55-59	5.995684306436227E-5
60-64	5.995684306436227E-5
65-69	5.995684306436227E-5
70-74	5.995684306436227E-5
75-79	5.995684306436227E-5
80-84	5.995684306436227E-5
85-89	5.995684306436227E-5
90-94	5.995684306436227E-5
95-99	6.095612378210164E-5
100-104	6.095612378210164E-5
105-109	6.095612378210164E-5
110-114	5.995684306436227E-5
115-119	6.195540449984101E-5
120-124	6.195540449984101E-5
125-129	6.295468521758038E-5
130-134	6.495324665305913E-5
135-139	6.695180808853787E-5
140-144	6.395396593531976E-5
145-149	6.695180808853787E-5
150-154	6.59525273707985E-5
155-159	6.994965024175599E-5
160-164	6.795108880627725E-5
165-169	6.59525273707985E-5
170-174	7.29474923949741E-5
175-179	6.795108880627725E-5
180-184	6.59525273707985E-5
185-189	6.89503695240166E-5
190-194	6.395396593531976E-5
195-199	6.89503695240166E-5
200-204	7.29474923949741E-5
205-209	6.495324665305913E-5
210-214	6.59525273707985E-5
215-219	6.89503695240166E-5
220-224	6.495324665305913E-5
225-229	6.994965024175599E-5
230-234	6.295468521758038E-5
235-239	6.395396593531976E-5
240-244	6.495324665305913E-5
245-249	5.995684306436227E-5
250-254	6.395396593531976E-5
255-259	6.395396593531976E-5
260-264	6.59525273707985E-5
265-269	6.295468521758038E-5
270-274	6.195540449984101E-5
275-279	6.795108880627725E-5
280-284	6.295468521758038E-5
285-289	6.495324665305913E-5
290-294	6.195540449984101E-5
295-299	6.095612378210164E-5
300-301	6.24550448587107E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	2.0014396E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.773320833162311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22868448462239	3.5431732606868
2	11.655284800665697	1.112688275109152
3	4.285373042724878	0.6136638126813246
4	2.6796528061365508	0.5116337026069377
5	1.6074448032143092	0.3836424883670676
6	0.914058190925789	0.26178558032812327
7	0.5143624204620602	0.17186518001711404
8	0.5632472954409021	0.2150848039640308
9	0.4160411617310634	0.17873081502695418
>10	2.0384075569511455	1.7261697282717603
>50	0.20480568422179066	0.6917193977392883
>100	0.8107447777261708	8.112995524422006
>500	0.030008523143254497	0.9915660349560474
>1k	0.03542296770325072	3.6146528483985447
>5k	0.006647912191612725	2.194793403213606
>10k+	0.009813572139142406	75.67583514421133
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	3048855	15.233310063416353	No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG	1999356	9.98958949348259	No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	1370337	6.846756704524084	No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG	651677	3.256041301471201	No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	649262	3.243974986804498	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG	566847	2.832196385042047	No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	482742	2.411973861214698	No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	431198	2.154439234638907	No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG	428784	2.142377916375793	No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	334755	1.672571083334216	No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	314813	1.5729328029684235	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	275688	1.3774485125606588	No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	215996	1.079203189544166	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	203777	1.0181521340938793	No Hit
AGCTCCGCAAGTGTATTCCTATACTGTTGCGGTTAAAAAGCTCGTAGTTG	201711	1.0078295642796316	No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT	199790	0.9982314729857448	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	185097	0.924819315057022	No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG	181606	0.9073768701288812	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	180402	0.9013612002080902	No Hit
AACCCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG	168949	0.8441373899067451	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	147010	0.7345212915743248	No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	142252	0.7107484032993052	No Hit
AGCTCCAATAGCGTACGTTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	142111	0.710043910393299	No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG	122383	0.6114748603954874	No Hit
AGCTCCAATAGCGTATGTTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	108980	0.5445080630961834	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG	99506	0.49717213549686934	No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	95335	0.47633213612841474	No Hit
AGCTCTCAAGGTGTATATCGCCATTGCTGCGGTTAAAAAGCTCGTAGTTG	90070	0.45002607123392585	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG	87210	0.43573635697025276	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGCTCGTAGTTG	87159	0.4354815403872293	No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	85775	0.4285665178204728	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGAGGTTAAAAAGCTCGTAGTTG	79862	0.3990227834005083	No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG	69043	0.344966692974397	No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	66049	0.33000746062983866	No Hit
CAACAGACCGCCCAACCCCGTCAGCGTCGCCGCTTCGATCAAGAACGAAA	64509	0.32231299910324546	No Hit
AGCTCCAATAGCGTAACTTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG	63946	0.3195000238828091	No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG	61111	0.3053352197088536	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	52612	0.26287078560851895	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGTTCGTAGTTG	51725	0.25843897562534485	No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	50740	0.2535175180904785	No Hit
AGCTCCAATAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	48168	0.24066676806035014	No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	47431	0.23698441861548059	No Hit
AGCTCTCAAAATGCATAGAACCATTGCTGCGGTTAAAAAGCTCGTAGTTG	45284	0.22625714011054845	No Hit
AGCTCCACTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT	43893	0.21930714271867108	No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	43129	0.21548989037690672	No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	41033	0.2050174284549981	No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	38664	0.19318094835337524	No Hit
CAACAGACCGCCCAACCCCGTCAGCGTCGCCGCCTCGATCAAGAACGAAA	33879	0.1692731571814608	No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	33669	0.16822391242783444	No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG	32213	0.16094914880269182	No Hit
AGCTCTTTTAGTGTATATTAAATTTGTTGCAGTTAAAAAGCTCGTAGTTG	29158	0.1456851358392229	No Hit
GGCTCTAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCT	27275	0.13627690788170674	No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	27165	0.13572730348695008	No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	26054	0.13017629909990788	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTTG	26020	0.1300064213778922	No Hit
AGCTCCAATAGTGTATATTAACGTTGCTGCAGTTAAAAAGCTCGTAGTTG	26014	0.12997644295636002	No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	25944	0.12962669470515123	No Hit
CCAGGGGCCAATCAAGAACGAAAGTGCCTGACTGACTTACAGATGGCTCA	25487	0.12734333826511676	No Hit
CAGTCAATCAAGAACGAAAGTGCCTGACTGACTGTTGGTCAATCTATCTC	25012	0.12497004656048577	No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	24376	0.12179233387807455	No Hit
AGCTCTCATAGCGTATATCATCATTGCTGCGGTTAAAAAGCTCGTAGTTG	23523	0.11753040161691614	No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG	23413	0.11698079722215948	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCT	22339	0.11161465976789907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	7.944281706028001E-4	0.0	0.0
2	7.994245741914969E-4	0.0	0.0
3	8.094173813688907E-4	0.0	0.0
4	8.194101885462844E-4	0.0	0.0
5	8.194101885462844E-4	0.0	0.0
6	8.194101885462844E-4	0.0	0.0
7	8.244065921349812E-4	0.0	0.0
8	8.294029957236781E-4	0.0	0.0
9	8.294029957236781E-4	0.0	0.0
10-14	8.443922064897688E-4	0.0	0.0
15-19	8.733713473042104E-4	0.0	0.0
20-24	9.50315962570142E-4	0.0	0.0
25-29	0.0010272605778360736	0.0	0.0
30-34	0.001225118159948469	0.0	0.0
35-39	0.001404988689141556	0.0	0.0
40-44	0.001671796640777968	0.0	0.0
45-49	0.0020145499269625727	0.0	0.0
50-54	0.0023892801961148367	0.0	0.0
55-59	0.002947878117331145	0.0	0.0
60-64	0.003616396917498784	0.0	0.0
65-69	0.004510753159875522	0.0	0.0
70-74	0.005652931020251622	0.0	4.996403588696856E-6
75-79	0.007016949199965865	0.0	4.996403588696856E-6
80-84	0.008797667438977424	0.0	4.996403588696856E-6
85-89	0.010941124578528375	0.0	4.996403588696856E-6
90-94	0.013533258760344304	0.0	1.0992087895133082E-5
95-99	0.016699979354860374	0.0	1.4989210766090568E-5
100-104	0.02064513962849541	0.0	1.4989210766090568E-5
105-109	0.025548610110442505	0.0	1.9985614354787423E-5
110-114	0.03159725629491892	1.9985614354787424E-6	1.9985614354787423E-5
115-119	0.038684155145126534	4.996403588696856E-6	1.9985614354787423E-5
120-124	0.0473179405463947	4.996403588696856E-6	2.797986009670239E-5
125-129	0.05696599587616833	4.996403588696856E-6	2.9978421532181136E-5
130-134	0.06763431681875384	4.996403588696856E-6	3.197698296765988E-5
135-139	0.07901812275524078	4.996403588696856E-6	3.9971228709574846E-5
140-144	0.09166901664182123	4.996403588696856E-6	4.097050942731421E-5
145-149	0.1051762941035043	7.99424574191497E-6	4.8964755169229186E-5
150-154	0.11912225580027495	9.493166818524027E-5	4.996403588696856E-5
155-159	0.13394558596722078	9.493166818524027E-5	5.096331660470793E-5
160-164	0.14934350254686676	9.493166818524027E-5	6.695180808853787E-5
165-169	0.1650571918333184	9.493166818524027E-5	7.494605383045284E-5
170-174	0.18078786889197157	9.892879105619776E-5	7.494605383045284E-5
175-179	0.19706515250322817	9.992807177393712E-5	7.494605383045284E-5
180-184	0.21334543395663802	9.992807177393712E-5	7.494605383045284E-5
185-189	0.22998345790699853	9.992807177393712E-5	7.594533454819221E-5
190-194	0.2469112732655035	9.992807177393712E-5	8.493886100784655E-5
195-199	0.2638051130796053	1.0392519464489461E-4	1.0292591392715525E-4
200-204	0.28142143285263266	1.0592375608037335E-4	1.1391800182228831E-4
205-209	0.2989318288695796	1.2291152828194265E-4	1.2990649330611828E-4
210-214	0.31669104578524376	1.9885686283013484E-4	1.3490289689481512E-4
215-219	0.3344632533502385	2.428252144106672E-4	1.3989930048351196E-4
220-224	0.3521415285277657	2.6980579378963024E-4	1.4489570407220882E-4
225-229	0.3698188044245752	2.6980579378963024E-4	1.4489570407220882E-4
230-234	0.3874311270747316	2.7180435522510893E-4	1.5089138837864506E-4
235-239	0.40481861156339666	2.748021973783271E-4	1.598849148382994E-4
240-244	0.4220771888394733	2.748021973783271E-4	1.8486693278178365E-4
245-249	0.43897802361859933	2.748021973783271E-4	1.9985614354787424E-4
250-254	0.4563005548606113	2.748021973783271E-4	2.088496700075286E-4
255-259	0.4733153076415596	2.748021973783271E-4	2.1284679287848608E-4
260-264	0.4897824545891867	2.748021973783271E-4	2.318331265155341E-4
265-269	0.5068062008965946	2.758014780960665E-4	2.3882809153970972E-4
270-274	0.5231854111410607	2.797986009670239E-4	2.4682233728162467E-4
275-279	0.5402191502556459	2.847950045557208E-4	2.498201794348428E-4
280-284	0.5574607397595212	2.847950045557208E-4	2.498201794348428E-4
285-289	0.5752339466052335	2.867935659911995E-4	2.5481658302353965E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTA	778210	0.0	293.83997	9
ATAGCGT	669415	0.0	293.39365	8
CTCCAAT	790455	0.0	293.1862	3
AGCTCCA	994300	0.0	293.1382	1
AATAGCG	665385	0.0	292.7832	7
CCAATAG	721195	0.0	292.36984	5
AGCTCTC	477845	0.0	292.2682	1
GCTCCAA	928740	0.0	292.0078	2
TCCAATA	793110	0.0	291.89227	4
GCTCTCA	249390	0.0	288.99088	2
CTCATGG	147450	0.0	285.09662	5
TCTCATG	147875	0.0	284.50665	4
CAATAGC	688280	0.0	282.58542	6
CTCTCTA	218800	0.0	281.9218	3
CTAAGTG	218990	0.0	280.88916	7
CTCTAAG	219125	0.0	280.844	5
CTCTCAT	204650	0.0	279.37424	3
CTCCAGT	101890	0.0	278.72852	3
TAAGTGT	221305	0.0	278.2508	8
TAGTGTA	84365	0.0	276.83456	9
>>END_MODULE