##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJJF6 l01n03.333000000349b0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11768177 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.056085577230867 32.0 30.0 32.0 18.0 33.0 2 31.27761275174566 32.0 32.0 33.0 29.0 33.0 3 30.542225104194134 32.0 32.0 33.0 18.0 33.0 4 31.115289734340333 32.0 32.0 33.0 29.0 33.0 5 31.853345339724243 32.0 32.0 33.0 31.0 33.0 6 36.20030876490046 37.0 37.0 37.0 34.0 37.0 7 36.38371185273641 37.0 37.0 37.0 37.0 37.0 8 36.55512140920382 37.0 37.0 37.0 37.0 37.0 9 36.53858214403132 37.0 37.0 37.0 37.0 37.0 10-11 36.44634117926677 37.0 37.0 37.0 37.0 37.0 12-13 36.929177688268965 37.5 37.5 37.5 37.0 37.5 14-15 36.70571610199269 38.0 37.5 38.0 36.0 38.0 16-17 36.06547696384921 38.0 37.5 38.0 34.0 38.0 18-19 37.384198716589665 38.0 38.0 38.0 36.5 38.0 20-21 36.32610858079378 38.0 37.5 38.0 33.5 38.0 22-23 36.50148667886283 38.5 37.5 38.5 33.0 38.5 24-25 36.58971304561446 38.5 37.0 39.0 32.5 39.0 26-27 36.14376950652594 38.0 37.0 39.0 32.0 39.0 28-29 37.87176072385723 39.0 38.0 39.0 37.0 39.0 30-31 36.14191361159847 38.5 37.0 39.0 27.0 39.0 32-33 35.24943769965391 37.5 35.5 39.0 20.0 39.0 34-35 35.90977153895629 38.0 36.5 39.0 32.0 39.0 36-37 36.0183282848312 38.5 37.0 39.0 32.0 39.0 38-39 36.34338963460526 39.0 37.0 39.0 32.5 39.0 40-41 35.814588019877675 38.0 36.5 39.0 32.0 39.0 42-43 36.49362348135995 38.0 36.5 39.0 33.5 39.0 44-45 35.83169058385169 38.5 36.5 38.5 31.5 39.0 46-47 36.15226861390681 39.0 37.0 39.0 32.0 39.0 48-49 35.83790063660667 38.5 36.5 39.0 26.0 39.0 50-51 35.85641314708302 38.0 37.0 39.0 32.0 39.0 52-53 36.101711887916025 38.0 37.0 39.0 32.0 39.0 54-55 36.03643236331337 38.0 36.5 39.0 32.5 39.0 56-57 36.282492649456245 38.5 37.0 39.0 32.5 39.0 58-59 35.528552128337296 38.0 36.5 39.0 31.0 39.0 60-61 35.19906235264816 38.0 36.0 39.0 25.0 39.0 62-63 35.78461111691301 38.0 36.0 39.0 32.0 39.0 64-65 35.97510519258845 38.0 37.0 39.0 32.0 39.0 66-67 35.86306009843326 38.0 37.0 39.0 31.0 39.0 68-69 35.8199513399569 38.0 37.0 39.0 30.5 39.0 70-71 35.55927604589904 38.0 36.5 39.0 25.5 39.0 72-73 35.3914126631508 38.0 36.0 39.0 24.5 39.0 74-75 35.49365895839262 38.0 36.0 39.0 25.0 39.0 76-77 35.78160491637745 38.0 37.0 39.0 30.0 39.0 78-79 35.908508811517706 38.0 37.0 39.0 30.0 39.0 80-81 35.763406388262176 38.0 37.0 39.0 29.5 39.0 82-83 35.142992325829226 38.0 36.0 39.0 19.0 39.0 84-85 35.525976113377624 38.0 36.5 39.0 24.5 39.0 86-87 35.902515359855656 38.0 37.0 39.0 30.5 39.0 88-89 36.014854892138345 38.0 37.0 39.0 30.5 39.0 90-91 35.78802434735643 38.0 37.0 38.5 30.5 39.0 92-93 35.77931089071825 38.0 36.5 38.5 29.5 39.0 94-95 35.66881276513771 38.0 36.5 39.0 24.0 39.0 96-97 35.565431927137055 38.0 36.0 39.0 29.0 39.0 98-99 35.859218679324755 38.0 37.0 39.0 30.0 39.0 100-101 34.989712807684654 38.0 35.0 39.0 23.5 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.14155605548536698 1101 2 -0.10692492292875144 1101 3 0.5040642550518051 1101 4 0.3096019637911809 1101 5 -0.012843197216668045 1101 6 -0.006116693384079497 1101 7 0.005368794810628685 1101 8 0.022734340963516786 1101 9 0.1292567007701777 1101 10-11 -0.016920095836837845 1101 12-13 0.027134560454094014 1101 14-15 0.810939064977191 1101 16-17 0.8457962384969449 1101 18-19 0.17227119117317358 1101 20-21 0.28936633413697876 1101 22-23 0.24486217113388165 1101 24-25 0.31049601863745835 1101 26-27 0.04001701512820688 1101 28-29 0.017079384041082335 1101 30-31 0.04662751597633985 1101 32-33 -0.25066839877857916 1101 34-35 0.2278342798407067 1101 36-37 0.2662878558715178 1101 38-39 0.09369254858587084 1101 40-41 0.3482850013490477 1101 42-43 -0.1198212874512663 1101 44-45 -0.37251479584798375 1101 46-47 0.48310407773495 1101 48-49 0.15416637267363598 1101 50-51 0.37868450872533543 1101 52-53 0.5102563672962788 1101 54-55 0.4921546350097401 1101 56-57 0.1370135523752296 1101 58-59 0.5078715269041751 1101 60-61 0.3017605922179527 1101 62-63 0.12141725616525179 1101 64-65 0.48776117724033696 1101 66-67 0.0956112159913971 1101 68-69 0.23416302284017831 1101 70-71 0.28813991982779186 1101 72-73 0.0908769777480174 1101 74-75 0.05739762200159504 1101 76-77 0.09969397769476274 1101 78-79 0.24593293654839954 1101 80-81 0.20672079950885092 1101 82-83 0.7901511069390423 1101 84-85 0.3181423540252766 1101 86-87 0.14738015922604575 1101 88-89 0.19045188500717103 1101 90-91 0.2396708760758841 1101 92-93 0.35393402878004565 1101 94-95 0.5149974454087172 1101 96-97 0.4220714573815272 1101 98-99 0.09359702471967069 1101 100-101 -0.16199842267419484 1102 1 -0.22245867760976878 1102 2 -0.11907518113241977 1102 3 0.20967791307838723 1102 4 0.13757386251258907 1102 5 -0.02047394082500631 1102 6 -0.05263800672333474 1102 7 -0.007813729429763328 1102 8 -0.0556756165127581 1102 9 0.11544697415060057 1102 10-11 0.0032735374950121354 1102 12-13 0.03618875730885662 1102 14-15 0.8082927635370964 1102 16-17 0.8853279337080551 1102 18-19 0.11358402993436556 1102 20-21 0.23270308764309533 1102 22-23 -0.11672040922208993 1102 24-25 0.24190438817395687 1102 26-27 0.17055045559071402 1102 28-29 0.19541107967221905 1102 30-31 0.1310764585474118 1102 32-33 -0.1552787622449685 1102 34-35 0.13274015813835405 1102 36-37 -0.1590551458423093 1102 38-39 0.10463385191061292 1102 40-41 0.06548413391498542 1102 42-43 -0.2301641772800167 1102 44-45 -0.3990244034899817 1102 46-47 0.34689260992595905 1102 48-49 -0.2569513654847313 1102 50-51 -0.2680930474505985 1102 52-53 -0.11459092314355956 1102 54-55 0.08109437248319296 1102 56-57 -0.41025465528691996 1102 58-59 0.2898038615577363 1102 60-61 0.23336855586884298 1102 62-63 0.15340822255213027 1102 64-65 0.0066524782920325265 1102 66-67 0.08636537974702918 1102 68-69 0.1076780470683758 1102 70-71 0.05499829615092722 1102 72-73 -0.14926752647729558 1102 74-75 -0.10407872455898115 1102 76-77 -0.0866495677215866 1102 78-79 0.15036521085966825 1102 80-81 0.1748396112665347 1102 82-83 0.7104906108168976 1102 84-85 0.29430279141716653 1102 86-87 0.11716097015479932 1102 88-89 0.05597001932820689 1102 90-91 0.06280695456384677 1102 92-93 0.31847307730269847 1102 94-95 0.48834709016967537 1102 96-97 0.29039291121300437 1102 98-99 0.011939055076403804 1102 100-101 -1.4482822458572642 1103 1 -0.05626594783613825 1103 2 -0.12180878955308572 1103 3 0.005436413822621944 1103 4 0.10019475809850675 1103 5 -0.10522198798445004 1103 6 -0.01219706508848617 1103 7 0.041335365863169216 1103 8 0.07391961142269565 1103 9 0.15328078745906026 1103 10-11 0.13834487299511267 1103 12-13 0.11423263046751941 1103 14-15 0.873526259352694 1103 16-17 0.737937892340021 1103 18-19 0.11283687279287591 1103 20-21 0.35480955112728907 1103 22-23 0.37663135324658725 1103 24-25 -0.48953650705666973 1103 26-27 -0.14177559717855814 1103 28-29 0.023521250007952688 1103 30-31 -0.48265746384689834 1103 32-33 -0.11101515013465502 1103 34-35 0.040074405838367966 1103 36-37 -0.7641087177459553 1103 38-39 0.021572101170541202 1103 40-41 0.0036551956764014903 1103 42-43 -0.04457149036890229 1103 44-45 -0.28837998297311884 1103 46-47 0.42077266898418486 1103 48-49 -0.16404014299056513 1103 50-51 0.1853326813370515 1103 52-53 0.024671187104161163 1103 54-55 0.19704274733447846 1103 56-57 -0.16059084012318436 1103 58-59 0.07347051411714034 1103 60-61 0.17383891885148728 1103 62-63 0.06034662738149166 1103 64-65 0.32687448376680805 1103 66-67 0.05532033405223302 1103 68-69 0.19285134095018464 1103 70-71 -0.04639867132489428 1103 72-73 -0.17233260384963245 1103 74-75 -0.17729733338171627 1103 76-77 0.03442130301977642 1103 78-79 0.16125982634915204 1103 80-81 0.2501281131551565 1103 82-83 0.6117339809195457 1103 84-85 0.23689757487478147 1103 86-87 0.2766501736996929 1103 88-89 0.04309002002954543 1103 90-91 -0.19026254084291594 1103 92-93 0.25075650046483133 1103 94-95 0.1074854705007553 1103 96-97 0.2147812939659417 1103 98-99 0.010700545138639939 1103 100-101 -0.7435139183924449 2112 1 -0.5237502532818787 2112 2 -0.6522136944956962 2112 3 -0.8339727818114255 2112 4 -0.6817755462530961 2112 5 -0.5199156324373142 2112 6 -0.3362666883638994 2112 7 -0.20535776461800026 2112 8 -0.1462115844281513 2112 9 -0.0404581398985826 2112 10-11 -0.2532413141576413 2112 12-13 -0.10273846131477882 2112 14-15 0.5827161386899888 2112 16-17 0.6108337960064247 2112 18-19 -0.11366642716381392 2112 20-21 -0.19658686393777458 2112 22-23 -0.3156819287485675 2112 24-25 -0.2676957883181217 2112 26-27 -0.8415511887837539 2112 28-29 -0.38987763260249864 2112 30-31 -1.3368470097737273 2112 32-33 -2.1447985383859276 2112 34-35 -1.4204401989019217 2112 36-37 -0.5584338255735304 2112 38-39 -0.9848303706729524 2112 40-41 -1.0536705067239112 2112 42-43 -0.9425960423114006 2112 44-45 -2.2538519764710188 2112 46-47 -0.6024034575183776 2112 48-49 -1.2940551216964522 2112 50-51 -0.9788359821320967 2112 52-53 -1.369088611047701 2112 54-55 -1.678040057610744 2112 56-57 -1.5577849003177775 2112 58-59 -1.1304947650951576 2112 60-61 -1.2621532237219242 2112 62-63 -1.4729210015111676 2112 64-65 -1.3026578031933909 2112 66-67 -2.1566607815926417 2112 68-69 -2.5398417312283783 2112 70-71 -2.390381820577417 2112 72-73 -1.8436129015019773 2112 74-75 -1.977249431206367 2112 76-77 -1.3732413124286538 2112 78-79 -0.8249546104443795 2112 80-81 -1.0844650063425973 2112 82-83 -0.8069276015368345 2112 84-85 -1.485288683323624 2112 86-87 -1.1231918423741973 2112 88-89 -1.5196870191711085 2112 90-91 -1.4532443401476982 2112 92-93 -1.174519049886726 2112 94-95 -0.9832792130301655 2112 96-97 -1.4552441283079034 2112 98-99 -1.5936041563284178 2112 100-101 -1.3684745012472703 1113 1 -0.7119192034211501 1113 2 -0.6190444721664186 1113 3 -1.4582130210176842 1113 4 -0.8157921653345852 1113 5 -0.6503084727536717 1113 6 -0.5465926285051168 1113 7 -0.2960826389691462 1113 8 -0.2758466457107289 1113 9 -0.17602288028481183 1113 10-11 -0.4083972576556292 1113 12-13 -0.27474298977172396 1113 14-15 0.40966428413603495 1113 16-17 0.44853065168326367 1113 18-19 -0.1312093492147639 1113 20-21 -0.3537043398277291 1113 22-23 -0.59538826091309 1113 24-25 -0.5047798365223741 1113 26-27 -1.156545497977369 1113 28-29 -0.4903359894492425 1113 30-31 -1.618668129457717 1113 32-33 -2.427277008654066 1113 34-35 -1.7913470351289575 1113 36-37 -1.4234412501087377 1113 38-39 -1.6860044166660018 1113 40-41 -1.8662775213957232 1113 42-43 -1.418211333023656 1113 44-45 -1.5095591493937235 1113 46-47 -1.2071956349561503 1113 48-49 -1.8501930144432706 1113 50-51 -1.8995031959802375 1113 52-53 -1.9703685820008232 1113 54-55 -1.4453556680606 1113 56-57 -1.671643121505852 1113 58-59 -1.4045670649695339 1113 60-61 -1.8072162709223036 1113 62-63 -1.732729041228012 1113 64-65 -1.8343966201739121 1113 66-67 -1.8055493283202608 1113 68-69 -2.140104968543014 1113 70-71 -2.559152030392724 1113 72-73 -2.0927608230523376 1113 74-75 -2.3327820533179917 1113 76-77 -1.6314399163442843 1113 78-79 -1.619833976242539 1113 80-81 -1.6583712373678097 1113 82-83 -1.5118508167207665 1113 84-85 -1.7754486967869951 1113 86-87 -1.4681209491028113 1113 88-89 -1.834849008537752 1113 90-91 -1.5021659237728855 1113 92-93 -1.7605949935639273 1113 94-95 -1.1493916967884488 1113 96-97 -1.7839010867276102 1113 98-99 -1.5046537397429987 1113 100-101 -1.628919588574881 2113 1 -1.0093270233533786 2113 2 -0.83250935923963 2113 3 -1.3950076465339052 2113 4 -0.6716940698998215 2113 5 -0.5517096698786617 2113 6 -0.35042983270362527 2113 7 -0.11457865901019204 2113 8 -0.08051601644977069 2113 9 0.01041304666222942 2113 10-11 -0.1543202337181313 2113 12-13 -0.083728909604595 2113 14-15 0.6049908555209242 2113 16-17 0.655439947916463 2113 18-19 0.052392287545373506 2113 20-21 -0.45192277757931976 2113 22-23 -0.5417221134974426 2113 24-25 -0.46058237835826077 2113 26-27 -1.2756904541478988 2113 28-29 -0.454222203363976 2113 30-31 -1.1581300462540227 2113 32-33 -2.138460220506424 2113 34-35 -1.7614457836534712 2113 36-37 -1.2465916098088883 2113 38-39 -1.4990635234972913 2113 40-41 -1.293963967912923 2113 42-43 -1.3251581811807327 2113 44-45 -1.4674039738563422 2113 46-47 -1.6405102892943404 2113 48-49 -1.6490127764028202 2113 50-51 -1.6975181433987032 2113 52-53 -1.9880920737597236 2113 54-55 -1.7367290433267897 2113 56-57 -1.618873396472246 2113 58-59 -1.3277579813366387 2113 60-61 -1.7522691676566495 2113 62-63 -2.291629944506802 2113 64-65 -2.0465554234966916 2113 66-67 -1.3094258298448764 2113 68-69 -1.3806276922071063 2113 70-71 -2.212721650437068 2113 72-73 -2.047788089622827 2113 74-75 -2.0981934910198063 2113 76-77 -1.5436620549377622 2113 78-79 -0.9524603971251508 2113 80-81 -1.3094366029021671 2113 82-83 -1.3996876241164387 2113 84-85 -1.4980856062850165 2113 86-87 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-0.4841752498575431 2109 46-47 -0.1707613225404998 2109 48-49 -0.5563531089609413 2109 50-51 -0.7350936945581168 2109 52-53 -0.3603801642799951 2109 54-55 -0.3104135634413723 2109 56-57 -0.5076765099268741 2109 58-59 -0.29492319919231846 2109 60-61 -0.5760691781262182 2109 62-63 -0.6589684856528564 2109 64-65 -0.5576773753459321 2109 66-67 -0.44919159183022117 2109 68-69 -0.4625765214294475 2109 70-71 -0.4789174456194587 2109 72-73 -0.48447984008678446 2109 74-75 -0.5479579756721336 2109 76-77 -0.042296441314100264 2109 78-79 -0.22005409412452792 2109 80-81 -0.34626051196741514 2109 82-83 -0.2952106265744874 2109 84-85 -0.4848586896194007 2109 86-87 -0.34436729371198993 2109 88-89 -0.526042341946102 2109 90-91 -0.25146236521643317 2109 92-93 -0.3872798617979072 2109 94-95 -0.03932738336174424 2109 96-97 -0.38825495763934725 2109 98-99 -0.5592322717639249 2109 100-101 -0.47840667803392734 2108 1 -1.7236507223122324 2108 2 -0.5558613493257667 2108 3 -2.458944298084276 2108 4 -2.3713707855739976 2108 5 -0.5939763846625219 2108 6 -0.18168060656513774 2108 7 -0.06347335399423315 2108 8 -0.05173849928512908 2108 9 -2.7088268230237915 2108 10-11 -0.45484204408435147 2108 12-13 -0.07837122519787698 2108 14-15 0.6660424336191184 2108 16-17 0.8057356654009808 2108 18-19 0.06135386135493803 2108 20-21 -2.0091647980640275 2108 22-23 -2.265704384937848 2108 24-25 -2.44781908252002 2108 26-27 -1.987648737546344 2108 28-29 -0.3961821464791271 2108 30-31 -2.8649153737805264 2108 32-33 -3.32969283022841 2108 34-35 -3.2355833655148487 2108 36-37 -2.438576681979697 2108 38-39 -2.612360725452426 2108 40-41 -3.6917333521479136 2108 42-43 -1.5312399709912583 2108 44-45 -1.455889495294791 2108 46-47 -1.8725707250848984 2108 48-49 -3.11159265469324 2108 50-51 -3.2406077643301856 2108 52-53 -2.696310498545124 2108 54-55 -1.2109933943759046 2108 56-57 -1.7353857733375264 2108 58-59 -2.1552070793971225 2108 60-61 -2.083285896760245 2108 62-63 -1.5959300922171096 2108 64-65 -3.2728082781075685 2108 66-67 -1.4024054607769827 2108 68-69 -2.0391329501026547 2108 70-71 -2.1219765378939286 2108 72-73 -2.2217963164379313 2108 74-75 -2.3215633148623027 2108 76-77 -1.446068618153042 2108 78-79 -2.1427027039178483 2108 80-81 -2.578396202358171 2108 82-83 -2.414094175283637 2108 84-85 -1.990965087926405 2108 86-87 -1.9633524464585363 2108 88-89 -1.8680893716382272 2108 90-91 -0.9807263743884889 2108 92-93 -1.6156501156773544 2108 94-95 -1.1023383792970165 2108 96-97 -1.5090325904979096 2108 98-99 -0.9251101030867162 2108 100-101 -2.4829460044169522 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 12.0 14 9.0 15 46.0 16 242.0 17 972.0 18 4370.0 19 13051.0 20 18885.0 21 16900.0 22 16544.0 23 23395.0 24 38268.0 25 59766.0 26 88457.0 27 126649.0 28 171853.0 29 221691.0 30 279686.0 31 345073.0 32 416987.0 33 527162.0 34 699780.0 35 994990.0 36 1665026.0 37 4134024.0 38 1904339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 71.53304203361319 20.011672156188677 6.34440661455041 2.11087919564772 2 90.36343358252236 2.613642302226131 1.6799036384146224 5.343020476836893 3 9.395848600496798 39.53060355317595 36.51226834133004 14.561279504997207 4 1.7279481775299605 23.971818234888886 72.36644214307789 1.9337914445032565 5 1.3533873048805525 95.34448669020585 1.5975627828815613 1.7045632220320295 6 1.2765190864343876 1.6813567099401565 1.5401113596628193 95.50201284396263 7 1.3234252549779384 1.5084496958959228 1.5290901095539453 95.6390349395722 8 1.3216576638554693 1.6518192793853526 95.54484059381845 1.481682462940731 9 1.2470836602035862 1.5391170220431782 95.78883762445429 1.4249616932989446 10-11 48.19633696010593 1.593037799866445 48.900188572120754 1.310436667906876 12-13 2.892635792272669 48.42276335578569 47.20432909872107 1.480271753220571 14-15 48.84458551144725 1.4903290881269051 1.8432954254013554 47.82178997502449 16-17 1.3350836859637145 46.17797820533615 1.4833755988398356 51.0035625098603 18-19 1.2694618095446566 1.4424070476172348 95.84735986273488 1.4407712801032206 20-21 3.2196150686720637 30.908848498794672 34.22559415957119 31.645942272962074 22-23 10.599487924085437 7.411028912974372 10.788217240444293 71.20126592249589 24-25 1.223579488989671 3.297656043072772 37.45782800513622 58.02093646280133 26-27 34.3795517351583 26.093493495211707 4.771575920382571 34.75537884924743 28-29 1.1708696402908998 1.7246130581323733 55.525971059576264 41.578546242000456 30-31 7.853676062146244 43.174393111184514 34.810174931937205 14.161755894732039 32-33 35.25608086961982 47.72439265656864 9.678580633177084 7.34094584063445 34-35 20.38166574143132 30.443538536172593 35.049489823275096 14.125305899120994 36-37 5.093775573471387 35.52866920376322 28.079159077874127 31.298396144891267 38-39 11.7134540039634 51.10689616582076 12.643253921146835 24.536395909069007 40-41 32.921811084248645 16.283452398787 6.895592239987552 43.8991442769768 42-43 22.27867153935567 8.356260277186516 66.079108939303 3.2859592441548084 44-45 52.34867728451059 18.893635777232106 20.440846530435426 8.316840407821873 46-47 8.134722140905936 44.707383310091274 33.7108457835058 13.447048765496984 48-49 19.2423856303317 37.42452633062878 27.064417029077653 16.268671009961867 50-51 28.89655721527642 19.278780392239174 19.060445810765763 32.76421658171865 52-53 7.298997967144784 4.039448930790215 55.45883614768881 33.20271695437619 54-55 4.606180915114589 6.9957822267536525 38.176084459644194 50.221952398487566 56-57 13.849180457142191 17.801215846822146 11.171012773304344 57.17859092273132 58-59 20.687698697937666 9.504560476954078 32.32040952477176 37.48733130033649 60-61 19.051565930729968 46.53108123713639 16.92355578948209 17.49379704265155 62-63 44.40590076100997 18.282649045812278 16.68976001975497 20.62169017342278 64-65 15.387553229357446 23.8283635604733 47.666439755282404 13.117643454886855 66-67 21.95467913169559 37.439418186861054 18.347744089845012 22.25815859159834 68-69 25.035334699673534 28.56965441631274 8.63030867057829 37.764702213435434 70-71 15.080096942797514 32.74588748962562 27.987546414368175 24.18646915320869 72-73 13.186876777941054 28.362850932646577 20.26254788655881 38.18772440285356 74-75 15.042682481747171 44.09859318057504 25.406105805512613 15.452618532165177 76-77 17.77133790560764 13.214149481266299 29.920849253032138 39.09366336009392 78-79 9.721178977898573 16.960550345246777 33.365878732359256 39.952391944495396 80-81 30.269713822285304 16.320854113598056 35.476331635732535 17.93310042838411 82-83 25.281205406750768 14.368937516830346 38.295098722597395 22.054758353821498 84-85 27.5247772021104 20.477360257242903 22.89215653367552 29.10570600697117 86-87 10.500700320873827 49.217402151582185 14.735659567322958 25.546237960221024 88-89 14.589502690178776 13.951098798055128 13.15210503716931 58.30729347459679 90-91 13.874090269036571 32.24392359156393 13.06434293093994 40.81764320845956 92-93 11.304801074966836 14.676257843504564 33.79421043718156 40.22473064434704 94-95 13.092699064604485 16.47977847376021 13.346655136135361 57.08086732549995 96-97 14.142195267040735 30.08899042260286 19.953341964237197 35.815472346119215 98-99 35.22012797734092 12.961298083806863 38.59079872778936 13.227775211062854 100-101 28.282349934063706 17.529082881741157 36.52423395739204 17.664333226803098 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 64.0 1 34.5 2 3.0 3 1.5 4 3.0 5 2.5 6 1.5 7 2.5 8 3.5 9 3.5 10 4.0 11 6.5 12 5.5 13 4.0 14 6.0 15 7.5 16 13.5 17 23.5 18 29.5 19 40.0 20 76.5 21 130.0 22 188.5 23 314.0 24 609.0 25 1855.5 26 3490.0 27 7092.5 28 7537.5 29 10545.0 30 13793.5 31 11983.5 32 20880.0 33 54101.5 34 69926.0 35 60837.5 36 61066.0 37 210436.0 38 262092.5 39 168484.5 40 167822.5 41 164798.0 42 239977.0 43 457667.0 44 606108.0 45 514365.5 46 533243.5 47 648308.0 48 636438.5 49 659324.5 50 968203.0 51 1689950.5 52 1376623.5 53 468754.5 54 449345.0 55 417641.5 56 211152.5 57 173531.0 58 137967.5 59 85238.0 60 57441.0 61 41789.5 62 35536.5 63 30002.0 64 20824.5 65 4260.0 66 1705.5 67 993.5 68 640.5 69 440.5 70 305.5 71 206.5 72 157.0 73 131.5 74 103.0 75 76.5 76 49.5 77 41.5 78 47.5 79 40.0 80 22.5 81 23.0 82 26.5 83 30.5 84 36.0 85 28.5 86 20.0 87 24.0 88 31.5 89 29.0 90 26.0 91 26.0 92 24.0 93 24.5 94 27.5 95 31.0 96 31.0 97 31.0 98 35.5 99 89.0 100 355.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.6994985714439885E-5 3 8.497492857219942E-6 4 0.0 5 8.497492857219942E-6 6 1.6994985714439885E-5 7 1.6994985714439885E-5 8 8.497492857219942E-6 9 8.497492857219942E-6 10-11 4.248746428609971E-6 12-13 0.0 14-15 1.2644014446757557 16-17 2.2677726550170005 18-19 8.497492857219942E-6 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 8.497492857219942E-6 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.248746428609971E-6 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.248746428609971E-6 56-57 4.248746428609971E-6 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.248746428609971E-6 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 4.248746428609971E-6 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1768177E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.743931140305824 #Duplication Level Percentage of deduplicated Percentage of total 1 84.17551496859387 9.043759365223545 2 6.5634197393087375 1.4103385944811415 3 2.6539995397458958 0.8554316490429975 4 1.4631463587793603 0.6287977490765858 5 0.7222126993204704 0.38797017550767643 6 0.5286071421467743 0.3407591241299277 7 0.35839925237504744 0.26954318217782186 8 0.34579669604745156 0.2972172712703266 9 0.20517779174250703 0.19839744594013536 >10 2.4251622815636598 5.968990561840244 >50 0.29285729826382195 2.1195973353540962 >100 0.1955620907209118 4.414259734703108 >500 0.030368182124880697 2.2730113400110734 >1k 0.03019750694807275 6.954171281038528 >5k 0.0036221044592758423 2.752779843594652 >10k+ 0.005956347859038062 62.0849753466082 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 1665934 14.156262265599844 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC 585254 4.973191684659399 No Hit AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 466286 3.962261954421658 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 396013 3.3651176388662405 No Hit TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG 355400 3.0200089614559675 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG 341369 2.9007806391763142 No Hit GGATACCTTGTTACGACTTAGTCCCAATCAGTGGTTTTACCTTAGAGCCC 247134 2.100019399776193 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT 234786 1.995092357975241 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT 195817 1.6639535588222374 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA 182911 1.5542849160069567 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA 173387 1.473354794034794 No Hit GGAAACCTTGTTACGCCTTTTCCTTCCTCTAAGTGATATGACTTACAAAA 173143 1.4712814057776322 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA 169502 1.4403420342844946 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 154982 1.3169584379976609 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC 113885 0.9677369740444931 No Hit GGATACCTTGTGACGACTTAACCTACCTTGTCTTACAAAGGTTCGACCCT 103961 0.8834078549294423 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT 87667 0.7449497063139007 No Hit GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA 81376 0.69149197874913 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 78514 0.6671721541917665 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA 75132 0.6384336333486487 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA 74409 0.6322899460128787 No Hit AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 70447 0.5986228793125732 No Hit GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC 69355 0.5893436171124891 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT 53563 0.45515120991127167 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC 43895 0.37299744896766934 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC 42180 0.35842424871753714 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATATGGTTTACTTAT 40631 0.3452616322817034 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATG 40489 0.34405498829597825 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCTACAGTTTGGTCGT 40396 0.34326472146025677 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA 37125 0.3154694223242903 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA 33738 0.2866884140168864 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA 30064 0.25546862525946035 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAAGC 29303 0.24900203319511596 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA 27454 0.23329016890211626 No Hit GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA 25015 0.21256478382335686 No Hit GGATACCTTGTTACGACTTAGCCCCCCTCACCTATCTCTGGTTCGACAGC 24334 0.20677799118759005 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 23930 0.2033450040732732 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA 23833 0.20252074726612285 No Hit AGATACCTTGTGACGACTTAACCTACCTTGTCGTGAAAAGGTTCTTCCCA 22969 0.19517891343748484 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGCTTTACATAT 22495 0.19115110182316258 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC 22435 0.1906412522517294 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTACACTTTTAGATTTAAAAAA 20691 0.1758216247087378 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT 20248 0.17205723537298936 No Hit AGATACCTTGTGACGACTTAACCTACCTTGTCATGAAAAGACTCTTCTCT 19756 0.16787646888723717 No Hit GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC 19494 0.16565012575864554 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGTCAAGGTTTACTTAA 19406 0.16490234638721019 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAGCGCTACAGTTTGGTCGT 18579 0.1578749197942893 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCACTAAA 18464 0.156897708115709 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGGCCAAGTTTGTCCAA 17839 0.15158677507994653 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC 17732 0.150677543344224 No Hit AGATACCTTGTTACGACTTAACCCTCCTTGCTGAATCTCAGTTCGAAGAC 17683 0.15026116619422022 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC 16784 0.1426219201155795 No Hit GGATACCTTGTTACGACTTCATCCCAGTCACCGATATCACCTTAGGCGTC 16411 0.13945235527983646 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 16203 0.13768487676553473 No Hit AGATACCTTGTTACGACTTAACCCCCCTTGCTGAACCCAAGTTCTAACGC 16066 0.13652072024409556 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA 15099 0.1283036446511639 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG 15043 0.12782778505115958 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC 14752 0.12535501462970858 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC 14543 0.12357903862254961 No Hit TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAATC 14424 0.12256783697254044 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGATTTAAATAA 14314 0.12163311275824623 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAGC 14263 0.12119974062252803 No Hit GGAAACCTTGTTACGACTTCTGCTTCCTCCAAGTGATAAGGTTCACAAAG 13070 0.11106223164386464 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA 13065 0.11101974417957854 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA 12938 0.1099405625867116 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA 12705 0.10796064675097936 No Hit AGATACCTTGTGACGACTTAACCTACCTTGTCATGAAAAGGTTTTTCCCA 12698 0.10790116430097882 No Hit AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAAC 12252 0.10411128248665873 No Hit TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC 12097 0.10279417109378963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.3792980000215836E-4 0.0 0.0 2 2.3792980000215836E-4 0.0 0.0 3 2.3792980000215836E-4 0.0 0.0 4 2.3792980000215836E-4 0.0 0.0 5 2.3792980000215836E-4 0.0 0.0 6 2.464272928593783E-4 0.0 0.0 7 2.5492478571659824E-4 0.0 0.0 8 2.5492478571659824E-4 0.0 0.0 9 2.634222785738182E-4 0.0 0.0 10-11 2.88914757145478E-4 0.0 0.0 12-13 2.88914757145478E-4 0.0 0.0 14-15 2.9741225000269795E-4 0.0 0.0 16-17 2.9741225000269795E-4 0.0 0.0 18-19 3.0166099643130794E-4 0.0 0.0 20-21 3.3140222143157773E-4 0.0 0.0 22-23 3.4414846071740765E-4 0.0 0.0 24-25 3.653921928604575E-4 0.0 0.0 26-27 3.908846714321173E-4 0.0 0.0 28-29 3.9513341786072723E-4 8.49749285721994E-6 0.0 30-31 4.206258964323871E-4 8.49749285721994E-6 0.0 32-33 4.5886461428987685E-4 8.49749285721994E-6 0.0 34-35 4.801083464329267E-4 8.49749285721994E-6 0.0 36-37 5.013520785759765E-4 8.49749285721994E-6 0.0 38-39 5.183470642904165E-4 8.49749285721994E-6 0.0 40-41 5.650832750051262E-4 8.49749285721994E-6 0.0 42-43 6.245657250056656E-4 8.49749285721994E-6 0.0 44-45 6.670531892917653E-4 8.49749285721994E-6 0.0 46-47 7.052919071492552E-4 8.497492857219941E-5 0.0 48-49 7.435306250067449E-4 8.497492857219941E-5 0.0 50-51 8.285055535789443E-4 8.497492857219941E-5 0.0 52-53 9.049829892939238E-4 8.497492857219941E-5 0.0 54-55 9.559679464372434E-4 8.497492857219941E-5 0.0 56-57 0.0010834303392955424 8.497492857219941E-5 0.0 58-59 0.0011981464928680118 8.497492857219941E-5 0.0 60-61 0.0012873701678688212 8.497492857219941E-5 0.0 62-63 0.0014020863214412903 8.497492857219941E-5 0.0 64-65 0.0015040562357279295 8.497492857219941E-5 0.0 66-67 0.0016060261500145689 8.497492857219941E-5 0.0 68-69 0.001741986035730088 8.497492857219941E-5 0.0 70-71 0.0019374283714461466 8.497492857219941E-5 0.0 72-73 0.002124373214304986 8.497492857219941E-5 0.0 74-75 0.002311318057163824 8.497492857219941E-5 0.0 76-77 0.0025619940964518124 8.497492857219941E-5 0.0 78-79 0.002753187685739261 8.497492857219941E-5 0.0 80-81 0.0029826199928841995 8.497492857219941E-5 0.0 82-83 0.0032587885107438477 8.497492857219941E-5 0.0 84-85 0.003662419421461795 8.497492857219941E-5 0.0 86-87 0.004015065375036422 8.497492857219941E-5 0.0 88-89 0.0043889550607541 8.497492857219941E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTACG 1029775 0.0 96.88725 9 TTGTTAC 1028950 0.0 96.866455 8 TTGTGAC 35955 0.0 96.620316 8 CTTGTTA 1054870 0.0 94.43917 7 CCTTGTT 1057100 0.0 94.39149 6 GATACCT 181100 0.0 94.35193 2 GTTACCT 401820 0.0 94.25892 2 AAACCTT 249720 0.0 94.22772 3 AGATACC 101185 0.0 94.006195 1 GGATACC 73885 0.0 93.93029 1 TCGTACC 45670 0.0 93.89543 1 CTTGTGA 37195 0.0 93.857574 7 AACCTTG 262930 0.0 93.727295 4 GGTACCT 47740 0.0 93.59526 2 GGTTACC 401710 0.0 93.387215 1 ATACCTT 184370 0.0 93.378174 3 CCTTGTG 37745 0.0 93.35374 6 GGAAACC 248195 0.0 93.29883 1 GTACCTT 95590 0.0 92.958626 3 CGTACCT 47305 0.0 92.897644 2 >>END_MODULE