FastQCFastQC Report
Fri 25 Sep 2015
000000000-AJJF6 l01n02.332000000349b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJJF6 l01n02.332000000349b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11768177
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGCAGATAC6841955.813942125445598No Hit
ATTTCGACATGC6595725.604708358822271No Hit
TAATGACCACGC6309185.361221198491491No Hit
GCGGATGTGACT6195445.2645707147334715No Hit
AATCAGTCTCGT6086355.171871565154059No Hit
TGAGAACCTCCA5945735.052379820595832No Hit
AGCAGTCGCGAT5675404.822667096186606No Hit
CTTATCAAGGCG5650434.801448856522128No Hit
ACCGTGCATAGG5611954.768750504007545No Hit
TGCCCATTTAAG5469364.647584753356446No Hit
GGAGACAAGGGA5371034.564028906091402No Hit
AGTTCAGACGCT5323124.5233174178124615No Hit
GAGTGAGTACAA5121624.35209293673948No Hit
CAGGGTGATACC4594933.904538485442562No Hit
AACGGATAAACG4463273.7926604944844047No Hit
TGCCTACAAGCA3901803.3155517630300766No Hit
ATCGAATGCGGC3620743.076721228785053No Hit
GCACACACGTTA3510752.983257304848491No Hit
TTCAGACTACCA3219652.7358952877748184No Hit
CACGCCATAATG2990932.541540631144484No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGT1303.6452548E-96.00000051
AACGTAT650.00126188366.00000051
GTACAGA953.4293553E-66.00000052
GTCGAAT650.00126188366.00000051
GTTTAGG650.00126188366.00000056
CGTGAGT1303.6452548E-96.00000052
ACCGAAT953.4293553E-66.00000051
GCGCCGA953.4293553E-66.00000053
AGGGTAA650.00126188366.00000052
TACGGAC650.00126188366.00000051
TTATCAG953.4293553E-66.00000052
TGGCCAC650.00126188366.00000054
TCGGACT650.00126188366.00000052
TGACCAG1303.6452548E-96.00000054
GTTCGAC953.4293553E-66.00000052
AATGAAC650.00126188366.00000052
GTAGACA650.00126188366.00000051
CAAGGGT953.4293553E-66.00000056
CAAGGGC2050.06.00000056
GCTTAGG2050.06.00000056
Produced by FastQC (version 0.11.2)