FastQCFastQC Report
Fri 25 Sep 2015
000000000-AJJF6 l01n03.333000000349b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJJF6 l01n03.333000000349b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11768177
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC166593414.156262265599844No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC5852544.973191684659399No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT4662863.962261954421658No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC3960133.3651176388662405No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG3554003.0200089614559675No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG3413692.9007806391763142No Hit
GGATACCTTGTTACGACTTAGTCCCAATCAGTGGTTTTACCTTAGAGCCC2471342.100019399776193No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT2347861.995092357975241No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT1958171.6639535588222374No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA1829111.5542849160069567No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA1733871.473354794034794No Hit
GGAAACCTTGTTACGCCTTTTCCTTCCTCTAAGTGATATGACTTACAAAA1731431.4712814057776322No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA1695021.4403420342844946No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT1549821.3169584379976609No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC1138850.9677369740444931No Hit
GGATACCTTGTGACGACTTAACCTACCTTGTCTTACAAAGGTTCGACCCT1039610.8834078549294423No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT876670.7449497063139007No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA813760.69149197874913No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC785140.6671721541917665No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA751320.6384336333486487No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA744090.6322899460128787No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG704470.5986228793125732No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC693550.5893436171124891No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT535630.45515120991127167No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC438950.37299744896766934No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC421800.35842424871753714No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATATGGTTTACTTAT406310.3452616322817034No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATG404890.34405498829597825No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCTACAGTTTGGTCGT403960.34326472146025677No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA371250.3154694223242903No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA337380.2866884140168864No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA300640.25546862525946035No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAAGC293030.24900203319511596No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA274540.23329016890211626No Hit
GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA250150.21256478382335686No Hit
GGATACCTTGTTACGACTTAGCCCCCCTCACCTATCTCTGGTTCGACAGC243340.20677799118759005No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC239300.2033450040732732No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA238330.20252074726612285No Hit
AGATACCTTGTGACGACTTAACCTACCTTGTCGTGAAAAGGTTCTTCCCA229690.19517891343748484No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGCTTTACATAT224950.19115110182316258No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC224350.1906412522517294No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTACACTTTTAGATTTAAAAAA206910.1758216247087378No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT202480.17205723537298936No Hit
AGATACCTTGTGACGACTTAACCTACCTTGTCATGAAAAGACTCTTCTCT197560.16787646888723717No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC194940.16565012575864554No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGTCAAGGTTTACTTAA194060.16490234638721019No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAGCGCTACAGTTTGGTCGT185790.1578749197942893No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCACTAAA184640.156897708115709No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGGCCAAGTTTGTCCAA178390.15158677507994653No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC177320.150677543344224No Hit
AGATACCTTGTTACGACTTAACCCTCCTTGCTGAATCTCAGTTCGAAGAC176830.15026116619422022No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC167840.1426219201155795No Hit
GGATACCTTGTTACGACTTCATCCCAGTCACCGATATCACCTTAGGCGTC164110.13945235527983646No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC162030.13768487676553473No Hit
AGATACCTTGTTACGACTTAACCCCCCTTGCTGAACCCAAGTTCTAACGC160660.13652072024409556No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA150990.1283036446511639No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG150430.12782778505115958No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACAGTGGTAAGC147520.12535501462970858No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC145430.12357903862254961No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAATC144240.12256783697254044No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGATTTAAATAA143140.12163311275824623No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAGC142630.12119974062252803No Hit
GGAAACCTTGTTACGACTTCTGCTTCCTCCAAGTGATAAGGTTCACAAAG130700.11106223164386464No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA130650.11101974417957854No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA129380.1099405625867116No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA127050.10796064675097936No Hit
AGATACCTTGTGACGACTTAACCTACCTTGTCATGAAAAGGTTTTTCCCA126980.10790116430097882No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAAC122520.10411128248665873No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAACC120970.10279417109378963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG10297750.096.887259
TTGTTAC10289500.096.8664558
TTGTGAC359550.096.6203168
CTTGTTA10548700.094.439177
CCTTGTT10571000.094.391496
GATACCT1811000.094.351932
GTTACCT4018200.094.258922
AAACCTT2497200.094.227723
AGATACC1011850.094.0061951
GGATACC738850.093.930291
TCGTACC456700.093.895431
CTTGTGA371950.093.8575747
AACCTTG2629300.093.7272954
GGTACCT477400.093.595262
GGTTACC4017100.093.3872151
ATACCTT1843700.093.3781743
CCTTGTG377450.093.353746
GGAAACC2481950.093.298831
GTACCTT955900.092.9586263
CGTACCT473050.092.8976442
Produced by FastQC (version 0.11.2)