FastQCFastQC Report
Fri 25 Sep 2015
000000000-AJPTB l01n01.33100000034980.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJPTB l01n01.33100000034980.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20014396
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG304885515.233310063416353No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG19993569.98958949348259No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG13703376.846756704524084No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG6516773.256041301471201No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG6492623.243974986804498No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG5668472.832196385042047No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG4827422.411973861214698No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG4311982.154439234638907No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG4287842.142377916375793No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG3347551.672571083334216No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG3148131.5729328029684235No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG2756881.3774485125606588No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG2159961.079203189544166No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG2037771.0181521340938793No Hit
AGCTCCGCAAGTGTATTCCTATACTGTTGCGGTTAAAAAGCTCGTAGTTG2017111.0078295642796316No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT1997900.9982314729857448No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG1850970.924819315057022No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG1816060.9073768701288812No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG1804020.9013612002080902No Hit
AACCCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG1689490.8441373899067451No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1470100.7345212915743248No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1422520.7107484032993052No Hit
AGCTCCAATAGCGTACGTTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1421110.710043910393299No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG1223830.6114748603954874No Hit
AGCTCCAATAGCGTATGTTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1089800.5445080630961834No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG995060.49717213549686934No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG953350.47633213612841474No Hit
AGCTCTCAAGGTGTATATCGCCATTGCTGCGGTTAAAAAGCTCGTAGTTG900700.45002607123392585No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG872100.43573635697025276No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGCTCGTAGTTG871590.4354815403872293No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG857750.4285665178204728No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGAGGTTAAAAAGCTCGTAGTTG798620.3990227834005083No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG690430.344966692974397No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG660490.33000746062983866No Hit
CAACAGACCGCCCAACCCCGTCAGCGTCGCCGCTTCGATCAAGAACGAAA645090.32231299910324546No Hit
AGCTCCAATAGCGTAACTTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG639460.3195000238828091No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG611110.3053352197088536No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG526120.26287078560851895No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGTTCGTAGTTG517250.25843897562534485No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG507400.2535175180904785No Hit
AGCTCCAATAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG481680.24066676806035014No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG474310.23698441861548059No Hit
AGCTCTCAAAATGCATAGAACCATTGCTGCGGTTAAAAAGCTCGTAGTTG452840.22625714011054845No Hit
AGCTCCACTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT438930.21930714271867108No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG431290.21548989037690672No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG410330.2050174284549981No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG386640.19318094835337524No Hit
CAACAGACCGCCCAACCCCGTCAGCGTCGCCGCCTCGATCAAGAACGAAA338790.1692731571814608No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG336690.16822391242783444No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG322130.16094914880269182No Hit
AGCTCTTTTAGTGTATATTAAATTTGTTGCAGTTAAAAAGCTCGTAGTTG291580.1456851358392229No Hit
GGCTCTAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCT272750.13627690788170674No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG271650.13572730348695008No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG260540.13017629909990788No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTTG260200.1300064213778922No Hit
AGCTCCAATAGTGTATATTAACGTTGCTGCAGTTAAAAAGCTCGTAGTTG260140.12997644295636002No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG259440.12962669470515123No Hit
CCAGGGGCCAATCAAGAACGAAAGTGCCTGACTGACTTACAGATGGCTCA254870.12734333826511676No Hit
CAGTCAATCAAGAACGAAAGTGCCTGACTGACTGTTGGTCAATCTATCTC250120.12497004656048577No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG243760.12179233387807455No Hit
AGCTCTCATAGCGTATATCATCATTGCTGCGGTTAAAAAGCTCGTAGTTG235230.11753040161691614No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG234130.11698079722215948No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCT223390.11161465976789907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA7782100.0293.839979
ATAGCGT6694150.0293.393658
CTCCAAT7904550.0293.18623
AGCTCCA9943000.0293.13821
AATAGCG6653850.0292.78327
CCAATAG7211950.0292.369845
AGCTCTC4778450.0292.26821
GCTCCAA9287400.0292.00782
TCCAATA7931100.0291.892274
GCTCTCA2493900.0288.990882
CTCATGG1474500.0285.096625
TCTCATG1478750.0284.506654
CAATAGC6882800.0282.585426
CTCTCTA2188000.0281.92183
CTAAGTG2189900.0280.889167
CTCTAAG2191250.0280.8445
CTCTCAT2046500.0279.374243
CTCCAGT1018900.0278.728523
TAAGTGT2213050.0278.25088
TAGTGTA843650.0276.834569
Produced by FastQC (version 0.11.2)