##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJJF6 l01n02.332000000349b3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11768177
Sequences flagged as poor quality	0
Sequence length	12
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19717352993586	33.0	32.0	34.0	18.0	35.0
2	31.38693384710308	33.0	32.0	34.0	16.0	35.0
3	31.524486502879757	33.0	32.0	34.0	18.0	35.0
4	31.46887474585061	33.0	32.0	34.0	18.0	35.0
5	31.474423268786662	33.0	32.0	34.0	18.0	35.0
6	33.50580264045995	37.0	33.0	37.0	19.0	37.0
7	33.29169122796164	35.0	33.0	37.0	18.0	37.0
8	33.0771868064187	35.0	33.0	37.0	18.0	37.0
9	33.795306783710004	37.0	34.0	37.0	18.0	37.0
10	33.44477398665911	37.0	33.0	37.0	18.0	37.0
11	33.479791899798926	36.0	33.0	37.0	29.0	37.0
12	33.09112660355125	35.0	33.0	37.0	18.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.4527795392692866
1101	2	-0.3412309803814182
1101	3	-0.2562894188616518
1101	4	-0.12094645825929007
1101	5	-0.23156668302793548
1101	6	-0.08324742162774612
1101	7	-0.10534741597177799
1101	8	-0.16145617500126974
1101	9	-0.18518494551922515
1101	10	-0.050732286430367424
1101	11	-0.17238627376596583
1101	12	-0.04551244789642794
1102	1	-0.15464759710541998
1102	2	-0.14948746016739634
1102	3	-0.3698861133870608
1102	4	-0.21649016940694565
1102	5	-0.14153175766636394
1102	6	-1.0497915567515435
1102	7	-0.3908501139638574
1102	8	-0.578726525285731
1102	9	-0.4176778019262528
1102	10	-0.17799446386134576
1102	11	-0.12908782545715525
1102	12	-0.03385915435804776
1103	1	-0.07640818010558803
1103	2	-0.11137295119617363
1103	3	-0.12981259971703096
1103	4	-0.1522877912916556
1103	5	-0.0885566561843838
1103	6	0.08959238566986727
1103	7	-0.028948808965843398
1103	8	-0.017234039011270852
1103	9	-0.04695336685746554
1103	10	-0.3475076377002537
1103	11	-0.11687511050809007
1103	12	-0.014920853522134792
2112	1	-0.5187489031735844
2112	2	-0.6419731212295829
2112	3	-0.5480521413452486
2112	4	-0.9688668258888455
2112	5	-0.5547556086807504
2112	6	-0.4300476013512835
2112	7	-0.48372075507764833
2112	8	-0.4457524603485794
2112	9	-0.49494069595258594
2112	10	-1.3669968053222128
2112	11	-0.48563944258923897
2112	12	-0.44339763106393093
1113	1	-0.9330921482155468
1113	2	-0.9836066211752197
1113	3	-1.0230903321364764
1113	4	-1.4217452681736837
1113	5	-1.0251791143715145
1113	6	-0.8337123521067937
1113	7	-0.8499417231727335
1113	8	-0.8194486548335647
1113	9	-0.8576434390744154
1113	10	-1.881730255752398
1113	11	-0.8605067730207949
1113	12	-1.0673371654664514
2113	1	-0.8324462168291511
2113	2	-0.8424786553538759
2113	3	-0.7304132739684803
2113	4	-1.4268127379119449
2113	5	-0.8559102125491371
2113	6	-0.8443893678548875
2113	7	-0.7687937463516761
2113	8	-0.7498614711822
2113	9	-0.7417859811400831
2113	10	-0.7870870645738535
2113	11	-0.47022490749954216
2113	12	-0.7801851228155954
1112	1	-0.31904273304406416
1112	2	-0.3424234451775199
1112	3	-0.2811044404103704
1112	4	-0.18643134849354936
1112	5	-0.4147906827441439
1112	6	-0.1675459438078093
1112	7	-0.32342135039237263
1112	8	-0.3220923003340772
1112	9	-0.27369491309308813
1112	10	-0.1513188858950656
1112	11	-0.27579002261907704
1112	12	-0.21348152198021353
2114	1	-0.19319710814591318
2114	2	-0.2804043630936377
2114	3	-0.16023244169344153
2114	4	-0.13184756136861608
2114	5	-0.19725544739087297
2114	6	-0.0771538860279577
2114	7	-0.2457969756565248
2114	8	-0.1621479340462102
2114	9	-0.2359572288941294
2114	10	0.016169499233605222
2114	11	-0.0976950686202187
2114	12	-0.1467996188726559
1114	1	0.0030175934141354332
1114	2	-0.02113031313032465
1114	3	-0.09265725070121889
1114	4	0.013430083850778374
1114	5	-0.026368160709630217
1114	6	0.09645698658646751
1114	7	-0.01171325342795626
1114	8	0.007641048576239484
1114	9	-0.07594606887522559
1114	10	-0.0856034466716622
1114	11	-0.05814949948083381
1114	12	-0.09909033717936921
1109	1	-0.7584390916134538
1109	2	-0.7747529559349466
1109	3	-0.7512444390303195
1109	4	-1.0832009926745094
1109	5	-0.7286958761674107
1109	6	-0.6954613621605219
1109	7	-0.7150396060230406
1109	8	-0.6908403337260438
1109	9	-0.7855014464558252
1109	10	-1.082664495837932
1109	11	-0.7085580232044677
1109	12	-0.668622957188326
1108	1	-0.3503276746145261
1108	2	-0.3982201708167423
1108	3	-0.4886580399095948
1108	4	-0.4523224839929263
1108	5	-0.37763498732189404
1108	6	-0.28204017334081755
1108	7	-0.34181817704759965
1108	8	-0.32478292149701815
1108	9	-0.3892566207902419
1108	10	-0.35578083948524153
1108	11	-0.32683437800092463
1108	12	-0.370555887625315
1111	1	-0.5486133382589138
1111	2	-0.639652065003645
1111	3	-0.5172346474119855
1111	4	-0.46305467139154644
1111	5	-0.5351791698088242
1111	6	-0.4847899537906315
1111	7	-0.5855107206155452
1111	8	-0.5853914903314177
1111	9	-0.6055472010784158
1111	10	-0.3971515130179739
1111	11	-0.46771929486328645
1111	12	-0.5367115462195571
1110	1	-1.0550921222637655
1110	2	-1.12132807637812
1110	3	-1.0310869411036805
1110	4	-1.326711168613997
1110	5	-1.0208959350187499
1110	6	-0.7209831384025236
1110	7	-0.8933791393701043
1110	8	-1.0497627687099822
1110	9	-0.9960125804597268
1110	10	-1.466629076243926
1110	11	-0.929754236234146
1110	12	-0.8380614742840038
1105	1	-0.05551724245428247
1105	2	-0.05840415278709088
1105	3	-0.0380980658665635
1105	4	0.00635487007499691
1105	5	-0.0308466616481482
1105	6	-0.05677106558289324
1105	7	-0.11888170974483359
1105	8	-0.024846375455013003
1105	9	-0.10901053376660741
1105	10	0.10272514441082592
1105	11	-0.001410152696145417
1105	12	-0.02179104432322987
1104	1	0.751319994115093
1104	2	0.7854734553755982
1104	3	0.8187322687078655
1104	4	0.8502429312501825
1104	5	0.8230797756012613
1104	6	0.8067467011133118
1104	7	0.749454186934571
1104	8	0.7136232616731704
1104	9	0.7252190516914467
1104	10	1.0245470820335996
1104	11	0.42739570031142904
1104	12	0.5564645468918599
1107	1	0.503323556937076
1107	2	0.6383743059015181
1107	3	0.5481060772185664
1107	4	0.6990247083362391
1107	5	0.6248163855855928
1107	6	-0.6415324212795497
1107	7	0.28944202002645625
1107	8	0.3436505031945032
1107	9	0.5099331811437082
1107	10	0.6172239137912428
1107	11	0.5052858619002265
1107	12	0.5048480269719207
1106	1	0.7891112152877859
1106	2	0.8016327564643824
1106	3	0.6955979562635086
1106	4	0.9170862334754979
1106	5	0.8489193973327112
1106	6	0.29274133222009624
1106	7	0.5885243884039824
1106	8	0.5443542255669769
1106	9	0.6338395526209268
1106	10	0.715111085355268
1106	11	0.6175998682117481
1106	12	0.7285895632828172
2103	1	0.5954477602092396
2103	2	0.6730616827681821
2103	3	0.654886162993467
2103	4	0.7942396656894708
2103	5	0.6457910434378498
2103	6	0.6494289978108725
2103	7	0.6712634187672535
2103	8	0.7810797356699695
2103	9	0.720433400589549
2103	10	0.9171412906160441
2103	11	0.620506576955627
2103	12	0.541731657396447
2102	1	0.4508043853361485
2102	2	0.5087849556680482
2102	3	0.5807193373152337
2102	4	0.7725088310742159
2102	5	-0.05165974323201539
2102	6	0.5979669137267223
2102	7	0.528904179874111
2102	8	0.5193409106164566
2102	9	0.6102849556456533
2102	10	0.7098794744273391
2102	11	0.47765600099666017
2102	12	0.630832703969169
2101	1	0.6662733871077435
2101	2	0.7871431637395183
2101	3	0.6068986126103013
2101	4	0.8240990450525523
2101	5	0.7956374539236144
2101	6	0.7974646382614665
2101	7	0.6428529580795939
2101	8	0.7463547165313074
2101	9	0.6812460464796573
2101	10	0.8452470259850173
2101	11	0.538227053316426
2101	12	0.584451386058916
2107	1	0.6508965217639364
2107	2	0.6166038616782252
2107	3	0.7301647031800478
2107	4	0.8528475700204474
2107	5	0.6644338651190544
2107	6	0.6992698741411658
2107	7	0.5840077802045798
2107	8	0.7109902501432543
2107	9	0.5323988502693311
2107	10	0.7825938951675795
2107	11	0.570455886958662
2107	12	0.5040267633935258
2106	1	0.5585074149425644
2106	2	0.5812023816081933
2106	3	0.5882698478532404
2106	4	0.6955345002525455
2106	5	0.34695542761899745
2106	6	0.6412569454434802
2106	7	0.6062854150569024
2106	8	0.6095809938142622
2106	9	0.6275871267837871
2106	10	0.6436128117026669
2106	11	0.5586493376914419
2106	12	0.446210302786163
2105	1	0.6708580265547255
2105	2	0.6995751474199849
2105	3	0.6222091574820396
2105	4	0.6648150016496182
2105	5	0.7072355042428207
2105	6	0.7013482081738474
2105	7	0.5552476661335675
2105	8	0.7234282883230421
2105	9	0.5479252878208527
2105	10	0.8206152361182504
2105	11	0.5216398074112476
2105	12	0.21086442425030327
2104	1	0.5978698026436895
2104	2	0.651813181711578
2104	3	0.6624047362653123
2104	4	0.7676093754418041
2104	5	0.7002699069393934
2104	6	0.6779641463548529
2104	7	0.6845475780381705
2104	8	0.36729999097876487
2104	9	0.5472136887100305
2104	10	0.6915480220411823
2104	11	0.5482417220645672
2104	12	0.6347201070158874
2111	1	0.48187540055139877
2111	2	0.5429739908543105
2111	3	0.4382993838142468
2111	4	0.6423618056087648
2111	5	0.5752527662491858
2111	6	0.572335918797755
2111	7	0.46903384790117286
2111	8	0.5572977543953641
2111	9	0.5497091878116507
2111	10	0.6271771103518304
2111	11	0.481678884690659
2111	12	0.4389125271392942
2110	1	0.4752690094433696
2110	2	0.5099111107098935
2110	3	0.5177128061127263
2110	4	0.639681939200706
2110	5	0.5569543915976709
2110	6	0.49659649585904475
2110	7	0.5218651992003487
2110	8	0.4506151748266731
2110	9	0.41936182590553983
2110	10	0.662440782597713
2110	11	0.2977978065263258
2110	12	0.42854310123978934
2109	1	-0.4732652721440367
2109	2	-0.5628219742318059
2109	3	-0.5403827862027839
2109	4	-0.48267169794265996
2109	5	-0.48439379690171336
2109	6	-0.35987656355532494
2109	7	-0.4191280981945624
2109	8	-0.5246560424075568
2109	9	-0.38573004250930865
2109	10	-0.422050254882528
2109	11	-0.5558866017748443
2109	12	-0.5880161697343453
2108	1	-0.472956901069427
2108	2	-0.5272626878419686
2108	3	-0.5057581180706059
2108	4	-0.7064473855678415
2108	5	-0.5241254242247031
2108	6	-0.39182673651873046
2108	7	-0.6091370446448963
2108	8	-0.6182573621402696
2108	9	-0.5043092890795648
2108	10	-0.6027853481575107
2108	11	-0.508616896700417
2108	12	-0.341852177866663
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	43.0
15	1690.0
16	53161.0
17	110045.0
18	155220.0
19	189498.0
20	175224.0
21	195695.0
22	186084.0
23	160353.0
24	154198.0
25	151428.0
26	144108.0
27	145310.0
28	158940.0
29	173877.0
30	217359.0
31	270822.0
32	438999.0
33	1030267.0
34	3018119.0
35	3923313.0
36	714424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.195326514888414	41.700621939999714	24.78538519602484	14.318666349087033
2	29.993736498014943	28.146806425498188	19.61777087479225	22.24168620169462
3	21.412568828629958	20.520102646314715	23.473814168498656	34.59351435655667
4	36.50255260436685	14.63702491898278	23.45805131924851	25.402371157401866
5	33.92782076612206	29.66271666376194	15.656409654613453	20.753052915502547
6	17.455124952658345	41.15944211240195	17.2379290352278	24.147503899711907
7	20.253816712648018	28.90300681235505	20.066158080389172	30.777018394607765
8	15.475881733923764	33.112115250485715	23.113794355216985	28.298208660373536
9	19.53963808834946	36.796084626878454	27.46085714557634	16.203420139195742
10	27.746956899101704	27.968121145696568	17.344869982835913	26.940051972365815
11	31.612961940745958	22.435269492910372	7.420759172874369	38.531009393469304
12	22.497418249232656	26.436439560689816	23.503478916063212	27.56266327401432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	807.0
1	807.0
2	807.0
3	807.0
4	2145.0
5	3483.0
6	3483.0
7	3483.0
8	3483.0
9	3483.0
10	3483.0
11	3483.0
12	3483.0
13	8763.5
14	14044.0
15	14044.0
16	14044.0
17	14044.0
18	14044.0
19	14044.0
20	14044.0
21	29786.5
22	45529.0
23	45529.0
24	45529.0
25	45529.0
26	45529.0
27	45529.0
28	45529.0
29	104278.0
30	163027.0
31	163027.0
32	163027.0
33	163027.0
34	163027.0
35	163027.0
36	163027.0
37	163027.0
38	1792983.0
39	3422939.0
40	3422939.0
41	3422939.0
42	3422939.0
43	3422939.0
44	3422939.0
45	3422939.0
46	3615588.0
47	3808237.0
48	3808237.0
49	3808237.0
50	3808237.0
51	3808237.0
52	3808237.0
53	3808237.0
54	3947758.0
55	4087279.0
56	4087279.0
57	4087279.0
58	4087279.0
59	4087279.0
60	4087279.0
61	4087279.0
62	4087279.0
63	2129793.0
64	172307.0
65	172307.0
66	172307.0
67	172307.0
68	172307.0
69	172307.0
70	172307.0
71	106142.5
72	39978.0
73	39978.0
74	39978.0
75	39978.0
76	39978.0
77	39978.0
78	39978.0
79	24015.5
80	8053.0
81	8053.0
82	8053.0
83	8053.0
84	8053.0
85	8053.0
86	8053.0
87	8053.0
88	4783.5
89	1514.0
90	1514.0
91	1514.0
92	1514.0
93	1514.0
94	1514.0
95	1514.0
96	1247.0
97	980.0
98	980.0
99	980.0
100	980.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	8.497492857219942E-6
9	8.497492857219942E-6
10	0.0
11	8.497492857219942E-6
12	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.1768177E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.9967467953038542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.13275441547793	3.042833422277168
2	10.633240894162398	0.849967429348749
3	3.747487537845775	0.44933276421978696
4	2.0821228150736566	0.33286870754298703
5	1.3158882761358999	0.26296361253120354
6	0.8708535504339506	0.2088348682125525
7	0.717585246564977	0.20076045741961288
8	0.5197631290130809	0.16618892961601078
9	0.41792105803759044	0.1503292184461562
>10	2.9713112974174094	2.3425857410213435
>50	0.2925124854836694	0.8043854937862388
>100	0.2190402298856765	1.9013586314605284
>500	0.050209892125978435	1.413994250349337
>1k	0.02302980056785401	1.412320789194611
>5k	0.0014651867473340578	0.44702044949615
>10k+	0.004814185026954762	86.01425523507754
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGCGCAGATAC	684195	5.813942125445598	No Hit
ATTTCGACATGC	659572	5.604708358822271	No Hit
TAATGACCACGC	630918	5.361221198491491	No Hit
GCGGATGTGACT	619544	5.2645707147334715	No Hit
AATCAGTCTCGT	608635	5.171871565154059	No Hit
TGAGAACCTCCA	594573	5.052379820595832	No Hit
AGCAGTCGCGAT	567540	4.822667096186606	No Hit
CTTATCAAGGCG	565043	4.801448856522128	No Hit
ACCGTGCATAGG	561195	4.768750504007545	No Hit
TGCCCATTTAAG	546936	4.647584753356446	No Hit
GGAGACAAGGGA	537103	4.564028906091402	No Hit
AGTTCAGACGCT	532312	4.5233174178124615	No Hit
GAGTGAGTACAA	512162	4.35209293673948	No Hit
CAGGGTGATACC	459493	3.904538485442562	No Hit
AACGGATAAACG	446327	3.7926604944844047	No Hit
TGCCTACAAGCA	390180	3.3155517630300766	No Hit
ATCGAATGCGGC	362074	3.076721228785053	No Hit
GCACACACGTTA	351075	2.983257304848491	No Hit
TTCAGACTACCA	321965	2.7358952877748184	No Hit
CACGCCATAATG	299093	2.541540631144484	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAGT	130	3.6452548E-9	6.0000005	1
AACGTAT	65	0.0012618836	6.0000005	1
GTACAGA	95	3.4293553E-6	6.0000005	2
GTCGAAT	65	0.0012618836	6.0000005	1
GTTTAGG	65	0.0012618836	6.0000005	6
CGTGAGT	130	3.6452548E-9	6.0000005	2
ACCGAAT	95	3.4293553E-6	6.0000005	1
GCGCCGA	95	3.4293553E-6	6.0000005	3
AGGGTAA	65	0.0012618836	6.0000005	2
TACGGAC	65	0.0012618836	6.0000005	1
TTATCAG	95	3.4293553E-6	6.0000005	2
TGGCCAC	65	0.0012618836	6.0000005	4
TCGGACT	65	0.0012618836	6.0000005	2
TGACCAG	130	3.6452548E-9	6.0000005	4
GTTCGAC	95	3.4293553E-6	6.0000005	2
AATGAAC	65	0.0012618836	6.0000005	2
GTAGACA	65	0.0012618836	6.0000005	1
CAAGGGT	95	3.4293553E-6	6.0000005	6
CAAGGGC	205	0.0	6.0000005	6
GCTTAGG	205	0.0	6.0000005	6
>>END_MODULE